In vitro uptake of organic ions by renal cortical tissue of rats treated acutely with 2,4,5-trichlorophenoxyacetic acid

An attempt has been made to study the action of aldosterone on tubular reabsorption of sodium using in vitro incubation of rat renal cortex slices. In normally hydrated animals neither adrenalectomy nor aldosterone, whether injected into the animal or added to the incubation medium, influenced significantly the rate and extent of slice sodium and water accumulation and potassium depletion. However, effects on other variables were found. After adrenalectomy, the oxygen consumption of the renal cortical tissue was decreased 10–13.5% and the ammonia production 27–33%. Unlike oxygen consumption, ammonia production was influenced by aldosterone; when the hormone was injected into rats the renal cortical ammonia production increased 27–65%. Ammonia concentrations in the incubation media could account for the progressive alkalinization observed with increasing time of incubation. Hydropenia resulted in an increased sodium accumulation and potassium depletion, and a decreased oxygen consumption and ammonia production by incubated slices from intact as well as adrenalectomized rats.

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IN VITRO UPTAKE AND BREAKDOWN OF TRITIATED LYSINE-VASOPRESSIN BY BOVINE NEUROHYPOPHYSEAL AND CORTICAL TISSUE

Acta Endocrinologica ◽

10.1530/acta.0.0670012 ◽

1971 ◽

Vol 67 (1) ◽

pp. 12-22 ◽

Cited By ~ 16

Author(s):

V. Pliška ◽

N. A. Thorn ◽

H. Vilhardt

Keyword(s):

Thin Layer ◽

Parietal Cortex ◽

Total Radioactivity ◽

Cortical Tissue ◽

Simple Manner ◽

Lysine Vasopressin ◽

Locke Solution ◽

Layer Chromatography ◽

In Vitro Uptake

ABSTRACT Pieces of tissue from the neurohypophysis and parietal cortex of cows were incubated in a modified Locke solution containing specifically tritiated lysine-vasopressin (LVP). After incubation, the uptake of radioactivity associated with LVP, 2 peptide split products and tyrosine as well as radioactivity in TCA insoluble proteins was determined. Thin-layer chromatography was used to identify the intact hormone and its split products. The total radioactivity as well as the radioactivity associated with LVP and the aminopeptidase-split products increased much more rapidly in the neurohypophysis than in the cortex. The tissue to medium ratio of radioactivity for LVP and the aminopeptidase-split products quickly became greater than one in the neurohypophysis, whereas it remained below one in the cortex. Radioactivity associated with TCA insoluble proteins quickly appeared. The temperature dependence of the appearance of radioactivity in LVP and the aminopeptidase-split products in both the neurohypophysis and the cortex was complicated, whereas the appearance of radioactivity in tyrosine increased with temperature in a simple manner.

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Slowly dialyzable stimulators of renal protein synthesis in urine

AJP Renal Physiology ◽

10.1152/ajprenal.1979.237.4.f299 ◽

1979 ◽

Vol 237 (4) ◽

pp. F299-F306

Author(s):

R. H. Harris ◽

C. F. Best

Keyword(s):

Protein Synthesis ◽

Cellular Uptake ◽

Renal Mass ◽

Total Protein Content ◽

Cortical Tissue ◽

Renal Growth ◽

Excretory Function ◽

Renal Cortical Tissue ◽

Renal Excretory Function

Previously, we demonstrated that continuous intravenous reinfusion of half the urine output (1/2UR) in rats for 1 wk led to increased renal mass. This suggested that reduced renal excretory function, or the retention of urinary factors, was capable of stimulating renal growth. The present study was designed to examine renal protein synthesis during the early phase of this growth and to better define the nature of the stimuli. Compared with matched sham-manipulated control rats, rats subjected to 24 h of 1/2UR displayed significant increases in both the incorporation of tritiated leucine into protein and in the cellular uptake of leucine by renal cortical tissue in vitro. In addition, total protein content of the kidneys, but not of the liver, was significantly increased after 24 h of 1/2UR. Dialysis of urine prior to its reinfusion did not diminish, but rather augmented, the incorporation of leucine into renal protein. These results suggest that renal protein synthesis can be stimulated by the retention of factors in the urine that are poorly dialyzable.

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Functional maturation of renal PAH transport in the dog

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y70-029 ◽

1970 ◽

Vol 48 (3) ◽

pp. 169-175 ◽

Cited By ~ 26

Author(s):

J. B. Hook ◽

H. E. Williamson ◽

G. H. Hirsch

Keyword(s):

Transport Mechanisms ◽

Cortical Tissue ◽

Functional Maturation ◽

In Vitro Systems ◽

Anesthetized Dogs ◽

Renal Cortical Tissue ◽

Slice Method ◽

Do So

Most investigations dealing with the development of renal transport mechanisms have utilized in vitro systems for measuring transport. The purpose of this investigation was to observe the pattern of development of renal PAH transport in vivo by measuring TmPAH in anesthetized dogs ranging in age from 1 week to adult. Puppies from each litter were studied at 1, 2, 4, 6, and 8 weeks of age in acute experiments. The maximal ability to transport PAH was determined by increasing the plasma concentration in a stepwise manner until Tm was exceeded. The Tm data obtained were compared with transport data obtained from other animals at similar ages using the in vitro slice method of Cross and Taggart. TmPAH was found to increase from 1 week to 8 weeks of age. At 8 weeks TmPAH was still less than in the adult whether based on kidney weight or body weight. In contrast, when measured in vitro, PAH transport (S/M) was found to increase to a maximum at 4 weeks and decline to adult levels. These data demonstrate that the cellular function (S/M) and apparent functional mass (Tm) of renal cortical tissue are low at birth and increase with age, but do so at different rates.

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On the Role of Transferrin in 67Ga Uptake by Tumor Cells

Nuklearmedizin ◽

10.1055/s-0038-1629447 ◽

1988 ◽

Vol 27 (04) ◽

pp. 151-153

Author(s):

P. Thouvenot ◽

F. Brunotte ◽

J. Robert ◽

L. J. Anghileri

Keyword(s):

Specific Binding ◽

Subcellular Fractionation ◽

Animal Blood ◽

Tumor Bearing ◽

Cell Structures ◽

The Difference ◽

Sarcoma Cells ◽

In Vitro Uptake

In vitro uptake of 67Ga-citrate and 59Fe-citrate by DS sarcoma cells in the presence of tumor-bearing animal blood plasma showed a dramatic inhibition of both 67Ga and 59Fe uptakes: about ii/io of 67Ga and 1/5o of the 59Fe are taken up by the cells. Subcellular fractionation appears to indicate no specific binding to cell structures, and the difference of binding seems to be related to the transferrin chelation and transmembrane transport differences

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IN VITRO UPTAKE OF TRITIATED OESTRADIOL BY THE ANTERIOR HYPOTHALAMUS AND HYPOPHYSIS OF THE RAT

Acta Endocrinologica ◽

10.1530/acta.0.0640687 ◽

1970 ◽

Vol 64 (4) ◽

pp. 687-695 ◽

Cited By ~ 10

Author(s):

Junzo Kato

Keyword(s):

Incubation Medium ◽

Posterior Hypothalamus ◽

Anterior Hypothalamus ◽

Ovariectomized Rats ◽

Free Medium ◽

Cortex Cerebri ◽

Anterior Hypophysis ◽

In Vitro Uptake

ABSTRACT The anterior, middle, and posterior hypothalamus, the cortex cerebri, the anterior hypophysis as well as the diaphragm of adult ovariectomized rats were incubated in vitro with tritiated 17β-oestradiol. The uptake of tritiated oestradiol was differentially distributed intracerebrally with higher accumulation in the anterior hypothalamus and the hypophysis. Lowering the temperature of the incubation medium caused a reduction in the uptake of radioactivity by the anterior hypothalamus as compared to that found in other brain tissues. Tritiated oestradiol taken up in vitro by the anterior hypothalamus and the hypophysis tended to be retained after further incubation in a steroid-free medium. The addition of non-radioactive 17β-oestradiol to the medium inhibited the uptake of tritiated oestradiol by these tissues. Moreover, pretreatment with non-radioactive 17β-oestradiol in vivo prevented the preferential accumulation of tritiated oestradiol in vitro in the anterior hypothalamus and the hypophysis. These results indicate that oestradiol is preferentially taken up in vitro by the anterior hypothalamus and the hypophysis of the rat.

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Interaction between parathyroid hormone and prostaglandin E1 on cyclic AMP metabolism in rat kidney

Acta Endocrinologica ◽

10.1530/acta.0.0930339 ◽

1980 ◽

Vol 93 (3) ◽

pp. 339-345 ◽

Cited By ~ 2

Author(s):

Naokazu Nagata ◽

Yuriko Ono ◽

Narimichi Kimura

Keyword(s):

Parathyroid Hormone ◽

Cyclic Amp ◽

Adenylate Cyclase ◽

Rat Kidney ◽

Prostaglandin E ◽

Cortical Tissue ◽

Newborn Rat ◽

Simultaneous Addition ◽

Renal Cortical Tissue ◽

Subsequent Addition

Abstract. The interaction between parathyroid hormone (PTH) and prostaglandin E1 (PGE1) in influencing cyclic AMP metabolism in rat renal cortical tissue was examined. PTH and PGE1 stimulated additively the adenylate cyclase activity in the homogenate of the tissue. Both PTH and PGE1 enhanced the level of cyclic AMP in the incubated renal cortical tissue, but the effect of their simultaneous addition did not exceed the effect induced by PTH alone. Cyclic AMP accumulated in the incubation medium by stimulation by PTH was decreased by the simultaneous addition of PGE1. When the tissue was pre-incubated for 30 min with 2 to 10 μg/ml of PGE1, the magnitude of the increase of cyclic AMP caused by PTH subsequently added was lessened. However, the response to PTH of adenylate cyclase preparation obtained from the homogenate of PGE1-pre-treated tissue was not decreased. When first PTH was added to the incubating renal cortical tissue, the subsequent addition of PGE1 accelerated the decrease of cyclic AMP content in the tissue and decreased the amount of cyclic AMP released from the tissue. The interaction of PTH and PGE1 on cyclic AMP metabolism in the renal cortical tissue was in contrast to that seen in newborn rat calvaria where PGE1 and PTH acted additively in enhancing the level of cyclic AMP.

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