Measuring cellular immune responses to malaria antigens in endemic populations: Epidemiological, parasitological and physiological factors which influence in vitro assays

1990 ◽  
Vol 25 (1-3) ◽  
pp. 221-229 ◽  
Author(s):  
Eleanor Riley ◽  
Brian Greenwood
Keyword(s):  
Immune Responses ◽  
In Vitro Assays ◽  
Physiological Factors ◽  
Cellular Immune Responses ◽  
Cellular Immune

scholarly journals Salmonella Adhesion, Invasion and Cellular Immune Responses Are Differentially Affected by Iron Concentrations in a Combined In Vitro Gut Fermentation-Cell Model

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e93549 ◽  
Author(s):  
Alexandra Dostal ◽  
Mélanie Gagnon ◽  
Christophe Chassard ◽  
Michael Bruce Zimmermann ◽  
Liam O'Mahony ◽  
...  
Keyword(s):  
Immune Responses ◽  
Cell Model ◽  
Cellular Immune Responses ◽  
Cellular Immune ◽  
Gut Fermentation ◽  
Iron Concentrations

scholarly journals A Combined Adjuvant TF–Al Consisting of TFPR1 and Aluminum Hydroxide Augments Strong Humoral and Cellular Immune Responses in Both C57BL/6 and BALB/c Mice

Vaccines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1408
Author(s):  
Qiao Li ◽  
Zhihua Liu ◽  
Yi Liu ◽  
Chen Liang ◽  
Jiayi Shu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Vaccine Development ◽  
Inbred Mouse ◽  
Effective Vaccine ◽  
Mouse Strains ◽  
Cellular Immune Responses ◽  
Recombinant Hbsag ◽  
Cellular Immune

TFPR1 is a novel adjuvant for protein and peptide antigens, which has been demonstrated in BALB/c mice in our previous studies; however, its adjuvanticity in mice with different genetic backgrounds remains unknown, and its adjuvanticity needs to be improved to fit the requirements for various vaccines. In this study, we first compared the adjuvanticity of TFPR1 in two commonly used inbred mouse strains, BALB/c and C57BL/6 mice, in vitro and in vivo, and demonstrated that TFPR1 activated TLR2 to exert its immune activity in vivo. Next, to prove the feasibility of TFPR1 acting as a major component of combined adjuvants, we prepared a combined adjuvant, TF–Al, by formulating TFPR1 and alum at a certain ratio and compared its adjuvanticity with that of TFPR1 and alum alone using OVA and recombinant HBsAg as model antigens in both BALB/c and C57BL/6 mice. Results showed that TFPR1 acts as an effective vaccine adjuvant in both BALB/c mice and C57BL/6 mice, and further demonstrated the role of TLR2 in the adjuvanticity of TFPR1 in vivo. In addition, we obtained a novel combined adjuvant, TF–Al, based on TFPR1, which can augment antibody and cellular immune responses in mice with different genetic backgrounds, suggesting its promise for vaccine development in the future.

Cellular immune responses to autologous chronic myelogenous leukaemia cells in vitro

1996 ◽  
Vol 42 (3) ◽  
pp. 193-199 ◽  
Author(s):  
Graham Pawelec ◽  
Arnika Rehbein ◽  
Elke Schlotz ◽  
Paul da Silva
Keyword(s):  
Immune Responses ◽  
Chronic Myelogenous Leukaemia ◽  
Myelogenous Leukaemia ◽  
Cellular Immune Responses ◽  
Cellular Immune

Inactivated Probiotic Bacteria Stimulate Cellular Immune Responses of Catla, Catla catla (Hamilton) In Vitro

2015 ◽  
Vol 7 (2) ◽  
pp. 101-106 ◽  
Author(s):  
Dibyendu Kamilya ◽  
Arunjyoti Baruah ◽  
Timothy Sangma ◽  
Supratim Chowdhury ◽  
Prasenjit Pal
Keyword(s):  
Immune Responses ◽  
Probiotic Bacteria ◽  
Catla Catla ◽  
Cellular Immune Responses ◽  
Cellular Immune

scholarly journals Cellular and Humoral Immunogenicity of a Candidate DNA Vaccine Expressing SARS-CoV-2 Spike Subunit 1

Vaccines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 852
Author(s):  
Khalid A. Alluhaybi ◽  
Rahaf H. Alharbi ◽  
Rowa Y. Alhabbab ◽  
Najwa D. Aljehani ◽  
Sawsan S. Alamri ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Neutralizing Antibodies ◽  
Vaccine Development ◽  
Cell Responses ◽  
Cellular Immune Responses ◽  
Specific Igg ◽  
Cellular Immune ◽  
Different Doses

The urgent need for effective, safe and equitably accessible vaccines to tackle the ongoing spread of COVID-19 led researchers to generate vaccine candidates targeting varieties of immunogens of SARS-CoV-2. Because of its crucial role in mediating binding and entry to host cell and its proven safety profile, the subunit 1 (S1) of the spike protein represents an attractive immunogen for vaccine development. Here, we developed and assessed the immunogenicity of a DNA vaccine encoding the SARS-CoV-2 S1. Following in vitro confirmation and characterization, the humoral and cellular immune responses of our vaccine candidate (pVAX-S1) was evaluated in BALB/c mice using two different doses, 25 µg and 50 µg. Our data showed high levels of SARS-CoV-2 specific IgG and neutralizing antibodies in mice immunized with three doses of pVAX-S1. Analysis of the induced IgG subclasses showed a Th1-polarized immune response, as demonstrated by the significant elevation of spike-specific IgG2a and IgG2b, compared to IgG1. Furthermore, we found that the immunization of mice with three doses of 50 µg of pVAX-S1 could elicit significant memory CD4+ and CD8+ T cell responses. Taken together, our data indicate that pVAX-S1 is immunogenic and safe in mice and is worthy of further preclinical and clinical evaluation.

scholarly journals Evaluation of the immune response to CRA and FRA recombinant antigens of Trypanosoma cruzi in C57BL/6 mice

2003 ◽  
Vol 36 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Valéria Rêgo Alves Pereira ◽  
Virginia Maria Barros de Lorena ◽  
Mineo Nakazawa ◽  
Ana Paula Galvão da Silva ◽  
Ulisses Montarroyos ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Immune Responses ◽  
Control Group ◽  
Mechanisms Of Resistance ◽  
Recombinant Antigens ◽  
Cellular Immune Responses ◽  
Cellular Immune ◽  
Antibody Levels ◽  
Cruzi Infection

Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Significant differences were observed between incorporation of [³H]- thymidine by spleen cell stimulated in vitro with CRA or FRA and the control group. These results suggest that CRA and FRA could be involved in mechanisms of resistance to Trypanosoma cruzi infection.

Mannan Antigen of Candida Albicans and Cellular Immune Responses in Vitro and in Vivo

1988 ◽  
pp. 185-196
Author(s):  
Anne Durandy ◽  
Alain Fischer ◽  
Edouard Drouhet ◽  
Claude Griscelli
Keyword(s):  
Candida Albicans ◽  
Immune Responses ◽  
Cellular Immune Responses ◽  
Cellular Immune
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