The waste-product theory of aging: Cell division rate as a function of waste volume

The goal of the present study was to evaluate the cytotoxicity and genotoxicity of artificial synthetic flavoring agents cookie and tutti-frutti. To this end, root meristem cells of Allium cepa L. were exposed to these substances in exposure times of 24 and 48 hour using individual doses of 0.3; 0.6 and 0.9 mL and doses combined as follows: 0.3 mL + 0.3 mL; 0.6 mL and 0.9 mL + 0.6 mL + 0.9 mL. After applying the treatments, root meristems were fixed, hydrolyzed, stained and analyzed a total of 5,000 cells using an optical microscope to evaluate each dose and combined treatment. All three doses of cookie flavoring and combined treatments significantly inhibited cell division of the tissue studied. Doses of tutti-frutti caused no change in cell division rate. In addition, doses of both flavorings and treatments combining these solutions induced cell aberrations in a significant number of cells to the A. cepa system. Therefore, under these analytical conditions, cookie flavoring and combined doses were cytotoxic and genotoxic, and tutti-frutti flavoring, although non-cytotoxic, demonstrated genotoxic action.

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Effect of Aluminium on Root Growth, Cell-division Rate and Mineral Element Contents in Roots of Vigna unguiculata Genotypes

Zeitschrift für Pflanzenphysiologie ◽

10.1016/s0044-328x(83)80199-8 ◽

1983 ◽

Vol 109 (2) ◽

pp. 95-103 ◽

Cited By ~ 99

Author(s):

W.J. Horst ◽

A. Wagner ◽

H. Marschner

Keyword(s):

Cell Division ◽

Root Growth ◽

Vigna Unguiculata ◽

Mineral Element ◽

Division Rate ◽

Cell Division Rate ◽

Element Contents

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Effect of Nitrogen and Periphyton Extract on the Growth of Nostoc sphaericum in Cultures

ISRN Ecology ◽

10.5402/2012/935476 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8

Author(s):

Itzel Becerra-Absalón ◽

Thomas Buhse ◽

Carlos Polanco ◽

Rosaluz Tavera

Keyword(s):

Cell Division ◽

Deciduous Forest ◽

Tropical Deciduous Forest ◽

Division Rate ◽

Wetland Ecosystems ◽

Statistical Verification ◽

Solid Media ◽

Cell Division Rate ◽

Growth Differences ◽

The Impact

Nostoc sphaericum shows marked growth differences in two Mexican wetland ecosystems consisting of rain forest and tropical deciduous forest, respectively. The amount of nitrogen and periphyton extract dominated by other Cyanoprokaryota had been identified as the most obvious differences between these two ecosystems. We studied the impact of these variables on the physiology and morphology of N. sphaericum. that is, the chlorophyll-a content of the thalli and the changes in the size of the trichomes as well as the cell division rate. Our results combined with a statistical verification indicate that the cell division rate of N. sphaericum with solid media is neither stimulated by nitrogen nor by accompanying cyanoprokaryotes and therefore is assumed to have no impact on the thalli observed in situ. However, these two variables are affecting the size of both the trichomes and the thalli, thus suggested to cause the observed growth differences between the two wetlands.

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