Differential expression of human placental growth hormone variant and chorionic somatomammotropin genes in choriocarcinoma cells treated with methotrexate

1993 ◽  
Vol 91 (1-2) ◽  
pp. 159-166 ◽  
Author(s):  
Barbara E. Nickel ◽  
Margaret E. Bock ◽  
Mark W. Nachtigal ◽  
Peter A. Cattini
Keyword(s):  
Growth Hormone ◽  
Differential Expression ◽  
Placental Growth Hormone ◽  
Chorionic Somatomammotropin ◽  
Choriocarcinoma Cells

scholarly journals Differential expression of human placental growth-hormone variant and chorionic somatomammotropin in culture

1990 ◽  
Vol 267 (3) ◽  
pp. 653-658 ◽  
Author(s):  
B E Nickel ◽  
E Kardami ◽  
P A Cattini
Keyword(s):  
Growth Hormone ◽  
Differential Expression ◽  
Sequence Similarity ◽  
Mrna Level ◽  
Proximal Region ◽  
Stable Gene ◽  
Rat Pituitary ◽  
Placental Growth Hormone ◽  
V Gene ◽  
Chorionic Somatomammotropin

Regulation of human placental growth-hormone variant (hGH-V) in the presence of its own promoter has been studied. At term, 10-20% of placental mRNA is specific for chorionic somatomammotropin (hCS-A and -B) compared with 0.05% hGH-V, yet these genes show more than 90% sequence similarity at the nucleotide level. By using stable gene transfer of intact hGH-V and hCS-A genes into rat pituitary (GC) cells, synthesis and release of hGH-V and hCS are detected. This suggests that hGH-V as well as hCS is secreted during pregnancy. The hCS-A mRNA level is higher than that observed from the hGH-V gene in stably transfected GC cells. Also, a hybrid gene containing hGH-V 5′-flanking DNA was less active than a hybrid hCS-A gene containing equivalent sequences after transient transfection of these cells. This correlates with the binding of a known transcription factor to a proximal region (-97/-66) of the hCS-A promoter, and not the equivalent hGH-V gene sequences. These results indicate that differential expression of hGH-V and hCS in GC cells is related, in part, to the strength of their respective promoters, and suggest a similar mechanism may exist in the placenta.

Human placental growth hormone

1997 ◽  
Vol 177 (6) ◽  
pp. 1526-1534 ◽  
Author(s):  
E. Alsat ◽  
J. Guibourdenche ◽  
D. Luton ◽  
F. Frankenne ◽  
D. Evain-Brion
Keyword(s):  
Growth Hormone ◽  
Placental Growth Hormone
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