Block in porcine gamete interaction by polyclonal antibodies to a pig ZP3β fragment having partial sequence homology to human ZP3

1993 ◽  
Vol 25 (3) ◽  
pp. 277-283 ◽  
Author(s):  
Harini Bagavant ◽  
E.C. Yurewicz ◽  
A.G. Sacco ◽  
G.P. Talwar ◽  
S.K. Gupta
Keyword(s):  
Sequence Homology ◽  
Polyclonal Antibodies ◽  
Partial Sequence ◽  
Gamete Interaction

Partial sequence homologies between cytoskeletal proteins, c-myc, Rous sarcoma virus and adenovirus proteins, transducin, and β- and γ-crystallins

1985 ◽  
Vol 5 (2) ◽  
pp. 167-174 ◽  
Author(s):  
M. James ◽  
C. Crabbe
Keyword(s):  
Sequence Homology ◽  
Rous Sarcoma Virus ◽  
Cytoskeletal Proteins ◽  
Partial Sequence ◽  
Virus Protein ◽  
Rous Sarcoma ◽  
Sequence Homologies ◽  
Significant Homology ◽  
Sarcoma Virus ◽  
Simian Sarcoma Virus

Computer based sequence comparisons indicate partial sequence homology between human c-myc, Rous sarcoma virus, adenovirus 7, and simian sarcoma virus proteins and the cytoskeletal proteins desmin, keratin and vimentin. In addition, sections of the oncogene proteins showed partial but significant homology to α and β subunits of transducin, γ-II and β-BP crystallins showed partial but significant homology to the cytoskeletal proteins keratin, vimentin, desmin, α and β-tubulin, and to adenovirus 7 and simian sarcoma virus transforming gene proteins. β-BP crystallin showed partial but significant homology to Rous sarcoma virus protein, and to α and y subunits of transducin. Both crystallins showed partial sequence homology to the GTP-binding protein elongation factor TU from Escherichia coli. These sequence homologies suggest a link between the mechanisms of normal lens cell differentiation, involving modifications to the cytoskeleton and subsequent changes to the pattern of protein synthesis, and mechanisms of neoplastic transformation. Furthermore the transducin-like region on β-crystallin may be important for its interaction with lens membranes and the maintenance of short-range order for lens transparency.

The carboxy-terminal peptides of 46 kDa and 300 kDa mannose 6-phosphate receptors share partial sequence homology and contain information for sorting in the early endosomal pathway

1997 ◽  
Vol 110 (8) ◽  
pp. 1023-1032 ◽  
Author(s):  
C. Boker ◽  
K. von Figura ◽  
A. Hille-Rehfeld
Keyword(s):  
Plasma Membrane ◽  
Sequence Homology ◽  
Cytoplasmic Domain ◽  
Endocytic Pathway ◽  
Partial Sequence ◽  
Semiquantitative Analysis ◽  
Intracellular Pool ◽  
Endosomal Pathway ◽  
Mannose 6 Phosphate ◽  
Carboxy Terminal

Recycling of mannose 6-phosphate receptors was investigated by microinjection of F(ab) fragments against their carboxy-terminal peptides (residues 54–67 or 150–164 of the cytoplasmic domain of 46 kDa and 300 kDa mannose 6-phosphate receptor, respectively). For each receptor, masking the carboxy-terminal peptide by the corresponding F(ab) fragments resulted in complete depletion of the intracellular pool. Redistributed 300 kDa mannose 6-phosphate receptor was shown to accumulate at the plasma membrane and to internalize anti-ectodomain antibodies. Internalization of anti-ectodomain antibodies was also observed for redistributed 46 kDa mannose 6-phosphate receptor. Semiquantitative analysis suggested that for both redistributed receptors the amount of intracellularly accumulated anti-ectodomain antibodies was reduced. In addition, downstream transport along the endosomal pathway was slowed down. These data suggest that sorting information for early steps in the endocytic pathway is contained within the carboxy-terminal peptides of mannose 6-phosphate receptors.

Biphenyl-associatedmeta-cleavage dioxygenases fromComamonas testosteroniB-356

1998 ◽  
Vol 44 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Paul Hein ◽  
Justin Powlowski ◽  
Diane Barriault ◽  
Yves Hurtubise ◽  
Darakshsan Ahmad ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Substrate Specificity ◽  
Enzyme Kinetics ◽  
Sequence Homology ◽  
Catalytic Properties ◽  
Partial Sequence ◽  
Comamonas Testosteroni ◽  
Catabolic Pathways

In addition to 2,3-dihydroxybiphenyl 1,2-dioxygenase (B1,2O), biphenyl-grown cells of Comamonas testosteroni B-356 were shown to produce a catechol 2,3-dioxygenase (C2,3O). B1,2O showed strong sequence homology with B1,2Os found in other biphenyl catabolic pathways, while partial sequence analysis of the C2,3O of B-356 suggested a relationship with xylEII-encoded C2,3O. The coexistence of two meta-cleavage dioxygenases in this strain prompted a comparison between the catalytic properties of the two enzymes. C2,3O has a much broader substrate specificity than native or His-tagged B1,2O: both enzymes were inhibited by chlorocatechols, but B1,2O was more sensitive than C2,3O. The results are discussed in terms of the physiological implications of interaction between metabolites from the lower biphenyl-chlorobiphenyl pathway and enzymes of the upper pathway.Key words: chlorobiphenyl, catabolism, dioxygenase, nucleotide sequence, enzyme kinetics.

scholarly journals Polyclonal Antibodies to Glutathione S-Transferase- Verotoxin Subunit A Fusion Proteins Neutralize Verotoxins

2002 ◽  
Vol 9 (3) ◽  
pp. 687-692 ◽  
Author(s):  
P. H. M. Leung ◽  
J. S. M. Peiris ◽  
W. W. S. Ng ◽  
W. C. Yam
Keyword(s):  
Escherichia Coli ◽  
Sequence Homology ◽  
Fusion Proteins ◽  
Polyclonal Antibodies ◽  
Glutathione S Transferase ◽  
Subunit A ◽  
Immunological Diagnosis ◽  
Cross Neutralization ◽  
Transmembrane Regions ◽  
Gst Fusion Proteins

ABSTRACT The A1 subunits of verotoxin-1 (VT1) and VT2 genes were cloned into pGEX-4T-2 for the expression of glutathione S-transferase (GST) fusion proteins. The N-terminal and the transmembrane regions of the A1 subunits were excluded from the constructs in order to increase the product yields. Polyclonal anti-VT1A1 and anti-VT2A1 antibodies were produced by immunizing rabbits with GST-VT1A1 and GST-VT2A1 fusion proteins, respectively. The antibodies were tested for their ability to neutralize active toxins from 45 VT-producing Escherichia coli (VTEC) strains. The antibodies had significantly high neutralizing activities against their homologous toxins. The average percentages of neutralization of VT1 by anti-GST-VT1A1 and anti-GST-VT2A1 were 76.7% ± 7.9% and 3.6% ± 2.3%, respectively, and those of VT2 were 1.7% ± 2.3% and 82.5% ± 13.9%, respectively. VT2 variant toxin was neutralized by anti-GST-VT2A1, with cross neutralization being a possible consequence of sequence homology between VT2 and a VT2 variant. To our knowledge, this is the first report on the production of polyclonal antibodies from GST-VT fusion proteins. The antibodies were shown to exhibit specific toxin neutralizing activities and may be useful for immunological diagnosis of VTEC infections.

Immunocytochemical methodology for the localization of neuronal antigens at the ultrastructural level

1990 ◽  
Vol 48 (3) ◽  
pp. 876-877
Author(s):  
Jorge Pecci Saavedra ◽  
Mark Connaughton ◽  
Juan José López ◽  
Alicia Brusco
Keyword(s):  
Spinal Cord ◽  
Substantia Nigra ◽  
Polyclonal Antibodies ◽  
Ultrastructural Level ◽  
Point Of View ◽  
Nerve Tissue ◽  
Tissue Fixation ◽  
Optimal Conditions ◽  
Interpeduncular Nucleus ◽  
Technical Point

The use of antibodies as labels for the localization of specific molecules in the nervous systan has been extensively applied in recent years. Both monoand polyclonal antibodies or antisera have been employed. The knowledge of the organization of neuronal connectivities, gliovascular relationships, glioneuronal relationships and other features of nerve tissue has greatly increased.A number of areas of the nervous systan have been analyzed in our laboratory, including the nuclei of the raphe system, the reticular formation, interpeduncular nucleus, substantia nigra, caudate nucleus, putamen, pallidum, spinal cord, pineal gland and others.From a technical point of view, a number of variables needed to be taken into account in order to obtain reliable and reproducible results. The design of the optimal conditions of tissue fixation, embedding, sectioning, dilution of antibodies, and adaptation of Sternberger PAP technique were sane of the parameters taken into account to optimize the results. It is critical that each step of the technique be defined for each particular case.

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