Effects of Transforming Growth Factor-β1 on Decorin Expression by Undifferentiated Osteoblastic Cells

1997 ◽  
Vol 3 (S2) ◽  
pp. 187-188
Author(s):  
T. Yamada ◽  
N. Kubushiro ◽  
K. Shigemasa ◽  
T. Ikeda ◽  
M. Takagi
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Transforming Growth Factor ◽  
Bone Cells ◽  
Bone Matrix ◽  
Transforming Growth Factor Β ◽  
Osteoblastic Cells ◽  
Osteoblastic Cell ◽  
Experimental Conditions ◽  
Osteoblastic Cell Line

Decorin is the predominant proteoglycan isolated from bone of several animal species. Bone matrix decorin appears to bind transforming growth factor β (TGF-β) and enhances its bioactivity. TGF-β is stored in bone matrix in abundant amounts and modulates the synthesis of bone matrix proteins by osteoblasts. Thus it appears to play a role in regulation of bone formation during the bone remodeling process. The effect of TGF-β on decorin expression in bone cells has been evaluated in murine osteoblastic cells, but the results are divergent depending on the experimental conditions and cell types used. The present study investigated the effect of TGF-βl on the expression of decorin mRNA in two clonal rat osteoblastic cell lines with different stages of differentiation, ROS-C26 (C26) and ROS-C20 (C20); C26 is a potential osteoblast precursor cell line that is also capable of differentiating into muscle cells and adipocytes; C20 is a more differentiated osteoblastic cell line.

scholarly journals Dexamethasone induces sodium-dependant vitamin C transporter in a mouse osteoblastic cell line MC3T3-E1

2001 ◽  
Vol 86 (2) ◽  
pp. 145-149 ◽  
Author(s):  
I. Fujita ◽  
J. Hirano ◽  
N. Itoh ◽  
T. Nakanishi ◽  
K. Tanaka
Keyword(s):  
Vitamin C ◽  
Cell Line ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Culture Conditions ◽  
Osteoblastic Cell ◽  
Prostaglandin E ◽  
Osteoblastic Cell Line ◽  
Cell Functions ◽  
Stimulation Of

The regulation of intracellular ascorbic acid (AsA) levels may be under the control of an AsA-specific membrane transporter. The present study investigates AsA uptake and expression of Na-dependent vitamin C transporter (SVCT) mRNA in the mouse osteoblastic cell line, MC3T3-E1. Among eight compounds tested, dexamethasone (Dex) all-trans retinoic acid, transforming growth factor β, prostaglandin E2 and transferrin significantly (P<0·01, P<0·01, P<0·05 and P<0·01 respectively) stimulated the update of AsA into MC3T3-E1 cells. Among these five, Dex was the most active, inducing mSVCT2 mRNA and the uptake of AsA in a time- and concentration-dependant manner. Dex did not induce mSVCT1 mRNA. These results suggest that the Dex-induced stimulation of AsA incorporation into osteoblastic cells is mediated by the induction of mSVCT2. Since Dex reduced alkaline phosphatase activity in MC3T3-E1 cells in our culture conditions, Dex-induced stimulation of AsA incorporation might not be the result of differentiation. Hormone-regulated changes of SVCT expression may have an important role in cell functions.

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