In vitro antimicrobial susceptibility of glycopeptide-resistant enterococci

Abstract Purpose Cutibacterium acnes (C. acnes) is an emerging pathogen that is highly resistant to antibiotics and is capable of causing persistent infections that are difficult to treat. Methods & Materials Acne vulgaris patients visiting dermatology OPD of our tertiary care hospital during the study period of 2 months were recruited. Skin swabs were collected, and the sample was processed on 5% sheep-blood agar for anaerobic culture by the GasPak method. Isolates were identified by the standard biochemical test. Antimicrobial susceptibility testing was performed for clinically relevant antibiotics by the E-strip method. The clinical response was evaluated after 1-month follow-up to the prescribed antibiotics. Results Minocycline, doxycycline, ceftriaxone, and tetracycline were the most effective antibiotics. Nonsusceptibility to clindamycin and erythromycin were observed in 11.9% and 31% isolates, respectively, with 9.5% isolates being nonsusceptible to both. For none of the antibiotics we found significant difference in the proportion of susceptible and nonsusceptible isolates between mild, moderate, and severe grades of acne vulgaris. For none of the antibiotic regimens, significant difference was observed between nonresponders and responders. Twenty-seven patients received clindamycin and among them 16 of 19 responders and 6 of 8 nonresponders yielded growth of clindamycin-susceptible isolates (p = 0.57). Conclusion We observed significant prevalence of resistant strains of C. acnes among patients with acne vulgaris. No association was observed between in vitro susceptibility results and treatment outcome.

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Yersinia enterocolitica: In Vitro Antimicrobial Susceptibility

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.13.5.888 ◽

1978 ◽

Vol 13 (5) ◽

pp. 888-890 ◽

Cited By ~ 17

Author(s):

M. Raevuori ◽

S. M. Harvey ◽

M. J. Pickett ◽

W. J. Martin

Keyword(s):

Yersinia Enterocolitica ◽

Antimicrobial Susceptibility

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Etiology and in vitro antimicrobial susceptibility profile of strains isolated from bovine mastitis in dairy herds from the midwest region of Santa Catarina state, Brazil

Acta Veterinaria Brasilica ◽

10.21708/avb.2017.11.4.7266 ◽

2017 ◽

Vol 11 (4) ◽

pp. 219-225

Author(s):

Mylena Karoline Valmorbida ◽

Danielle Correia dos Santos Carneiro ◽

Keila Catarina Prior ◽

Eliete Griebeler ◽

Marcella Zampoli Troncarelli ◽

...

Keyword(s):

Antimicrobial Susceptibility ◽

Bovine Mastitis ◽

Dairy Herds ◽

Santa Catarina ◽

Antimicrobial Susceptibility Profile ◽

Midwest Region ◽

Santa Catarina State

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Gentamicin susceptibility among a sample of multi-drug resistantNeisseria gonorrhoeaeisolates in India

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01907-16 ◽

2016 ◽

pp. AAC.01907-16 ◽

Cited By ~ 1

Author(s):

Manju Bala ◽

Vikram Singh ◽

Aradhana Bhargava ◽

Monika Kakran ◽

Naveen Chandra Joshi ◽

...

Keyword(s):

Effective Treatment ◽

Treatment Outcomes ◽

Treatment Option ◽

Antimicrobial Susceptibility ◽

Susceptibility Testing ◽

Dual Therapy ◽

Clinical Treatment ◽

Antimicrobial Susceptibility Testing ◽

Effective Treatment Option

Antimicrobial susceptibility testing of 258N. gonorrhoeaeisolates by Etest determined that 60.1% were MDR while 5% strains had decreased susceptibility to currently recommended extended-spectrum cephalosporins (ESCs). Among these, 84.5% MDR and 76.9% strains having decreased susceptibility to ESCs were susceptible to gentamicin. No MDR isolate was resistant to gentamicin. Thesein vitroresults suggest that gentamicin might be an effective treatment option for the MDR strains and in dual therapy for gonorrhea. However, further research regarding the clinical treatment outcomes is essential.

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In vitro antimicrobial susceptibility of anaerobic bacteria isolated from caprine footrot

Acta Veterinaria Hungarica ◽

10.1556/avet.55.2007.1.2 ◽

2007 ◽

Vol 55 (1) ◽

pp. 11-20 ◽

Cited By ~ 2

Author(s):

Angela Lacombe-Antoneli ◽

S. Píriz ◽

S. Vadillo

Keyword(s):

Antibiotic Treatment ◽

Antimicrobial Susceptibility ◽

Antimicrobial Agents ◽

Anaerobic Bacteria ◽

Gram Negative ◽

Western Spain ◽

Clinical Cases

The in vitro antimicrobial susceptibility of Gram-negative anaerobic bacilli commonly isolated from footrot in goats was studied. A total of 97 isolates belonging to the genera Dichelobacter, Fusobacterium, Prevotella, Porphyromonas and Bacteroides, obtained from clinical cases of footrot in south-western Spain between March 2000 and May 2001, were tested against 25 antimicrobial agents comprising β-lactams, aminoglycosides, macrolides, chloramphenicol, quinolones, lincosamides, sulphonamides and tetracyclines in order to optimise antibiotic treatment of this disease in goats. β-lactams, tetracyclines and metronidazole displayed the highest in vitro efficacy against the species involved in the pathogenesis of footrot.

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In vitro studies of Dermatophilus congolensis antimicrobial susceptibility by determining minimal inhibitory and bacteriocidal concentrations

British Veterinary Journal ◽

10.1016/s0007-1935(05)80226-x ◽

1994 ◽

Vol 150 (2) ◽

pp. 189-196 ◽

Cited By ~ 6

Author(s):

J. Hermoso de Mendoza ◽

A. Arenas ◽

J. Rey ◽

J.M. Alonso ◽

M.C. Gil ◽

...

Keyword(s):

Antimicrobial Susceptibility ◽

In Vitro Studies

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In-vitro antimicrobial susceptibility and β-lactamases of ampicillin-resistant Escherichia coliin Hong Kong

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/34.1.65 ◽

1994 ◽

Vol 34 (1) ◽

pp. 65-71 ◽

Cited By ~ 2

Author(s):

T. K. W. Ling ◽

D. J. Lyon ◽

A. F. B. Cheng ◽

G. L. French

Keyword(s):

Hong Kong ◽

Antimicrobial Susceptibility

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Antimicrobial Susceptibility of Porphyromonas Gingivalis Strains Isolated From Periodontal Patients by Three Testing Protocols

10.21203/rs.3.rs-60211/v1 ◽

2020 ◽

Author(s):

Lorena Isbej ◽

Natacha Oyarzo ◽

María José Contreras ◽

Duniel Ortuño ◽

Marusella Lam ◽

...

Keyword(s):

Laboratory Test ◽

Porphyromonas Gingivalis ◽

Antimicrobial Susceptibility ◽

Randomized Clinical Trials ◽

Blood Agar ◽

Agar Dilution ◽

Susceptibility Tests ◽

High Level ◽

Testing Protocols

Abstract Background: The main goal of antimicrobials is to eliminate microorganisms that persists despite mechanical treatment. This is the case of Porphyromonas gingivalis (Pg), frequently isolated in patients with periodontitis. Global antibiotic studies evaluated in randomized clinical trials and in vitro studies have shown mixed results regarding effectiveness and susceptibility, even with different protocols where it is not clear if the laboratory test applied can affect the results. This information is relevant in order to obtain clinical outcomes and prevent antimicrobial resistance for their over-prescription or inadequate choice. The objective of this study was to describe the antimicrobial susceptibility in vitro of Pg to metronidazole, clindamycin, amoxicillin plus clavulanate, moxifloxacin and azithromycin in periodontal patients by three testing protocols.Methods: Microbiological samples were obtained in patients with a diagnosis of generalized moderate or severe periodontitis. They were incubated in anaerobic conditions for up to 7 days, and those morphologically compatible with Pg were isolated and identified by a mass spectrometer (MALDI-TOF MS). The three most frequently protocols for antimicrobials susceptibility tests (Blood agar- McFarland 0.5- Epsilometer test; Brucella blood agar- McFarland 1.0- Epsilometer test; Brucella blood agar- McFarland 0.5-Agar dilution) were applied to the same strain describing their profile and reporting any difference between the tests. The breakpoints considered the guidelines of CLSI and previous publications.Results: 50 patients (25 women, 25 men) with periodontitis between 34-69 years were selected. Finally, 25 Pg positives strains (50%) were recovered for the susceptibility analysis and all of them were highly sensitive to all antibiotics (range 96%-100%). Only one strain was resistant to azithromycin in one protocol, and no differences were found in the susceptibility results between the three tests.Conclusion: The Pg strains were highly susceptible to the five antibiotics evaluated in this population, showed a high level of susceptibility and significant agreement between the three tests applied, therefore the type of laboratory test used had not impacted on clinical interpretation. These findings are positives in terms of susceptibility and would provide several antibiotics treatment alternatives, and its prescription could be the best choice for the patient's specific context.

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Antimicrobial susceptibility pattern of Corynebacterium striatum.

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.40.11.2671 ◽

1996 ◽

Vol 40 (11) ◽

pp. 2671-2672 ◽

Cited By ~ 27

Author(s):

L Martínez-Martínez ◽

A Pascual ◽

K Bernard ◽

A I Suárez

Keyword(s):

Antimicrobial Susceptibility ◽

Antimicrobial Agents ◽

Susceptibility Pattern ◽

Beta Lactams ◽

Antimicrobial Susceptibility Pattern ◽

Mueller Hinton ◽

Corynebacterium Striatum ◽

Mueller Hinton Broth

The in vitro activities of 16 antimicrobial agents against 86 strains of Corynebacterium striatum were evaluated by microdilution using cation-adjusted Mueller-Hinton broth. MICs at which 90% of strains were inhibited were 0.06 microgram/ml for teicoplanin, 1 microgram/ml for vancomycin, 0.03 to 8 micrograms/ml for beta-lactams, 8 micrograms/ml for sparfloxacin, 16 micrograms/ml for ciprofloxacin, 16/304 micrograms/ml for co-trimoxazole (trimethoprim-sulfamethoxazole), 64 micrograms/ml for tetracycline, 128 micrograms/ml for gentamicin, and > 128 micrograms/ml for amikacin, erythromycin, and rifampin.

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