Cold-induced activation of 1-aminocyclopropane-1-carboxylic acid metabolism in rewarmed ‘Granny Smith’ apples: Consequences on ripening

Changes in 1-aminocyclopropane-1-carboxylic acid metabolism in apples ( Malus domestica Borkh cv Granny Smith) were studied in relation to cold storage. Emphasis was given to the differential re sponsiveness of fruits to cold treatment as a function of stage of maturity at harvest. Fruits were held at 1 or 20 °C for 30 days, respectively, or exposed to 1 °C for 10 days and then storaged at 20 °C for up to 30 days. Fruits at 20 °C showed typical climacteric behavior. Differences at 1 °C between maturity stages in ethylene production and ACC oxidase activity were abolished, which showed that cold treatment is an important inducer of climacteric rise in preclimacteric Granny Smith apples. At 1 °C, ethylene production was lower than at 20 °C and the maxima in production were similar for all the stages of maturity, but took place at different times which corresponded exactly to the initial differ ences in harvest dates. After the transfer to 20 °C, fruits exhibited similar behavior as regards ethyl ene production, ACC oxidase activity, and ACC and MACC levels in relation to a harmonization process which is discussed in this study.

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A Seven-Gene Locus for Synthesis of Phenazine-1-Carboxylic Acid by Pseudomonas fluorescens2-79

Journal of Bacteriology ◽

10.1128/jb.180.9.2541-2548.1998 ◽

1998 ◽

Vol 180 (9) ◽

pp. 2541-2548 ◽

Cited By ~ 172

Author(s):

Dmitri V. Mavrodi ◽

Vladimir N. Ksenzenko ◽

Robert F. Bonsall ◽

R. James Cook ◽

Alexander M. Boronin ◽

...

Keyword(s):

Carboxylic Acid ◽

Gene Locus ◽

Acid Metabolism ◽

Shikimic Acid ◽

Expression System ◽

Multienzyme Complex ◽

Broad Spectrum Antibiotic ◽

T7 Promoter ◽

New Genes ◽

Chorismic Acid

ABSTRACT Pseudomonas fluorescens 2-79 produces the broad-spectrum antibiotic phenazine-1-carboxylic acid (PCA), which is active against a variety of fungal root pathogens. In this study, seven genes designated phzABCDEFG that are sufficient for synthesis of PCA were localized within a 6.8-kbBglII-XbaI fragment from the phenazine biosynthesis locus of strain 2-79. Polypeptides corresponding to allphz genes were identified by analysis of recombinant plasmids in a T7 promoter/polymerase expression system. Products of thephzC, phzD, and phzE genes have similarities to enzymes of shikimic acid and chorismic acid metabolism and, together with PhzF, are absolutely necessary for PCA production. PhzG is similar to pyridoxamine-5′-phosphate oxidases and probably is a source of cofactor for the PCA-synthesizing enzyme(s). Products of thephzA and phzB genes are highly homologous to each other and may be involved in stabilization of a putative PCA-synthesizing multienzyme complex. Two new genes, phzXand phzY, that are homologous to phzA andphzB, respectively, were cloned and sequenced from P. aureofaciens 30-84, which produces PCA, 2-hydroxyphenazine-1-carboxylic acid, and 2-hydroxyphenazine. Based on functional analysis of the phz genes from strains 2-79 and 30-84, we postulate that different species of fluorescent pseudomonads have similar genetic systems that confer the ability to synthesize PCA.

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Subcellular Localization of 1-Aminocyclopropane-1-Carboxylic Acid Metabolism in Plant Cells

Biochemical and Physiological Aspects of Ethylene Production in Lower and Higher Plants ◽

10.1007/978-94-009-1271-7_4 ◽

1989 ◽

pp. 33-40 ◽

Cited By ~ 1

Author(s):

Jean-Claude Pech ◽

Mondher Bouzayen ◽

Gilbert Alibert ◽

Alain Latché

Keyword(s):

Carboxylic Acid ◽

Subcellular Localization ◽

Acid Metabolism ◽

Plant Cells

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Hepatic Proteomic Analysis Reveals That Enhanced Carboxylic Acid Metabolism and Oxidoreduction Promote Muscle and Fat Deposition in Muscovy Duck

Animals ◽

10.3390/ani11082180 ◽

2021 ◽

Vol 11 (8) ◽

pp. 2180

Author(s):

Wanli Yang ◽

Xingyong Chen ◽

Congcong Wei ◽

Yutong Zhao ◽

Zhengquan Liu ◽

...

Keyword(s):

Carboxylic Acid ◽

Acid Metabolism ◽

Triosephosphate Isomerase ◽

Subcutaneous Fat ◽

Lipid Synthesis ◽

Reduction Process ◽

Fat Deposition ◽

Muscovy Duck ◽

Dependent Manner ◽

Muscovy Ducks

Liver is responsible for 90% of lipid synthesis in poultry; thus, it plays an important role in the growth of Muscovy ducks, which have a high fat deposition ability in a time-dependent manner. Therefore, male Muscovy ducks at 14, 28, 42, and 56 days were selected for body weight (BW), carcass weight (CW), subcutaneous fat thickness (SFT), abdominal fat weight (AFW), intramuscular fat content (IMF), and breast muscle fiber (BMF) diameter and density determination. Two-dimensional electrophoresis (2-DE) combining liquid chromatography linked to tandem mass spectrometry (LC-MS/MS) was used to analyze proteomic changes in liver at each stage. The BW, CW, AFW, SFT, and BMF diameter and density were significantly increased, while IMF content was significantly decreased at 28 to 42 days of age (p < 0.05). There were 57 differentially abundant protein (DEP) spots representing 40 proteins identified among the ages, in which 17, 41 and 4 spots were differentially abundant at 14 vs. 28, 28 vs. 42, and 42 vs. 56, respectively. Gene Ontology enrichment analysis found that DEPs were mostly enriched in the oxidation-reduction process, carboxylic acid metabolism, etc. Protein–protein interaction showed that catalase (CAT), triosephosphate isomerase (TPI), and protein disulfide-isomerase (PDI) were the key proteins responsible for the growth of Muscovy duck. In conclusion, 28 to 42 days of age is the crucial period for Muscovy ducks, and the ability of metabolism and antioxidants were significantly enhanced in liver.

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