Phenolics from Cell Suspension Cultures of Penstemon serrulatus; Relation to Plant Organs

1997 ◽  
Vol 52 (7-8) ◽  
pp. 426-432 ◽  
Author(s):  
Zuzanna Skrzypek ◽  
Halina Wysokińska
Keyword(s):  
Cell Culture ◽  
Phenolic Compounds ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Naphthaleneacetic Acid ◽  
Phenolic Composition ◽  
Phenylpropanoid Glycosides ◽  
Selection Of

Abstract By repeated selection of pigment portions of tissue the red callus induced from root seed­lings of Penstemon serrulatus Menz. was chosen for suspension culture, which was maintained in Schenk and Hildebrandt medium supplemented with naphthaleneacetic acid (0.2 mg/l), 6-benzylaminopurine (2 mg/l) and sucrose (50 g/l). From the cultured cells eight phenolic compounds were isolated. They were identified as cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, luteolin, luteolin 7-O-glucoside, norartocarpetin 7-O-glucoside, verbascoside, martynoside and leucosceptoside A. The kind of cell line, its age and light irradiation were important factors in flavonoid production, but production of phenylpropanoid glycosides was found to be unaffected by these factors. The phenolic composition found in the cell culture was compared with those in the flowers and leaves of original plants of P. serrulatus.

scholarly journals Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Culture by Oligosaccharide Elicitor from its Endophytic Fungus Fusarium oxysporum Dzf17

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401
Author(s):  
Ruifen Zhang ◽  
Peiqin Li ◽  
Lijian Xu ◽  
Yuanquan Chen ◽  
Peng Sui ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Fusarium Oxysporum ◽  
Cell Suspension Culture ◽  
Endophytic Fungus ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Fungal Cell ◽  
Partial Acid Hydrolysis ◽  
Dioscorea Zingiberensis

Diosgenin accumulation in cell suspension cultures of Dioscorea zingiberensis C. H. Wright was enhanced by treatment with saccharide elicitors from its endophytic fungus Fusarium oxysporum isolate Dzf17. The crude oligosaccharide was prepared by partial acid hydrolysis of the isolated Dzf17 fungal cell wall fragments. Optimal elicitation of diosgenin production by the isolated Dzf17 oligosaccharide in cell suspension culture was achieved when the oligosaccharide was added to the medium at a concentration of 30 mg/L after 16-day's continuous cell suspension culture, and the cells were cultured for another 8 days before harvesting. By using these optimal conditions, the diosgenin yield of the cultured cells reached its maximum of 5.25 mg/L, which was over a three-fold increase.

scholarly journals Elicitor-Induced Production of Biomass and Pharmaceutical Phenolic Compounds in Cell Suspension Culture of Date Palm (Phoenix dactylifera L.)

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4669
Author(s):  
Jameel Mohammed Al-Khayri ◽  
Poornananda Madhava Naik
Keyword(s):  
Cell Culture ◽  
Phenolic Compounds ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Caffeic Acid ◽  
Cell Suspension Culture ◽  
Date Palm ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Total Phenolic

Plants that synthesize bioactive compounds that have high antioxidant value and elicitation offer a reliable in vitro technique to produce important nutraceutical compounds. The objective of this study is to promote the biosynthesis of these phenolic compounds on a large scale using elicitors in date palm cell suspension culture. Elicitors such as pectin, yeast extract (YE), salicylic acid (SA), cadmium chloride (CdCl2), and silver nitrate (AgNO3) at 50, 100, and 200 mg/L concentrations are used. The effects of elicitors on cell culture were determined in terms of biomass [packed cell volume (PCV), fresh and dry weight], antioxidant activity, and phenolic compounds (catechin, caffeic acid, kaempferol, apigenin) were determined using high-performance liquid chromatography (HPLC). Results revealed that enhanced PCV (12.3%), total phenolic content [317.9 ± 28.7 mg gallic acid equivalents (GAE)/100 g of dry weight (DW)], and radical scavenging activity (86.0 ± 4.5%) were obtained in the 50 mg/L SA treated cell culture of Murashige and Skoog (MS) medium. The accumulation of optimum catechin (26.6 ± 1.3 µg/g DW), caffeic acid (31.4 ± 3.8 µg/g DW), and kaempferol (13.6 ± 1.6 µg/g DW) was found in the 50 mg/L SA-treated culture when compared to the control. These outcomes could be of great importance in the nutraceutical and agronomic industries.

Biosynthesis of phenolic compounds inVitis vinifera cell suspension cultures: Study on hydroxycinnamoyl CoA:ligase

1989 ◽  
Vol 8 (2) ◽  
pp. 93-96 ◽  
Author(s):  
Samia Lofty ◽  
Annie Fleuriet ◽  
Teresa Ramos ◽  
Jean-Jacques Macheix
Keyword(s):  
Phenolic Compounds ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

scholarly journals INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam.

2017 ◽  
Vol 16 (1) ◽  
pp. 1-11
Author(s):  
Aryani Leksonowati ◽  
Witjaksono Witjaksono ◽  
Diah Ratnadewi
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Formation ◽  
Combined Treatment ◽  
Cell Suspension Cultures ◽  
Compact Structure ◽  
Friable Callus ◽  
Aquilaria Malaccensis

Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1–2 mg/L 2.4-D and 0.2–0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27–32% callus coverage percentage.  The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 –1 mg/L 2,4-D.

Influence of Medium Composition on the Formation of Secondary Compounds in Cell Suspension Cultures of Catharanthus roseus (L.) G. Don

1980 ◽  
Vol 35 (7-8) ◽  
pp. 551-556 ◽  
Author(s):  
K.-H. Knobloch ◽  
J. Berlin
Keyword(s):  
Phenolic Compounds ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Culture Media ◽  
Sucrose Solution ◽  
Cell Suspension Cultures ◽  
Secondary Compounds ◽  
Suspension Cultures ◽  
Medium Composition ◽  
High Concentrations

Cell suspension cultures of Catharanthus roseus have been subjected to various media condi­tions in order to stimulate the formation of indole alkaloids. High ajmalicine contents (up to 0.5 mg/g cell fresh weight) were achieved by transferring 2-week-old cell suspensions to a 10-fold volume of a 8% sucrose solution. The alkaloid accumulation started two days after the transfer and reached a plateau after ten days. Furthermore an enhanced level of phenolic compounds was found, whereas growth of the culture was low. The accumulation of both, alkaloids and poly­phenols was stimulated by high concentrations of sucrose and low concentrations of nitrogen con­taining salts and phosphate. When these minerals were added to the sucrose solution in con­centrations commonly used for cell culture media, the accumulation of alkaloids and phenolic compounds was largely suppressed.

Indole Alkaloids from Cell Suspension Cultures of Stemmadenia tomentosa and Voacanga africana

1982 ◽  
Vol 37 (10) ◽  
pp. 857-860 ◽  
Author(s):  
Joachim Stöckigt ◽  
Karl-Heinz Pawelka ◽  
Ana Rother ◽  
Brigitte Deus
Keyword(s):  
Cell Culture ◽  
Cell Suspension ◽  
Growth Condition ◽  
Indole Alkaloids ◽  
Normal Growth ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Normal Growth Condition ◽  
Different Types

Abstract Cell suspension cultures of Stemmadenia tomentosa synthesized under normal growth condition the eight major indole alkaloids: (-)-tabersonine, (-)-minovincinine, (+)-conoflorine (voaphyl-line), condylocarpine, (+)-tubotaiwine (dihydrocondylocarpine), (-)-norfluorocurarine (vin-canine), (-)-vinervine, and (-)-coronaridine. These alkaloids consist of the three different types, Aspidosperma, Strychnos and Iboga. In contrast, cultures o f Voacanga africana produced mainly one alkaloid group (Aspidosperma-type) represented by (-)-tabersonine, lochnericine and (-)-minovincinine. Therefore this cell culture seems to be qualified for investigation concerning the biosynthesis of Aspidosperma alkaloids.

Elicitor-induced Changes of Enzyme Activities Related to Isoflavone and Pterocarpan Accumulation in Chickpea (Cicer arietinum L.) Cell Suspension Cultures

1988 ◽  
Vol 43 (7-8) ◽  
pp. 536-544 ◽  
Author(s):  
Susanne Daniel ◽  
Walter Hinderer ◽  
Wolfgang Barz
Keyword(s):  
Cell Culture ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Enzyme Activities ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Biochanin A ◽  
Primary Metabolism ◽  
Cicer Arietinum L ◽  
L Cell

The extractable activities of thirteen enzymes of primary and secondary metabolism have been measured in chickpea (Cicer arietinum L.) cell suspension cultures after treatment with an elicitor from the fungus Ascochyta rabiei (Pass.) Lab. The cell culture, derived from the A. rabiei resistant cultivar ILC 3279, constitutively accumulated the isoflavones biochanin A and formononetin together with their 7-O-glucosides and the 7-O-glucoside-6″-malonates. After elicitor application the cells rapidly form the pterocarpan phytoalexins medicarpin and maackiain. Among the enzymes of primary metabolism only the glucose 6-phosphate dehydrogenase exhibited a significant increase in activity with a maximum four hours after application of the elicitor. In phenylpropane metabolism the activities of phenylalanine ammonia lyase and chalcone synthase were enhanced by the elicitor and exhibited highest levels after four hours. In contrast the chalcone isomerase activity was not influenced by the elicitor. A substantial enhancement occurred with the isoflavone 7-O-glucosyltransferase activity eight hours after elicitor application. The results suggest that in this cell culture the elicitor-induced biosynthesis of pterocarpan phytoalexins was accompanied with a rapid and transient increase of those enzyme activities which are located at branching points of related pathways, i.e. pentose phosphate cycle, general phenylpropane metabolism, flavonoid formation and isoflavone conjugation.

Microtubule organization during the cell cycle of cultured Vicia hajastana Grossh.

1989 ◽  
Vol 67 (9) ◽  
pp. 545-552 ◽  
Author(s):  
D. H. Simmonds ◽  
E. Conibear ◽  
G. Setterfield
Keyword(s):  
Cell Cycle ◽  
Cell Culture ◽  
Cell Suspension ◽  
Plant Cell Culture ◽  
Cell Suspension Cultures ◽  
Cell Cortex ◽  
Hoechst 33258 ◽  
Microtubule Organization ◽  
Wide Range ◽  
Late Telophase

Microtubule (MT) organization was examined at each stage of the cell cycle in cell suspension cultures of Vicia hajastana Grossh. Simultaneous staining of MTs by immunofluorescence and DNA by Hoechst 33258, and microfluorimetric quantitation of DNA in interphase allowed direct correlation of MT configuration with mitotic stage and with G1, S, and G2 of interphase. The results indicated that in the majority of the cells the cortical MTs were disorganized in early G1, but then organized rapidly into parallel transverse arrays, remained ordered throughout S, and lost order in G2, probably near the onset of mitosis. A wide range of cell sizes were found in S, indicating that entry into S was loosely controlled. Preprophase bands, present in 55–80% of cells in prophase were observed in both disorganized cell clumps and regular cell files, indicating they were not exclusively associated with organized patterns of growth and division. In many cells, short MTs or MT clusters were observed in the cell cortex during all stages of mitosis. These MT remnants may serve as nucleating centres for new cortical MTs, which appear in late telophase as a disordered network. Ordering of MT occurs later in G1, indicating that MT nucleation and organization are two different processes.Key words: interphase, microfluorimetry, microtubules, mitosis, plant cell culture.

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