Modified Fujiwara reaction for the determination of trichloroacetic acid

1991 ◽  
Vol 43 (2) ◽  
pp. 109-111 ◽  
Author(s):  
Madhusri Bhattacharjee ◽  
Lata Cherian ◽  
V.K. Gupta
Keyword(s):  
Trichloroacetic Acid ◽  
Fujiwara Reaction

An Improved Enzymatic-Ultraviolet Method for Determination of Uric Acid in Flours

1966 ◽  
Vol 49 (5) ◽  
pp. 899-902
Author(s):  
N P Sen ◽  
D Morison Smith
Keyword(s):  
Uric Acid ◽  
Trichloroacetic Acid ◽  
Cereal Products

Abstract Main improvements in the enzymaticultraviolet method for uric acid in flours include elimination of the uricase precipitation by trichloroacetic acid, which was not reproducible, and destruction of uricaselike material in flour before extraction. The method is capable of measuring as low as 3.2 mg uric acid per 100 g flour and is applicable to a variety of cereal products such as rice, rye, corn and soya flours, as well as barley, oats, etc.

Determination of Residual Cephalexin in Chick Tissues by Bacillus stearothermophilus Paper Disc Assay

1988 ◽  
Vol 71 (2) ◽  
pp. 337-340
Author(s):  
Junya Okada ◽  
Ikuji Higuchi ◽  
Sadao Kondo ◽  
Bun-Ichi Saito
Keyword(s):  
Detection Limit ◽  
Trichloroacetic Acid ◽  
Bacillus Stearothermophilus ◽  
Test Organism ◽  
Acid Extract ◽  
Average Recovery ◽  
Ad Libitum ◽  
Paper Disc ◽  
Disc Assay

Abstract A paper disc method is described for determination of residual cephalexin (CEX) in chick tissues. A trichloroacetic acid extract of plasma and tissues is chromatographed on a macroreticular resin (Diaion HP-20) column to remove endogenous antibacterial substances interfering with the assay. The eluate is evaporated to dryness and the residue, dissolved in methanol-water (1 + 2), is subjected to a paper disc assay using Bacillus stearothermophilus var. calidolactis C953 NIZO as a test organism. The detection limit was 0.0375 ppm in tissue; the average recovery of CEX ranged from 72.4% in skin to 90.4% in plasma. Water containing 200 or 500 mg/L of CEX was given ad libitum to 2-week-old chicks for 10 days; the highest levels of CEX were found in the kidney, and the lowest were found in muscle at 0 h of withdrawal. CEX disappeared from most tissues at 24 h after withdrawal except from skin of chicks given 500 mg/ L. However, the drug was not detected in the skin at 48 h after withdrawal.

Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: A comparison with fluorescence detection

2011 ◽  
Vol 76 (4) ◽  
pp. 277-294 ◽  
Author(s):  
Roman Kanďár ◽  
Pavla Žáková ◽  
Miroslava Marková ◽  
Halka Lotková ◽  
Otto Kučera ◽  
...  
Keyword(s):  
Detection Limit ◽  
Whole Blood ◽  
Trichloroacetic Acid ◽  
Signal To Noise Ratio ◽  
Glutathione Disulfide ◽  
Simple Method ◽  
Human Whole Blood ◽  
Coulometric Detection ◽  
Coefficients Of Variation

We describe a relatively simple method for the determination of glutathione (GSH) and glutathione disulfide (GSSG) in human whole blood. We have used an HPLC with coulometric electrochemical detection for the simultaneous measurement of GSH and GSSG. Diluted and filtered trichloroacetic acid extracts were injected directly into the HPLC system and were eluted isocratically on a Polaris 5u C18-A, 250 × 4.6 mm analytical column. Glutathione in samples extracted with trichloroacetic acid and diluted with 1.0 mMhydrochloric acid was stable at 4 °C for at least 8 h. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked whole blood samples were at intervals 91.6–97.6% for GSH and 85.0–104.4% for GSSG. The linear range is 5.0–2000.0 μmol/l, with a detection limit of 2.1 μmol/l (signal-to-noise ratio = 3) for GSH and 2.0–250.0 μmol/l, with a detection limit of 0.9 μmol/l for GSSG.

THE DETERMINATION OF TERMINAL PROTEIN-BOUND FUCOSE

1965 ◽  
Vol 43 (11) ◽  
pp. 1807-1811 ◽  
Author(s):  
G. Gyorky ◽  
J. C. Houck
Keyword(s):  
Protein Degradation ◽  
Spectrophotometric Determination ◽  
Trichloroacetic Acid ◽  
Degradation Products ◽  
Dilute Acid ◽  
Terminal Protein ◽  
Residual Protein ◽  
Color Yield ◽  
Fucose Content

The spectrophotometric determination of protein-bound fucose is badly compromised by spurious chromagens developed from protein degradation products. To minimize the contribution of these spurious products to the color yield of fucose, the glycoprotein was partially hydrolyzed in dilute acid, thus releasing the terminal carbohydrate from the protein moieties, and the residual protein was removed with trichloroacetic acid. That the fucose content of this supernatant was real was confirmed by paper chromatography and spectral studies.The spurious chromagens were shown to result from the interaction of protein degradation products and galactose.

Electrophoretic Method for Qualitative and Quantitative Analysis of Gelling and Thickening Agents

1982 ◽  
Vol 65 (3) ◽  
pp. 745-752
Author(s):  
U D O Pechanek ◽  
Gernot Blaicher ◽  
Werner Pfannhauser ◽  
Herbert Woidich
Keyword(s):  
Trichloroacetic Acid ◽  
Starch Degradation ◽  
Migration Behavior ◽  
Qualitative And Quantitative Analysis ◽  
Electrophoretic Method ◽  
Gelling Agents ◽  
Qualitative And Quantitative ◽  
Removal Of Fat ◽  
Thickening Agents

Abstract An electrophoretic method has been developed for qualitative and quantitative determination of all common thickening and gelling agents. Thickeners were identified by their migration behavior, their staining ability, and the characteristic shapes of their electrophoretic zones, and then quantitated in a scanner. A method is also reported for isolating thickeners from food, including removal of fat and dyes by dioxane, enzymatic starch degradation, removal of protein by trichloroacetic acid, and precipitation of the polysaccharides by absolute ethanol. Isolation of gelatin is also described.

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