Cerebral glucose metabolic rates in normal human females versus normal males

1987 ◽  
Vol 21 (3) ◽  
pp. 237-245 ◽  
Author(s):  
Lewis R. Baxter ◽  
John C. Mazziotta ◽  
Michael E. Phelps ◽  
Carl E. Selin ◽  
Barry H. Guze ◽  
...  
Keyword(s):  
Metabolic Rates ◽  
Human Females ◽  
Normal Human ◽  
Normal Males

Circhoral fluctuations of serum total renin, inhibin and related hormones around the mid-cycle in normal human females

1992 ◽  
Vol 7 (3) ◽  
pp. 337-343 ◽  
Author(s):  
R. De Hertogh ◽  
L. Vankrieken ◽  
K. Thomas ◽  
M. de Gasparo
Keyword(s):  
Human Females ◽  
Normal Human

Basal prolactin and total lactogenic hormone levels by microbioassay and immunoassay in normal human sera

1991 ◽  
Vol 125 (6) ◽  
pp. 621-627 ◽  
Author(s):  
Paul R. Maddox ◽  
Derek L. Jones ◽  
Robert E. Mansel
Keyword(s):  
Human Serum ◽  
Significant Variation ◽  
Normal Human Serum ◽  
Hormone Levels ◽  
Menstrual Status ◽  
Lactogenic Hormone ◽  
Human Sera ◽  
Normal Human ◽  
Normal Males ◽  
Absolute Decrease

Abstract. The availability of an improved microbioassay for prolactin measurement has enabled comparison of lactogenic hormone bioactivity and immunoactivity in normal human serum. Serum was studied from 61 normal females and 15 normal males. The correlation of both assays was very close for all subjects with a mean ratio of bioassay to immunoassay of 1.5 (range 0.8-2.0) for prolactin and 1.4 (range 0.5-1.9) for total lactogenic hormone. There was no significant variation in prolactin or total lactogenic hormone values by microbioassay or immunoassay with sexual or menstrual status. Postmenopausal prolactin levels were lower by both assays compared with premenopausal values with a relative and absolute decrease in prolactin bioactivity with age. These findings indicate that there is a good correlation between prolactin bioactivity and immunoactivity in human serum.

Combined immunocytogenetic and molecular cytogenetic analysis of meiosis I oocytes from normal human females

Zygote ◽  
1998 ◽  
Vol 6 (1) ◽  
pp. 27-38 ◽  
Author(s):  
A.L. Barlow ◽  
M.A. Hultén
Keyword(s):  
Cytogenetic Analysis ◽  
Immune Serum ◽  
Lateral Element ◽  
Human Females ◽  
Molecular Cytogenetic ◽  
Human Centromere ◽  
Normal Human ◽  
Meiosis I

SummaryThe microspread oocytes of three fetuses, two of 16 weeks gestation and one of 15 weeks gestation, were labelled with a combination of anti-lateral element antiserum and a human centromere labelling auto-immune serum. The anti-lateral element serum was found to label both asynapsed axial elements and synapsed lateral elements strongly. Nuclei were found from leptotene to diplotene in all three fetuses. The use of the human auto-immune serum led to the observation of ‘staggered centromeres’ and ‘centromeric associations’ as well as tightly clustered centromeres in ‘stellar nuclei’. Nuclei displaying various aberrant features were detected. The use of antibody-labelled microspread oocytes as substrates for fluorescence in situ hybridisation (FISH) was found to be reliably successful only with repetitive (centromeric and telomeric) probes.

scholarly journals Parameters of Marrow Proliferative Capacity In Vitro: Detection of a Sex Difference in Normal Human Granulopoiesis

Blood ◽  
1974 ◽  
Vol 43 (6) ◽  
pp. 841-846 ◽  
Author(s):  
Alan L. Rosenblum ◽  
Joan M. Bull ◽  
Paul P. Carbone ◽  
Eleanor Stashick ◽  
Wilma M. K. McKoy
Keyword(s):  
Dose Response ◽  
Response Curve ◽  
Cell Concentration ◽  
Colony Growth ◽  
State Regulation ◽  
Proliferative Capacity ◽  
Normal Human ◽  
Males And Females ◽  
Normal Males

Abstract Sixteen bone marrow aspirates from normal males, cultured in vitro at various concentrations, were compared with ten aspirates from normal females. Marrow from males produced a significantly higher colony count than females when plated at a standard concentration of 2.0 x 105 cells per dish. This difference in colony-forming ability was found to be cell-dose related when dose-response studies for each marrow were obtained at concentrations of 1.0, 1.5, and 2.0 x 105 cells per plate. To more fully characterize marrow proliferative capacity in vitro, two additional parameters of growth were defined: (1) the slope of the dose-response curve and (2) the extrapolated cell concentration at "zero" colony growth. These parameters were found to differ significantly between male and female marrows and suggest that the mechanism of steady-state regulation of resting marrow may differ in males and females.

Ultrastructural modification of cellular interactions in cancer tumor

1978 ◽  
Vol 36 (2) ◽  
pp. 318-319
Author(s):  
N. P. Dmitrieva
Keyword(s):  
Cancer Cells ◽  
Human Thyroid ◽  
Adjacent Cell ◽  
Main Role ◽  
Cellular Interactions ◽  
Electron Microscopic ◽  
Cancer Tumor ◽  
Normal Human ◽  
Characteristic Features

One of the most characteristic features of cancer cells is their ability to metastasia. It is suggested that the modifications of the structure and properties of cancer cells surfaces play the main role in this process. The present work was aimed at finding out what ultrastructural features apear in tumor in vivo which removal of individual cancer cells from the cell population can provide. For this purpose the cellular interactions in the normal human thyroid and cancer tumor of this gland electron microscopic were studied. The tissues were fixed in osmium tetroxide and were embedded in Araldite-Epon.In normal human thyroid the most common type of intercellular contacts was represented by simple junction formed by the parallelalignment of adjacent cell membranees leaving in between an intermembranes space 15-20 nm filled with electronlucid material (Fig. 1a). Sometimes in the basal part of cells dilatations of the intercellular space 40-50 nm wide were found (Fig. 1a). Here the cell surfaces may form single short microvilli.

Scanning Microscopy of Human Intestinal Explants in Organ Culture

1972 ◽  
Vol 30 ◽  
pp. 396-397
Author(s):  
Bruce Wetzel ◽  
Robert Buscho ◽  
Raphael Dolin
Keyword(s):  
Electron Microscopy ◽  
Room Temperature ◽  
Culture Conditions ◽  
Human Fetuses ◽  
Enteric Disease ◽  
Organ Cultures ◽  
Growth Of A Variety ◽  
Normal Human ◽  
Fetal Intestine ◽  
Scanning Electron

It has been reported that explants of human fetal intestine can be maintained in culture for up to 21 days in a viable condition and that these organ cultures support the growth of a variety of known viral agents responsible for enteric disease. Scanning electron microscopy (SEM) has been undertaken on several series of these explants to determine their appearance under routine culture conditions.Fresh specimens of jejunum obtained from normal human fetuses were washed, dissected into l-4mm pieces, and cultured in modified Leibowitz L-15 medium at 34° C as previously described. Serial specimens were fixed each day in 3% glutaraldehyde for 90 minutes at room temperature, rinsed, dehydrated, and dried by the CO2 critical point method in a Denton DCP-1 device. Specimens were attached to aluminum stubs with 3M transfer tape No. 465, and one sample on each stub was carefully rolled along the adhesive such that villi were broken off to expose their interiors.

Human Stapes Crura: Normal Ultrastructure. Scanning Electron Microscopic Findings

1975 ◽  
Vol 33 ◽  
pp. 528-529
Author(s):  
M.D. Graham
Keyword(s):  
Electron Microscope ◽  
Surgical Treatment ◽  
Scanning Electron Microscope ◽  
Osmic Acid ◽  
Human Cadaver ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Normal Human ◽  
Microscopic Findings ◽  
Scanning Electron

The recent development of the scanning electron microscope has added great impetus to the study of ultrastructural details of normal human ossicles. A thorough description of the ultrastructure of the human ossicles is required in order to determine changes associated with disease processes following medical or surgical treatment.Human stapes crura were obtained at the time of surgery for clinical otosclerosis and from human cadaver material. The specimens to be examined by the scanning electron microscope were fixed immediately in the operating room in a cold phosphate buffered 2% gluteraldehyde solution, washed with Ringers, post fixed in cold 1% osmic acid and dehydrated in graded alcohol. Specimens were transferred from alcohol to a series of increasing concentrations of ethyl alcohol and amyl acetate. The tissue was then critical point dried, secured to aluminum stubs and coated with gold, approximately 150A thick on a rotating stage in a vacuum evaporator. The specimens were then studied with the Kent-Cambridge S4-10 Scanning Electron Microscope at an accelerating voltage of 20KV.

Albumin-Induced Endocytic Vacuoles in Tetrahymena Pyrijormis

1975 ◽  
Vol 33 ◽  
pp. 694-695
Author(s):  
L. J. Brenner ◽  
D. G. Osborne ◽  
B. L. Schumaker
Keyword(s):  
Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Protein Concentration ◽  
Tetrahymena Pyriformis ◽  
Sequence Of Events ◽  
Cytoplasmic Bodies ◽  
Food Vacuoles ◽  
Mouse Ascites ◽  
Normal Human

Exposure of the ciliate, Tetrahymena pyriformis, strain WH6, to normal human or rabbit sera or mouse ascites fluids induces the formation of large cytoplasmic bodies. By electron microscopy these (LB) are observed to be membrane-bounded structures, generally spherical and varying in size (Fig. 1), which do not resemble the food vacuoles of cells grown in proteinaceous broth. The possibility exists that the large bodies represent endocytic vacuoles containing material concentrated from the highly nutritive proteins and lipoproteins of the sera or ascites fluids. Tetrahymena mixed with bovine serum albumin or ovalbumin solutions having about the same protein concentration (7g/100 ml) as serum form endocytic vacuoles which bear little resemblance to the serum-induced LB. The albumin-induced structures (Fig. 2) are irregular in shape, rarely spherical, and have contents which vary in density and consistency. In this paper an attempt is made to formulate the sequence of events which might occur in the formation of the albumin-induced vacuoles.

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