Epidermal growth factor attenuates cell proliferation by down-regulating the transforming growth factor-β receptor in the osteoblastic cell line MC3T3-E1

1991 ◽  
Vol 15 (2) ◽  
pp. 125-135 ◽  
Author(s):  
Hiroaki Jikihara ◽  
Hiromasa Ikegami ◽  
Masahiro Sakata ◽  
Ken-ichiro Morishige ◽  
Yoshiko Fujita ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Osteoblastic Cell ◽  
Osteoblastic Cell Line ◽  
Epidermal Growth ◽  
Β Receptor

Effects of Transforming Growth Factor-β1 on Decorin Expression by Undifferentiated Osteoblastic Cells

1997 ◽  
Vol 3 (S2) ◽  
pp. 187-188
Author(s):  
T. Yamada ◽  
N. Kubushiro ◽  
K. Shigemasa ◽  
T. Ikeda ◽  
M. Takagi
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Transforming Growth Factor ◽  
Bone Cells ◽  
Bone Matrix ◽  
Transforming Growth Factor Β ◽  
Osteoblastic Cells ◽  
Osteoblastic Cell ◽  
Experimental Conditions ◽  
Osteoblastic Cell Line

Decorin is the predominant proteoglycan isolated from bone of several animal species. Bone matrix decorin appears to bind transforming growth factor β (TGF-β) and enhances its bioactivity. TGF-β is stored in bone matrix in abundant amounts and modulates the synthesis of bone matrix proteins by osteoblasts. Thus it appears to play a role in regulation of bone formation during the bone remodeling process. The effect of TGF-β on decorin expression in bone cells has been evaluated in murine osteoblastic cells, but the results are divergent depending on the experimental conditions and cell types used. The present study investigated the effect of TGF-βl on the expression of decorin mRNA in two clonal rat osteoblastic cell lines with different stages of differentiation, ROS-C26 (C26) and ROS-C20 (C20); C26 is a potential osteoblast precursor cell line that is also capable of differentiating into muscle cells and adipocytes; C20 is a more differentiated osteoblastic cell line.

Differential effects of epidermal growth factor (EGF) on cell locomotion and cell proliferation in a cloned human embryonal carcinoma-derived cell line in vitro

1986 ◽  
Vol 86 (1) ◽  
pp. 47-55
Author(s):  
W. Engstrom
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Colony Diameter ◽  
Cell Locomotion ◽  
Epidermal Growth

The effects of epidermal growth factor (EGF) on clones from a human embryonal carcinoma-derived cell line (Tera-2) have been studied. Cells were plated at clonal densities, whereafter the effects of serum and EGF on cell locomotion and cell proliferation were examined. The addition of 50 ngEGF ml-1 resulted in increased migration, as judged by increased colony diameter in the presence of EGF. However, the effect of EGF on cell locomotion was rarely accompanied by any effect on cell proliferation. It was concluded that EGF exerts a preferential effect on cell migration in human embryonal carcinoma cells in vitro.

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