Partial characterization of different cell types found in the Xenopuslaevis lymphoreticular tumor based on the presence or absence of surface immunoglobulins and Fc receptor molecules

Of all the significant researches that have taken place in isolation, culture and characterization of mesenchymal stem cells (MSCs), the field of kidney-derived mesenchymal stem cells (KMSCs) in Tibetan mastiff is still a blank. Therefore, the purpose of this study is to isolate, culture and characterize the Tibetan mastiff KMSCs. The KMSCs were successfully isolated from one-day year old Tibetan mastiff kidney, cultured for 16 passages and distinguished by two methods: immunofluorescence staining and RT-PCR. The Tibetan mastiff KMSCs expressed specific surface marker genes (VIM, CD44, FN1, CD90, CD109, CD73, FN1) and kidney marker gene PAX2. The proliferation ability of Tibetan mastiff KMSCs was measured through cell count and clonality. Furthermore, cells differentiated into different cell types (hepatocellular cells, osteogenic cells, adipogenic cells and chondrogenic cells) under special induced medium, and the marker genes of induced cells were identified with Immunofluorescence staining and RT-PCR. All of these results indicated that the Tibetan mastiff KMSCs were obtained successfully, which possessed certain characteristics of multipotent stem cells. Therefore, MSCs in Tibetan mastiff kidney hold potential for clinical applications for regenerative therapy and their further studies are waiting to be required to investigate their functions.

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Isolation and partial characterization of biologically active Fc receptor of chicken red cells

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/0304-4165(87)90046-8 ◽

1987 ◽

Vol 923 (3) ◽

pp. 381-388 ◽

Cited By ~ 3

Author(s):

Marcela A. Manghi ◽

Stella M. Venturiello ◽

M. Inés Gutiérrez ◽

Marina Etchevierrigaray ◽

Ricardo A. Margni

Keyword(s):

Fc Receptor ◽

Red Cells ◽

Biologically Active ◽

Partial Characterization

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Neonatal Fc receptor (FcRn) – not only transporter of maternal IgG

Postępy Higieny i Medycyny Doświadczalnej ◽

10.5604/01.3001.0012.2094 ◽

2018 ◽

Vol 72 ◽

pp. 701-727

Author(s):

Joanna E. Mikulska

Keyword(s):

Mhc Class I ◽

Current Knowledge ◽

Cell Types ◽

Fc Receptor ◽

Class I ◽

Neonatal Fc Receptor ◽

The Status ◽

And Function ◽

Different Cell Types ◽

Maternal Igg

The neonatal Fc receptor, (FcRn) is a transmembrane, heterodimeric glycoprotein with a structure similar to MHC class I molecules. In contrast to MHC class I antigens, FcRn does not bind to peptides (antigens) but interacts with the Fc fragment of IgG and albumin. The FcRn-IgG interaction as well as the FcRn-albumin interaction occur at acidic pH (optimally at pH 5.0-6.5) but not in physiological environment. These two ligands bind to distinct binding sites on the receptor molecule and by different mechanisms. Now, it is known that the expression of FcRn is not restricted to sites involved in the transport of maternal IgG, and this receptor is not responsible only for transfer the passive immunity from mother to the offspring. But FcRn has a much broader range of expression and function, throughout life and in many different cell types and tissues of humans and animals. This review summarizes the status of our knowledge on the expression, interaction with ligands and functions of the neonatal Fc receptor. This article shows also the possibilities of utilizing a current knowledge on FcRn for therapeutic purposes.

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αT-Catenin: a novel tissue-specific β-catenin-binding protein mediating strong cell-cell adhesion

Journal of Cell Science ◽

10.1242/jcs.114.17.3177 ◽

2001 ◽

Vol 114 (17) ◽

pp. 3177-3188 ◽

Cited By ~ 1

Author(s):

Barbara Janssens ◽

Steven Goossens ◽

Katrien Staes ◽

Barbara Gilbert ◽

Jolanda van Hengel ◽

...

Keyword(s):

Cell Adhesion ◽

Immunohistochemical Analysis ◽

Cell Types ◽

Resistant Cell ◽

Colon Carcinoma Cells ◽

Intercalated Discs ◽

Different Cell Types ◽

Cell Adhesion Proteins ◽

Cell Cell

Cadherins are major cell-cell adhesion proteins whose cytoplasmic domains bind to catenin proteins. Strong intercellular adhesion depends on linkage of the cadherin/catenin complex to the actin cytoskeleton via α-catenin. To date, it is not clear how different cell types achieve the variable strength of cell-cell adhesion clearly needed in a multicellular organism. Here, we report the cloning and molecular characterization of αT(testis)-catenin, a novel human cDNA encoding a protein with homology to both human αE(epithelial)-catenin and αN(neural)-catenin. Although originally discovered in testis, αT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human αT-catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. In cells transfected with αT-catenin cDNA, interaction with β-catenin was demonstrated by co-immunoprecipitation. Transfection of α-catenin-deficient colon carcinoma cells recruited E-cadherin and β-catenin to cell-cell contacts and functional cadherin-mediated cell-cell adhesion was restored in this way. Moreover, compaction of these cells was at least as prominent as in the case of cells expressing endogenous αE-catenin. We propose that αT-catenin is necessary for the formation of stretch-resistant cell-cell adhesion complexes, in particular, muscle cells.

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Origin and partial characterization of Fc receptor-bearing cells found within experimental carcinomas and sarcomas

International Journal of Cancer ◽

10.1002/ijc.2910150607 ◽

1975 ◽

Vol 15 (6) ◽

pp. 918-932 ◽

Cited By ~ 65

Author(s):

R. S. Kerbel ◽

H. F. Pross ◽

E. V. Elliott

Keyword(s):

Fc Receptor ◽

Partial Characterization

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Cell penetrating peptides can exert biological activity: a review

BioMolecular Concepts ◽

10.1515/bmc.2010.016 ◽

2010 ◽

Vol 1 (2) ◽

pp. 109-116 ◽

Cited By ~ 11

Author(s):

Jamie Brugnano ◽

Brian C. Ward ◽

Alyssa Panitch

Keyword(s):

Gene Expression ◽

Biological Activity ◽

Cell Membrane ◽

Recent Literature ◽

Cell Types ◽

Cell Penetrating Peptides ◽

Cell Penetrating ◽

Different Cell Types ◽

Traditional Understanding

AbstractCell penetrating peptides (CPPs) have been successful in delivering cargo into many different cell types and are an important alternative to other methods of permeation that might damage the integrity of the cell membrane. The traditional view of CPPs is that they are inert molecules that can be successfully used to deliver many cargos intracellularly. The goal of this review is to challenge this traditional understanding of CPPs. Recent literature has demonstrated that CPPs themselves can convey biological activity, including the alteration of gene expression and inhibition of protein kinases and proteolytic activity. Further characterization of CPPs is required to determine the extent of this activity. Research into the use of CPPs for intracellular delivery should continue with investigators being aware of these recent results.

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