In vitro colony formation of carp granulocytes using semi-solid agar culture system

1991 ◽  
Vol 15 ◽  
pp. S90
Keyword(s):  
Colony Formation ◽  
Culture System ◽  
Agar Culture ◽  
Solid Agar ◽  
Semi Solid

scholarly journals In Vitro Colony Formation of Carp Granulocytes in a Semi-Solid Agar Culture System.

1993 ◽  
Vol 28 (2) ◽  
pp. 59-63 ◽  
Author(s):  
Tadaaki Moritomo ◽  
Akihide Itou ◽  
Itaru Yamaguchi ◽  
Tasuku Watanabe
Keyword(s):  
Colony Formation ◽  
Culture System ◽  
Agar Culture ◽  
Solid Agar ◽  
Semi Solid

scholarly journals Enhancement of colony-stimulating activity production by lithium

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 263-267 ◽  
Author(s):  
WG Harker ◽  
G Rothstein ◽  
D Clarkson ◽  
JW Athens ◽  
JL Macfarlane
Keyword(s):  
Protein Synthesis ◽  
Lung Tissue ◽  
Colony Formation ◽  
Culture System ◽  
Agar Culture ◽  
Colony Stimulating Activity ◽  
New Protein ◽  
Stimulation Of

Since lithium causes granulocytosis in some patients, its effect upon granulocyte production was investigated using mouse marrow in the agar culture system. When lithium was added to semisolid cultures of mouse marrow, there was no stimulation of colony formation in the absence of colony-stimulating activity (CSA). In addition, lithium did not potentiate the action of already formed CSA. However, lithium did stimulate the production of CSA by lung tissue. Lithium enhancement of CSA production was blocked by puromycin, indicating that lithium action required active new protein synthesis. It was concluded that lithium promoted enhanced granulocyte production in vitro by stimulating the synthesis of CSA.

scholarly journals Enhancement of colony-stimulating activity production by lithium

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 263-267 ◽  
Author(s):  
WG Harker ◽  
G Rothstein ◽  
D Clarkson ◽  
JW Athens ◽  
JL Macfarlane
Keyword(s):  
Protein Synthesis ◽  
Lung Tissue ◽  
Colony Formation ◽  
Culture System ◽  
Agar Culture ◽  
Colony Stimulating Activity ◽  
New Protein ◽  
Stimulation Of

Abstract Since lithium causes granulocytosis in some patients, its effect upon granulocyte production was investigated using mouse marrow in the agar culture system. When lithium was added to semisolid cultures of mouse marrow, there was no stimulation of colony formation in the absence of colony-stimulating activity (CSA). In addition, lithium did not potentiate the action of already formed CSA. However, lithium did stimulate the production of CSA by lung tissue. Lithium enhancement of CSA production was blocked by puromycin, indicating that lithium action required active new protein synthesis. It was concluded that lithium promoted enhanced granulocyte production in vitro by stimulating the synthesis of CSA.

The Effects of Drugs that Cause Neutropenia upon Colony Formation by Bone Marrow Cells in Semi-Solid Agar

1974 ◽  
Vol 46 (5) ◽  
pp. 619-628
Author(s):  
A. Howell ◽  
Susan Chinn ◽  
T. M. Andrews ◽  
R. W. E. Watts
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Sodium Aurothiomalate ◽  
Response Curves ◽  
Agar Culture ◽  
Drug Concentrations ◽  
Solid Agar ◽  
Semi Solid

1. The effect of drugs that cause neutropenia has been tested upon normal bone marrow colony formation in semi-solid agar. 2. Thiouracil, methimazole and phenylbutazone do not cause inhibition whereas sodium aurothiomalate, 6-mercaptopurine and chloramphenicol cause dose-related inhibition of colony formation at or below drug concentrations found in serum during therapy. Chloramphenicol also inhibits mouse bone marrow, L cells and Hela cells in agar culture. 3. Dose—response curves have been analysed by the probit method and found to be reproducible.

scholarly journals Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

2018 ◽  
Vol 47 (5) ◽  
pp. 2109-2125 ◽  
Author(s):  
Zhaocong Yang ◽  
Yanfeng Zhang ◽  
Tingting Tang ◽  
Qinhua Zhu ◽  
Wanyue Shi ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Culture System ◽  
High Throughput Sequencing ◽  
Semi Solid ◽  
Pancreatic Cancer Stem Cells ◽  
Solid System

Background/Aims: Pancreatic cancer remains one of the deadliest human malignancies, the lethality of which may be attributed to the presence of pancreatic cancer stem cells (PCSCs), a small subpopulation of cells existing within pancreatic tumor with high carcinogenesis. Therefore, it is crucial to establish an efficient enrichment and culture system of PCSCs and identify the key genes involved in the regulation of PCSCs. The three-dimensional (3D) liquid suspension mammosphere culture system has been established for enrichment and culture of PCSCs in vitro as the cell spheres are likely to originate from individual cell clone, but it has been challenged because the cell spheroids could be a result of cell aggregation. Methods: We optimized the existing culture system by adding methylcellulose to create a 3D semi-solid system which prevented the non-specific aggregation. Then we identified the CSC properties of Panc-1 spheroid cells cultured by this system by detecting the genes associated with stemness and by evaluation of the tumorigenicity in vitro and in vivo through invasion, migration and xenograft experiments methods. Subsequently, we performed high-throughput sequencing (HTS) of the Panc-1 spheroid cells. Results: We confirmed the PCSCs properties and high malignancy of the Panc-1 spheroid cells enriched by our novel 3D semi-solid system both in vitro and in vivo. Hundreds of mRNA, microRNA (miRNA) and dozens of long non-coding RNA (LncRNA) were identified to be differentially regulated in PCSCs-like Panc-1 spheroid cells compared with their parental cells by HTS. Conclusions: Our results demonstrate an efficient enrichment and culture system for Panc-1 spheroid cells with the PCSCs properties. The differentially expressed genes and their targets identified by the HTS of the Panc-1 spheroid cells can serve as new potential biomarkers for pancreatic cancer diagnosis and targeted therapy.

scholarly journals Resistance of in Vitro-derived Cucumber Plants to Pythium aphanidermatum

HortScience ◽  
1990 ◽  
Vol 25 (8) ◽  
pp. 967-969 ◽  
Author(s):  
W. Msikita ◽  
H.T. Wilkinson ◽  
R.M. Skirvin
Keyword(s):  
Cucumis Sativus ◽  
Pythium Aphanidermatum ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Embryonic Axes ◽  
Rate Of Development ◽  
Primary Roots ◽  
Solid Agar ◽  
Semi Solid

`Embryonic axes-derived `Burpless Hybrid' cucumber (Cucumis sativus L.) plantlets germinated on Murashige and Skoog (MS) medium supplemented with 16 combinations of BAP and NAA and seedlings derived from whole seeds cultured on semi-solid agar were inoculated in vitro with two isolates (WFU3 and WFM13) of Pythium aphanidermatum. All axes-derived plantlets and whole seedlings inoculated with WFM13 isolates were susceptible to blight and died 2 days after inoculation. Similarly, all seedlings inoculated with WFU3 isolates were killed within 2 days after inoculation; however, the rate of development and severity of blight varied among the axes-derived plantlets. Blight on axes-derived plantlets, regenerated on MS medium supplemented with 2 mg BAP/liter and 0.2 mg NAA/liter, was significantly less than on regenerants cultured on all other amended MS media. On some media, callus developed on crowns and/or primary roots. The presence of callus influenced resistance to Pythium. In a second experiment, axes-derived cucumber regenerants from five genotypes, cultured on MS medium supplemented with 2 mg BAP/liter and 0.2 mg N&A/liter, were compared for their resistance to P. aphanidermatum isolate WFU3. Resistance was significantly greater for `Burpless Hybrid' and `Sweetslice' than for three other genotypes. Chemical names used: 6-benzylaminopurine (BAP); α -naphthaleneacetic acid (NAA).

scholarly journals Demonstration of burst-promoting activity of recombinant human GM-CSF on circulating erythroid progenitors using an assay involving the delayed addition of erythropoietin

Blood ◽  
1985 ◽  
Vol 66 (6) ◽  
pp. 1479-1481 ◽  
Author(s):  
RE Donahue ◽  
SG Emerson ◽  
EA Wang ◽  
GG Wong ◽  
SC Clark ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Peripheral Blood ◽  
Colony Formation ◽  
In Vitro Assay ◽  
Erythroid Progenitors ◽  
Vitro Assay ◽  
Gm Csf ◽  
Semi Solid

Abstract We demonstrate through the use of an in vitro assay involving the delayed addition of erythropoietin that human recombinant GM-CSF, cloned from a mature T cell line, Mo, clearly has burst-promoting activity (BPA) on peripheral blood erythroid progenitors at picomolar concentrations. Delay for up to 72 hours of the addition of erythropoietin to semi-solid methylcellulose cultures of concentrated peripheral blood progenitors minimizes or eliminates BPA-independent erythroid colony formation with little loss of BPA-dependent erythroid colony formation. This assay will prove useful in accurately detecting sources of BPA.

scholarly journals In Vitro Effect ofZingiber officinaleExtract on Growth ofStreptococcus mutansandStreptococcus sanguinis

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Arash Azizi ◽  
Shabnam Aghayan ◽  
Saeed Zaker ◽  
Mahdieh Shakeri ◽  
Navid Entezari ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Culture Medium ◽  
Zingiber Officinale ◽  
Bacterial Suspension ◽  
Tooth Decay ◽  
Agar Culture ◽  
Solid Agar ◽  
Vitro Effect ◽  
Serial Dilutions

Background and Objectives. Tooth decay is an infectious disease of microbial origin. Considering the increasing prevalence of antibiotic resistance due to their overuse and also their side effects, medicinal plants are now considered for use against bacterial infections. This study aimed to assess the effects of different concentrations ofZingiber officinaleextract on proliferation ofStreptococcus mutansandStreptococcus sanguinisin vitro.Materials and Methods. In this experimental study, serial dilutions of the extract were prepared in two sets of 10 test tubes for each bacterium (total of 20). Standard amounts of bacterial suspension were added; 100ƛof each tube was cultured on prepared solid agar plates and incubated at 37°C for 24 hours. Serial dilutions of the extract were prepared in another 20 tubes and 100ƛof each tube was added to blood agar culture medium while being prepared. The mixture was transferred to the plates. The bacteria were inoculated on plates and incubated as described.Results. The minimum inhibitory concentration (MIC) was 0.02 mg/mL forS. mutansand 0.3 mg/mL forS. sanguinis. The minimum bactericidal concentration (MBC) was 0.04 mg forS. mutansand 0.6 mg forS. sanguinis.Conclusion.Zingiber officinaleextract has significant antibacterial activity againstS. mutansandS. sanguiniscariogenic microorganisms.

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