Attenuated transmissible gastroenteritis (TGE) virus useful as TGE vaccine prepared by mutagenesis, cultivation in pig cell culture and selection of protease-resistant strain

Vaccine ◽  
1990 ◽  
Vol 8 (1) ◽  
pp. 94
Keyword(s):  
Cell Culture ◽  
Resistant Strain ◽  
Transmissible Gastroenteritis ◽  
Selection Of

scholarly journals Selection of a New Highly Resistant Strain for Malolactic Fermentation under Difficult Conditions

2016 ◽  
Vol 30 (2) ◽  
Author(s):  
R. Guzzon ◽  
E. Poznanski ◽  
L. Conterno ◽  
P. Vagnoli ◽  
S. Krieger-Weber ◽  
...  
Keyword(s):  
Resistant Strain ◽  
Malolactic Fermentation ◽  
Selection Of

Selection of Medicinal Plants Suppressing Growth of Multidrug-Resistant Strain of Tuberculosis Causative Agent

2021 ◽  
pp. 2115-2118
Author(s):  
Irina Alexandrovna Ratnikova ◽  
Amankeldi Kurbanovich Sadanov ◽  
Nina Nicolaevna Gavrilova ◽  
Saltanat Emilkyzy Orazymbet ◽  
Raushan Zhumabekkyzy Kaptagai
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Medicinal Plants ◽  
Aqueous Extract ◽  
Causative Agent ◽  
Resistant Strain ◽  
Multidrug Resistant ◽  
Alcohol Extract ◽  
Selection Of ◽  
Multidrug Resistant Strain

The article describes selection of medicinal plants active against multidrug-resistant strain of tuberculosis causative agent. It has been discovered that all tested extracts of medicinal plants in 1:20 dilutions were active regarding multidrug-resistant strain of Mycobacterium tuberculosis T-320 except for hackberry aqueous extract. The most active was alcohol extract of parmelia, which completely suppressed growth of mycobacteria in 1:100 dilution on the 21st day of cultivation.

Phenolics from Cell Suspension Cultures of Penstemon serrulatus; Relation to Plant Organs

1997 ◽  
Vol 52 (7-8) ◽  
pp. 426-432 ◽  
Author(s):  
Zuzanna Skrzypek ◽  
Halina Wysokińska
Keyword(s):  
Cell Culture ◽  
Phenolic Compounds ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Naphthaleneacetic Acid ◽  
Phenolic Composition ◽  
Phenylpropanoid Glycosides ◽  
Selection Of

Abstract By repeated selection of pigment portions of tissue the red callus induced from root seed­lings of Penstemon serrulatus Menz. was chosen for suspension culture, which was maintained in Schenk and Hildebrandt medium supplemented with naphthaleneacetic acid (0.2 mg/l), 6-benzylaminopurine (2 mg/l) and sucrose (50 g/l). From the cultured cells eight phenolic compounds were isolated. They were identified as cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, luteolin, luteolin 7-O-glucoside, norartocarpetin 7-O-glucoside, verbascoside, martynoside and leucosceptoside A. The kind of cell line, its age and light irradiation were important factors in flavonoid production, but production of phenylpropanoid glycosides was found to be unaffected by these factors. The phenolic composition found in the cell culture was compared with those in the flowers and leaves of original plants of P. serrulatus.

scholarly journals Enhancement of Hepatitis C Virus RNA Replication by Cell Culture-Adaptive Mutations

2001 ◽  
Vol 75 (10) ◽  
pp. 4614-4624 ◽  
Author(s):  
Nicole Krieger ◽  
Volker Lohmann ◽  
Ralf Bartenschlager
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Colony Formation ◽  
Marker Gene ◽  
Rna Replication ◽  
Clear Correlation ◽  
Transfected Cells ◽  
Adaptive Mutations ◽  
Selection Of

ABSTRACT Studies of the Hepatitis C virus (HCV) replication cycle have been made possible with the development of subgenomic selectable RNAs that replicate autonomously in cultured cells. In these replicons the region encoding the HCV structural proteins was replaced by the neomycin phosphotransferase gene, allowing the selection of transfected cells that support high-level replication of these RNAs. Subsequent analyses revealed that, within selected cells, HCV RNAs had acquired adaptive mutations that increased the efficiency of colony formation by an unknown mechanism. Using a panel of replicons that differed in their degrees of cell culture adaptation, in this study we show that adaptive mutations enhance RNA replication. Transient-transfection assays that did not require selection of transfected cells demonstrated a clear correlation between the level of adaptation and RNA replication. The highest replication level was found with an adapted replicon carrying two amino acid substitutions located in NS3 and one in NS5A that acted synergistically. In contrast, the nonadapted RNA replicated only transiently and at a low level. The correlation between the efficiency of colony formation and RNA replication was corroborated with replicons in which the selectable marker gene was replaced by the gene encoding firefly luciferase. Upon transfection of naive Huh-7 cells, the levels of luciferase activity directly reflected the replication efficiencies of the various replicon RNAs. These results show that cell culture-adaptive mutations enhance HCV RNA replication.

P-201 Severe hypoxia promotes selection of repopulating progenitor cells in primary MDS bone marrow cell culture

2013 ◽  
Vol 37 ◽  
pp. S114
Author(s):  
E. Masala ◽  
F. Buchi ◽  
A. Valencia-Martinez ◽  
A. Gozzini ◽  
A. Sanna ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Severe Hypoxia ◽  
Bone Marrow Cell Culture ◽  
Selection Of
Sign in / Sign up

Export Citation Format

Share Document