Activity of Δ5-3β-Hydroxysteroid dehydrogenase, succinate dehydrogenase, and glucose-6-phosphate dehydrogenase in bovine luteinized follicular cysts and corpora lutea of estrous cycle: A quantitative histochemical study

1987 ◽  
Vol 15 (3-4) ◽  
pp. 145-159 ◽  
Author(s):  
A. Boos ◽  
I. Peukert-Adam ◽  
W. Meyer ◽  
R. Schwarz ◽  
E. Grunert
Keyword(s):  
Succinate Dehydrogenase ◽  
Estrous Cycle ◽  
Histochemical Study ◽  
Hydroxysteroid Dehydrogenase ◽  
Corpora Lutea ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Follicular Cysts

ACUTE ENZYME HISTOCHEMICAL CHANGES IN THE ZONA GLOMERULOSA OF THE RAT ADRENAL CORTEX

1968 ◽  
Vol 59 (3) ◽  
pp. 508-518
Author(s):  
J. D. Elema ◽  
M. J. Hardonk ◽  
Joh, Koudstaal ◽  
A. Arends
Keyword(s):  
Peritoneal Dialysis ◽  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Adrenal Cortex ◽  
Isocitrate Dehydrogenase ◽  
Hydroxysteroid Dehydrogenase ◽  
Zona Glomerulosa ◽  
Acute Changes ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Histochemical Changes

ABSTRACT Acute changes in glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase activity in the zona glomerulosa of the rat adrenal cortex were induced by peritoneal dialysis with 5 % glucose. Although less clear, the activity of 3β-ol-hydroxysteroid dehydrogenase also seemed to increase as well. No changes were seen in the activity of succinate dehydrogenase. Dialysis with 0.9 % NaCl had no effect on any of the enzymes investigated. The possible significance of these observations is discussed.

CYCLISCHE SCHWANKUNGEN DER AKTIVITÄTEN VON HYDROXYSTEROID-DEHYDROGENASEN IM CORPUS LUTEUM DES RINDES

1972 ◽  
Vol 69 (2) ◽  
pp. 369-384
Author(s):  
H. Brandau ◽  
L. Brandau ◽  
G. Mutzke
Keyword(s):  
Enzyme Activity ◽  
Corpus Luteum ◽  
Enzyme Activities ◽  
Optical Method ◽  
Activity Patterns ◽  
Hydroxysteroid Dehydrogenase ◽  
Corpora Lutea ◽  
Bovine Corpus Luteum ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACT In the bovine corpora lutea periodical activities of the Δ53β-, 3β-, 17β-and 20β-hydroxysteroid dehydrogenase (OHSDH) as well as activities of glyceraldehyde-3-phosphate- and glucose-6-phosphate dehydrogenase were measured quantitatively and the alterations throughout the different stages of the cycle were studied. After homogenization of the tissue and fractionate centrifugation the enzyme activities were determined by a standardized optical method. The activities of the Δ53β-, and 3β- and 17β-OHSDH increase slowly during the first 7 days of the cycle, the maximum is reached abruptly on the 12th to 13th day of the cycle. After a striking reduction the activities decline continually to the 19th to 21st day reaching the values detected at the beginning of the cycle. The 20β-OHSDH increases slowly to the maximum on the 15th day of the cycle. Activities of the 3α-OHSDH were obtained only inconsistently. The behaviour of the activities of G6PDH was nearly identical with that of the 3β-OHSDH, while the GAPDH shows only little fluctuations of its activities. The obtained enzyme activity patterns of the maturating and high functional corpus luteum correspond to the well-known data of the biosynthetic function of the bovine corpus luteum. The changes of the amounts of progesterone and 20β-progesterol agree with the course of the activities of the 3β- resp. 20β-OHSDH.

DEHYDROGENASES IN THE PREPUBERTAL RAT OVARY: A HISTOCHEMICAL STUDY OF Δ5-3β-HYDROXYSTEROID DEHYDROGENASE AND ENZYMES OF CARBOHYDRATE OXIDATION FOLLOWING OXYTHIAMINE TREATMENT

1973 ◽  
Vol 73 (4) ◽  
pp. 759-770 ◽  
Author(s):  
Jayasree Sen Gupta ◽  
Sudhansu K. Dey ◽  
C. Deb
Keyword(s):  
Histochemical Study ◽  
Succinic Dehydrogenase ◽  
Hydroxysteroid Dehydrogenase ◽  
Histochemical Demonstration ◽  
Pentose Phosphate ◽  
Diaphorase Activity ◽  
Rat Ovary ◽  
Prepubertal Rats ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Stimulation Of

ABSTRACT A histochemical study of the NAD- and NADP-linked dehydrogenases, Δ5-3β-hydroxysteroid dehydrogenase (Δ5-3β-ODH) and glucose-6-phosphate dehydrogenase (G-6-PD), and the corresponding tetrazolium reductases in prepubertal rat ovaries following the administration of oxythiamine HCl revealed diminution in the activities of the two dehydrogenases, while the NAD- and NADP-tetrazolium reductase activities remained unchanged. The same treatment resulted in stimulation of dihydrolipoic dehydrogenase (DLDH) and succinic dehydrogenase (SDH) activities in the ovarian tissues. Supplementary histochemical demonstration of Δ5-3β-OHD activity in phosphate buffer at pH 9.0 revealed a similar localization of the enzyme as that observed at pH 7.1 and which was free of the NAD-linked diaphorase activity. A diminution of this ovarian enzyme activity at this pH was also evident, following oxythiamine treatment. The results indicate a suppression of ovarian steroidogenesis in the prepubertal rats as a result of an alteration of the pentose phosphate pathway enzyme, G-6-PD, following treatment with oxythiamine HCl.

BIOCHEMICAL CHANGES IN THE CORPORA LUTEA OF PREGNANT AND LACTATING HAMSTERS

1978 ◽  
Vol 78 (2) ◽  
pp. 261-265 ◽  
Author(s):  
SUDHA CHATTERJEE ◽  
G. S. GREENWALD
Keyword(s):  
Alkaline Phosphatase ◽  
Lipid Metabolism ◽  
Cholesteryl Ester ◽  
Post Partum ◽  
Hydroxysteroid Dehydrogenase ◽  
Significant Decline ◽  
Corpora Lutea ◽  
Peripheral Plasma ◽  
Acid And Alkaline Phosphatase ◽  
Glucose 6 Phosphate Dehydrogenase

SUMMARY Progesterone was secreted by hamster corpora lutea between days 1 and 15 of pregnancy; on day 14, the concentration in the peripheral plasma reached a maximum. A pronounced fall in luteal weight began on the day after parturition and the corpora lutea had virtually disappeared by days 4–5 post partum. The concentration of protein and the weights of the corpora lutea reached peak levels on days 12 and 14 respectively. The highest levels of luteal RNA were reached on days 12–13. The concentration of DNA in the corpora lutea was constant throughout pregnancy, indicating that hypertrophy rather than hyperplasia accounted for the increase in luteal weight. Luteal concentrations of cholesterol and its esters were unchanged between days 1 and 14 of pregnancy except for a significant decline in the level of cholesteryl ester on days 8 and 13. During the final stages of regression of the corpora lutea during lactation, the luteal concentrations of free and esterified cholesterol rose to three and ten times respectively, the values obtained on day 2 of pregnancy. A significant increase in the concentration of acid and alkaline phosphatase began on day 16 of pregnancy correlated with the onset of structural luteolysis. The activity of luteal glucose-6-phosphate dehydrogenase peaked on days 2 (comparable to the value on day 2 of the oestrous cycle) and 13 of pregnancy and day 3 of lactation. The high levels on these days may be associated either with luteal steroidogenesis during pregnancy or with lipid metabolism during the post-partum involutional phase. The activity of luteal 3β-hydroxysteroid dehydrogenase was relatively constant except for significant decreases on days 10 and 13 of pregnancy. The results indicate that the activities of all of the biochemical variables in the corpora lutea of pregnancy were highest between days 11 and 13.

BIOCHEMICAL CHANGES IN THE CORPUS LUTEUM OF THE CYCLIC HAMSTER

1976 ◽  
Vol 68 (2) ◽  
pp. 251-256 ◽  
Author(s):  
SUDHA CHATTERJEE ◽  
G. S. GREENWALD
Keyword(s):  
Lipid Metabolism ◽  
Acid Phosphatase ◽  
Acid Phosphatase Activity ◽  
Hydroxysteroid Dehydrogenase ◽  
Corpora Lutea ◽  
Maximal Concentration ◽  
Gradual Decline ◽  
Cellular Hypertrophy ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Rapid Regression

SUMMARY The corpora lutea (CL) of the hamster secrete progesterone on days 1 and 2 of the oestrous cycle before marked regression at pro-oestrus (day 4) virtually eliminates them from the ovary. Luteal protein and RNA reached their maximum values on day 2 and declined up to day 4. DNA was constant throughout the cycle substantiating the importance of cellular hypertrophy rather than hyperplasia in the growth of the CL. Concentrations of cholesterol and its esters were unchanged between days 1 and 3 but almost doubled in concentration on day 4. Glucose-6-phosphate dehydrogenase activity was maximal on days 2 and 4, apparently associated on these days with steroidogenesis and lipid metabolism, respectively. A significant increase in acid phosphatase activity occurred on day 3 reaching maximal values on day 4, probably accounting for the rapid regression of the CL. The maximal concentration of 3β-hydroxysteroid dehydrogenase in CL was on day 1, with a gradual decline over the next 3 days.

Activity in the corpora lutea resulting from sterile matings in Clethrionomys glareolus: a histochemical study

1983 ◽  
Vol 99 (1) ◽  
pp. 9-NP
Author(s):  
L. M. Westlin ◽  
Monica Niklasson
Keyword(s):  
Enzyme Histochemistry ◽  
Histochemical Study ◽  
Hydroxysteroid Dehydrogenase ◽  
Clethrionomys Glareolus ◽  
Corpora Lutea ◽  
Steroid Production ◽  
Strong Reaction ◽  
Similar Activity ◽  
Bank Voles

The capacity for steroid production in the corpora lutea (CL) of bank voles was examined by enzyme histochemistry for 3β-hydroxysteroid dehydrogenase. The results revealed similar activity in the CL resulting from sterile matings and the CL of pregnancy 2 days after mating. Six days after mating, the CL of pregnancy showed a strong reaction, while there was almost no reaction in the CL from sterile matings, which were clearly regressed at this time. It is suggested that the CL from sterile matings produce progesterone before regression and that this progesterone may prime the luteotrophic complex, increasing fertility of subsequent matings.

ZUR BEDEUTUNG DES HYPOPHYSÄREN LUTEINISIERUNGSHORMONS FÜR SCHWANGERSCHAFT, GEBURT UND LACTATION BEI DER RATTE

1970 ◽  
Vol 65 (3_Suppl) ◽  
pp. S5-S32 ◽  
Author(s):  
K. Loewit
Keyword(s):  
Luteinizing Hormone ◽  
Early Pregnancy ◽  
Hydroxysteroid Dehydrogenase ◽  
The State ◽  
Termination Of Pregnancy ◽  
Progesterone Level ◽  
Corpora Lutea ◽  
Regulatory Properties ◽  
Duration Of Pregnancy

ABSTRACT The role of luteinizing hormone (LH) for the maintenance of pregnancy, parturition and lactation was investigated by immunological and histochemical methods in the rat. Neutralisation of endogenous rat-LH with Rabbit-Anti-Bovine-LH-Serum (selective hypophysectomy) from days 7-12 of pregnancy resulted in reabsorption of the foetuses and the reappearance of strong 20α-hydroxysteroid-dehydrogenase (20α-OHSD) activity in the corpora lutea (CL) of pregnancy, which normally show no such activity at that time. This effect could be prevented in part by concurrent pregnenolone administration and fully by progesterone, but was not influenced by oestrogen or prolactin. It is concluded that in early pregnancy LH is the main luteotrophic hormone in the rat even though prolactin might act synergistically with it. Antiserum treatment after the 12th day of gestation had no influence on the state or duration of pregnancy or on parturition. LH-injections during the first half of pregnancy had no luteolytic effects i. e. they did not activate 20α-OHSD activity. After day 16 they advanced the reappearance of the enzyme, but delayed parturition or resulted in stillbirths. Neither LH nor antiserum seemed to alter lactation. Since progesterone prevented both the termination of pregnancy and the recurrence of 20α-OHSD activity, it should have some regulatory properties on the enzyme. It is discussed whether the gonadotrophin-dependent progesterone level could regulate the 20α-OHSD activity rather than result from it.

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