A double staining method for the detection of a macrophage surface marker and nonspecific esterase activity

1988 ◽  
Vol 111 (1) ◽  
pp. 147-151 ◽  
Author(s):  
Francisco Leyva-Cobián ◽  
Reyes Flores
Keyword(s):  
Esterase Activity ◽  
Staining Method ◽  
Surface Marker ◽  
Nonspecific Esterase ◽  
Double Staining ◽  
Double Staining Method ◽  
Nonspecific Esterase Activity

Enzyme histochemical application and backscattered electron imaging to visualize the distribution of lymphatic capillaries

1990 ◽  
Vol 48 (3) ◽  
pp. 880-881
Author(s):  
Seiji Kato
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Reaction Products ◽  
Blood Capillaries ◽  
Backscattered Electron Imaging ◽  
Histochemical Observation ◽  
Backscattered Electron ◽  
Double Staining Method ◽  
Electron Imaging ◽  
Cryostat Sections

Previously, the author repeatedly confirmed the higher 5’-nucleotidase (5’-Nase) and lower alkaline phoaphatase (ALPase) activities in the wall of lymphatic capillaries reacted with the lead-based method relative to those of blood capillaries. The ALPase, on the other hand, is markedly higher in blood capillaries than in lymphatics. On the basis of these enzyme characteristics, the author has developed a 5’-Nase— ALPase double staining method to differentiate small lymphatics from blood capillaries at the level of the light microcsopy. Furthermore, we applied it to histochemical observation of the lead-containing reaction products of 5’-Nase in lymphatics on the same or adjacent cryostat sections using backscattered electron imaging (BEI) in scanning electron microscope (SEM). This paper presents a new applicability of 5’-Nase histochemistry by BEI-SEM to demonstrate the distribution of lymphatic capillaries in tissue blocks.

scholarly journals Immunohistochemical/histochemical double staining method in the study of the columnar metaplasia of the oesophagus

2014 ◽  
Vol 58 (1) ◽  
Author(s):  
D. Cabibi ◽  
A.G. Giannone ◽  
C. Mascarella ◽  
C. Guarnotta ◽  
M. Castiglia ◽  
...  
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Columnar Metaplasia ◽  
Double Staining Method

scholarly journals Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls

2008 ◽  
Vol 52 (No. 5) ◽  
pp. 186-192 ◽  
Author(s):  
I. Valocky ◽  
J. Legath ◽  
L. Lenhardt ◽  
G. Lazar ◽  
F. Novotny
Keyword(s):  
Alkaline Phosphatase ◽  
Epithelial Cells ◽  
Polychlorinated Biphenyls ◽  
Esterase Activity ◽  
Inhibitory Effect ◽  
Control Group ◽  
Nonspecific Esterase ◽  
Enzymatic System ◽  
Nonspecific Esterase Activity ◽  
Observation Period

The objective of this study was to examine the alkaline, acidic phosphatase and nonspecific esterase activity in the epithelial cells of oviducts after exposure to polychlorinated biphenyls (PCBs) at the time of puerperium. PCBs were administered in the last days of pregnancy and during early puerperium. Animals in the experimental group were exposed to Delor 105 at a dose of 100 &mu;g/kg/day and were euthanised on Day 17 postpartum (<i>n</i> = 4), i.e. 5 days after the termination of 30-day PCB administration; on Day 25 postpartum (<i>n</i> = 5), i.e. 17 days from the last PCB administration and on Day 34 postpartum (<i>n</i> = 5), which corresponded to Day 28 from the completion of PCB administration. Ewes in the control group were euthanised on Day 17 (<i>n</i> = 3), Day 25 (<i>n</i> = 4) and Day 34 (<i>n</i> = 4) postpartum. The authors demonstrated the inhibitory effect of PCB on the enzymatic system of the oviduct during the puerperal period. The alkaline phosphatase, acidic phosphatase and nonspecific esterase activity in the oviductal epithelial cells during a 34-day observation period exhibited a rising trend (<i>P</i> < 0.001 vs. <i>P</i> < 0.001 vs. <i>P</i> < 0.01) in the control group of animals. Experimental animals exposed to the 30-day PCB administration (Delor 105) showed a stagnant tendency (<i>P</i> > 0.05) in alkaline phosphatase while acidic phosphatase and nonspecific esterase activity (<i>P</i> > 0.05) dropped even below the level of their activity values in the control group. It is essential to continue to monitor the effect of pollutants in exposed industrial areas on reparative and regenerative processes in puerperium and their possible impact on reproductive performance.

Identification of anti-golgi antibody by double-staining method with antibody to β-cop

1993 ◽  
Vol 6 (1) ◽  
pp. 77
Author(s):  
Hong-Shang Hong ◽  
Yu-Shung Lee ◽  
Heng-Leong Chan ◽  
Tseng-tong Kuo
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Double Staining Method

scholarly journals Cloned mouse cells with natural killer function and cloned suppressor T cells express ultrastructural and biochemical features not shared by cloned inducer T cells.

1983 ◽  
Vol 157 (3) ◽  
pp. 843-861 ◽  
Author(s):  
A M Dvorak ◽  
S J Galli ◽  
J A Marcum ◽  
G Nabel ◽  
H der Simonian ◽  
...  
Keyword(s):  
T Cells ◽  
Natural Killer ◽  
Esterase Activity ◽  
Membrane Receptors ◽  
Surface Glycoprotein ◽  
Nonspecific Esterase ◽  
Suppressor T Cells ◽  
Cytoplasmic Granules ◽  
Plasma Membrane Receptors ◽  
Nonspecific Esterase Activity

We have examined the morphology, cytochemistry, and biochemistry of mouse leukocyte subsets by analyzing cloned leukocyte populations specialized to perform different immunologic functions. Cloned cells expressing high-affinity plasma membrane receptors for IgE and mediating natural killer (NK) lysis and cloned antigen-specific suppressor T cells contained prominent osmiophilic cytoplasmic granules similar by ultrastructure to those of mouse basophils. Both clones also incorporated 35SO4 into granule-associated sulfated glycosaminoglycans, expressed a characteristic ultrastructural pattern of nonspecific esterase activity, incorporated exogenous [3H]5-hydroxytryptamine, and contained cytoplasmic deposits of particulate glycogen. By contrast, cloned inducer T cells lacked cytoplasmic granules and glycogen, incorporated neither 35SO4 nor [3H]5-hydroxytryptamine, and differed from the other clones in pattern of nonspecific esterase activity. These findings establish that certain cloned cells with NK activity and cloned suppressor T cells express morphologic and biochemical characteristics heretofore associated with basophilic granulocytes. However, these clones differ in surface glycoprotein expression and immunologic function, and the full extent of the similarities and differences among these populations and basophils remains to be determined.

scholarly journals Immunocytochemical localization of the posterior lobe hormones and of their carrier proteins.

1980 ◽  
Vol 28 (5) ◽  
pp. 469-471 ◽  
Author(s):  
F Vandesande ◽  
K Dierickx ◽  
N Goossens
Keyword(s):  
Staining Method ◽  
Posterior Lobe ◽  
Serial Sections ◽  
Double Staining ◽  
Carrier Proteins ◽  
Tissue Sections ◽  
Tissue Antigens ◽  
Staining Methods ◽  
Double Staining Method ◽  
The One

Serial sections of vertebrate hypothalami were stained with the immunocytochemical peroxidase-antiperoxidase method. In addition to the single staining method, our double staining method was used, which enabled us to visualize two tissue antigens in single tissue sections. In both staining methods, differentially adsorbed antineurohypophysial hormone sera, anti-somatostatin serum and anti-bovine neurophysin sera were used. The results confirm the one hormone, one neuron hypothesis.

Results of a double staining method for DNA and RNA

1968 ◽  
Vol 55 (10) ◽  
pp. 496-497 ◽  
Author(s):  
Sibdas Ghosh ◽  
Renate Lettr�
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Dna And Rna ◽  
Double Staining Method

scholarly journals Combined beta-galactosidase and immunogold/silver staining for immunohistochemistry and DNA in situ hybridization.

1990 ◽  
Vol 38 (3) ◽  
pp. 325-329 ◽  
Author(s):  
W van den Brink ◽  
C van der Loos ◽  
H Volkers ◽  
R Lauwen ◽  
F van den Berg ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Silver Staining ◽  
Staining Method ◽  
Double Staining ◽  
Reaction Products ◽  
Silver Enhancement ◽  
Silver Staining Method ◽  
Double Staining Method ◽  
Beta Galactosidase

A combination of beta-galactosidase enzyme and the immunogold/silver staining method was studied for evaluation of double-staining experiments. Applications are shown for immunohistochemical double staining using two monoclonal antibodies and for combined immunohistochemistry and DNA in situ hybridization in one tissue section. The following advantages for the present double-staining method were evaluated: superior sensitivity of the immunogold/silver staining method for at least one epitope, which also allows detection of biotinylated DNA probes. The structure of the indolyl precipitate after revelation of beta-galactosidase activity did not show a concealing effect during a sequential double-staining method, as compared with the visualization of peroxidase with diaminobenzidine. These factors, and the sharply contrasting colored reaction products of beta-galactosidase (blue-green) and the immunogold/silver staining method including silver enhancement (brown-black), allow clear distinction of mixed-stained cell constituents.

Sign in / Sign up

Export Citation Format

Share Document