scholarly journals Etiology and in vitro antimicrobial susceptibility profile of strains isolated from bovine mastitis in dairy herds from the midwest region of Santa Catarina state, Brazil

2017 ◽  
Vol 11 (4) ◽  
pp. 219-225
Author(s):  
Mylena Karoline Valmorbida ◽  
Danielle Correia dos Santos Carneiro ◽  
Keila Catarina Prior ◽  
Eliete Griebeler ◽  
Marcella Zampoli Troncarelli ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Bovine Mastitis ◽  
Dairy Herds ◽  
Santa Catarina ◽  
Antimicrobial Susceptibility Profile ◽  
Midwest Region ◽  
Santa Catarina State

scholarly journals Training, development, and evaluation of on-farm culturing as a technical resource to reduce the use of antimicrobials in dairy herds in the Midwest Region of Santa Catarina State, Brazil

2022 ◽  
Vol 52 (1) ◽  
Author(s):  
Caroline Auer ◽  
Ana Flavia Begnini ◽  
Leonardo Leite Cardozo ◽  
Eliete Griebeler ◽  
Diogenes Dezen ◽  
...  
Keyword(s):  
Animal Health ◽  
Bovine Mastitis ◽  
Technical Training ◽  
Dairy Herds ◽  
Santa Catarina ◽  
Training Development ◽  
Technological Resource ◽  
Midwest Region ◽  
On Farm ◽  
Santa Catarina State

ABSTRACT: On-farm culturing is a technology booming in Brazil. It is based on the microbiological diagnosis of milk samples collected from clinical cases of bovine mastitis, on the farm where the animals are handled, quickly, simply, and at an affordable cost. With this resource, after 24 h of incubation, the isolated microorganisms are classified as gram positive or gram negative, helping to make decisions regarding the therapy of the animals. Considering the relevance of the Midwestern region of Santa Catarina State in the national dairy production scenario, the objectives of the study were: 1) to promote the technical training of the farmers in three dairy farms located in Treze Tílias-SC, 2) to assist in the implementation and evaluation of on-farm culturing as technological resource viewing the reduced use of antimicrobials in herds. In addition to the rapid acceptance by the farmers and their efficient training, with the use of the technology, there was a 45-50% reduction in the use of antimicrobials in dairy herds. This result demonstrated a significant effect on animal health, and determines an important cost reduction for farmers, as presented in detail in this manuscript.

scholarly journals Risk factors associated with the antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis

2014 ◽  
Vol 34 (10) ◽  
pp. 947-952 ◽  
Author(s):  
Daniele C. Beuron ◽  
Cristina S. Cortinhas ◽  
Bruno G. Botaro ◽  
Susana N. Macedo ◽  
Juliano L. Gonçalves ◽  
...  
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Management Practices ◽  
Bovine Mastitis ◽  
Factors Associated ◽  
Milk Samples ◽  
Treatment Procedures

The objective of this study was to evaluate herd management practices and mastitis treatment procedures as risk factors associated with Staphylococcus aureus antimicrobial resistance. For this study, 13 herds were selected to participate in the study to evaluate the association between their management practices and mastitis treatment procedures and in vitro antimicrobial susceptibility. A total of 1069 composite milk samples were collected aseptically from the selected cows in four different periods over two years. The samples were used for microbiological culturing of S. aureus isolates and evaluation of their antimicrobial susceptibility. A total of 756 samples (70.7%) were culture-positive, and S. aureus comprised 27.77% (n=210) of the isolates. The S. aureus isolates were tested using the disk-diffusion susceptibility assay with the following antimicrobials: ampicillin 10mg; clindamycin 2μg; penicillin 1mg; ceftiofur 30μg; gentamicin 10mg; sulfa-trimethoprim 25μg; enrofloxacin 5μg; sulfonamide 300μg; tetracycline 30μg; oxacillin 1mg; cephalothin 30μg and erythromycin 5μg. The variables that were significantly associated with S. aureus resistance were as follows: the treatment of clinical mastitis for ampicillin (OR=2.18), dry cow treatment for enrofloxacin (OR=2.11) and not sending milk samples for microbiological culture and susceptibility tests, for ampicillin (OR=2.57) and penicillin (OR=4.69). In conclusion, the identification of risk factors for S. aureus resistance against various mastitis antimicrobials is an important information that may help in practical recommendations for prudent use of antimicrobial in milk production.

Antimicrobial susceptibility profile in vitro of milk samples from cows from a small property in Guatapará, São Paulo State

2014 ◽  
Vol 8 (5) ◽  
Author(s):  
Rafael Akira Sato ◽  
Laryssa Freitas Ribeiro ◽  
Breno Vinicius de Lima ◽  
Rodrigo de Melo Magnabosco ◽  
Maria Imaculda Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
Milk Samples ◽  
Antimicrobial Susceptibility Profile ◽  
São Paulo State

scholarly journals Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2143
Author(s):  
Renata P. Santos ◽  
Fernando N. Souza ◽  
Ana Claudia D. Oliveira ◽  
Antônio F. de Souza Filho ◽  
Juliana Aizawa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Molecular Typing ◽  
Bovine Mastitis ◽  
Aureus Strain ◽  
Staphylococcal Protein A ◽  
Spa Typing ◽  
Intramammary Infections ◽  
Antimicrobial Susceptibility Profile

In the present study, we aimed to determine the antimicrobial resistance and molecular typing of Staphylococcus aureus recovered from transient and persistent intramammary infections and nares/muzzles in dairy cows. We investigated the antimicrobial resistance of 189 S. aureus strains using a broad antimicrobial susceptibility profile. Furthermore, 107 S. aureus isolates were strain-typed using staphylococcal protein-A (spa) typing. A large proportion of strains exhibited multidrug resistance to antimicrobials, including resistance to critically important antimicrobials, although no methicillin-resistant S. aureus strains were found. Our study did not strengthen the idea that extramammary niches (i.e., nares/muzzles) are an important source of S. aureus for bovine mastitis. A discrepancy in the antimicrobial resistance between S. aureus strains isolated from nares/muzzles and milk samples was observed. Furthermore, S. aureus isolates from transient and persistent intramammary infections (IMIs) did not differ by spa typing, suggesting that the persistence of bovine IMIs was determined by cow factors. Thus, the high level of multidrug-resistant S. aureus found in the two herds, considered together with the predominance of a well udder-adapted S. aureus strain, may contribute to our knowledge of the history of the high prevalence of mastitis caused by S. aureus, which is of great concern for animal and public health.

In vitro algicidal effect of polypyrrole on Prototheca species isolates from bovine mastitisAlgicidal activity of polypyrrole on Prototheca spp.

2020 ◽  
Vol 58 (8) ◽  
pp. 1114-1119
Author(s):  
Valessa Lunkes Ely ◽  
Mateus Matiuzzi da Costa ◽  
Helinando Pequeno de Oliveira ◽  
Fernando Antonio Gomes da Silva Júnior ◽  
Daniela Isabel Brayer Pereira ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Bovine Mastitis ◽  
Algicidal Activity ◽  
Dairy Herds ◽  
Morphological Alterations ◽  
Concentration Method ◽  
Genotype 2 ◽  
Algicidal Effect ◽  
Bovine Species

Abstract Algae of the genus Prototheca are microorganisms involved in the occurrence of diseases in humans and animals. In bovine species, Prototheca spp. cause environmental mastitis, productive losses in dairy herds, mainly leading to the discard of infected cows. Currently, there are no effective anti-Prototheca spp. drugs to combat this infection. Thus, the search for an efficacious therapy for Prototheca spp. infections have become essential. Highly soluble polypyrrole (Ppy) is a molecule with known antimicrobial activity. This study aimed to characterize Prototheca spp. isolates from bovine mastitis as well as to evaluate the susceptibility profile and to verify the morphological alterations on Prototheca spp. isolates treated with Ppy. In this research, 36 Brazilian isolates of Prototheca spp. were characterized by restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) assay for the mitochondrial cytB gene. Additionally, Ppy algicidal activity against these isolates of Prototheca spp. was assessed by minimal microbicidal concentration method in microplates. Further, scanning electron microscopy (SEM) was performed in order to verify the morphological alterations on Prototheca spp. isolates in response to Ppy. The isolates were characterized as belonging to Prototheca zopfii genotype 2 (35/36) and Prototheca blaschkeae (1/36). Ppy had an algicidal effect on all isolates tested at concentrations ranging from 15.625 μg ml−1 to 62.5 μg ml−1. SEM showed changes on planktonic and sessile P. zopfii, including a decrease of the number of cells with the presence of an amorphous substance involving the cells. The algicidal activity of Ppy suggests the therapeutic potential of this molecule in the prevention and treatment of Prototheca spp. in bovine mastitis.

Antimicrobial susceptibility profile in vitro samples of milk from cows mastitis from a dairy farm located in Jaboticabal, Sao Paulo State

2014 ◽  
Vol 8 (5) ◽  
Author(s):  
Laryssa Freitas Ribeiro ◽  
Rafael Akira Sato ◽  
Isabela Cristina Canavari ◽  
Carlos Eduardo Penner Belo ◽  
Gabriel Augusto Marques Rossi ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Dairy Farm ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
Antimicrobial Susceptibility Profile ◽  
São Paulo State

scholarly journals In vitro antimicrobial susceptibility of Staphylococcus aureus strains from dairy herds in KwaZulu-Natal

2011 ◽  
Vol 82 (2) ◽  
Author(s):  
T. Schmidt
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Bacterial Resistance ◽  
Bovine Mastitis ◽  
Dairy Industry ◽  
Economic Losses ◽  
Beta Lactam ◽  
Treatment Regimens ◽  
High Level

Staphylococcus aureus is 1 of the most important causes of bovine mastitis and is responsible for significant economic losses to the dairy industry worldwide. One of the principal approaches used in treating intramammary infections is the administration of antimicrobials. Due to the propensity of S. aureus to develop resistance, antimicrobial susceptibility monitoring is necessary to ensure that treatment regimens are effective. As part of this investigation, 90 S. aureus strains isolated from mastitis cases submitted to Allerton Provincial Veterinary Laboratory during 2008 and 2009 were evaluated for their susceptibility to a panel of 10 antimicrobials. Only 8 of the 90 S. aureus isolates tested (8.9 %) were found to be susceptible to all of the antimicrobials evaluated. A very high level of resistance to the beta-lactam antibiotics was noted: 47.8 % of the isolates were resistant to penicillin and 65.6 % were resistant to ampicillin. Minimal resistance to oxacillin, cephalothin and trimethoprim-sulfamethoxazole (1.1 %) was found. Seventeen (18.9 %) of the isolates tested were found to be resistant to 3 or more antimicrobials. The need for vigilant monitoring of bacterial resistance trends in the dairy industry is warranted as the potential public health implications are significant.

scholarly journals Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples

2020 ◽  
Author(s):  
Renata P. Santos ◽  
Fernando N. Souza ◽  
Ana Claudia C. Oliveira ◽  
Antônio F. de Souza Filho ◽  
Juliana Aizawa ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Protein A ◽  
Bovine Mastitis ◽  
Multidrug Resistant ◽  
Aureus Strain ◽  
Staphylococcal Protein A ◽  
Spa Typing ◽  
Antimicrobial Susceptibility Profile ◽  
High Level

In the present study, we aimed to determine the antimicrobial resistance and genetic structure of a population of S. aureus recovered from transient and persistent intramammary infections and nares/muzzles. We investigated the antimicrobial resistance of 189 S. aureus strains using a broad antimicrobial susceptibility profile. Furthermore, 107 S. aureus isolates were strain-typed using staphylococcal protein-A (spa) typing. Here, a great proportion of strains exhibited multidrug resistance to antimicrobials, including resistance to critically important antimicrobials, although no methicillin-resistant S. aureus strains were found. Our study did not strengthen the idea that extramammary niches (i.e., nares/muzzles) are an important source for S. aureus. A discrepancy in the antimicrobial resistance between S. aureus strains isolated from nasal/muzzles and milk samples was observed. Furthermore, S. aureus isolates from transient and persistent IMIs did not differ by spa typing, suggesting that the persistence of bovine IMIs was determined by cow factors. Thus, the high level of multidrug-resistant S. aureus found in the two herds studied together with the predominance of a well udder-adapted S. aureus strain may contribute to the history of the high prevalence of mastitis caused by S. aureus, leading to great animal and public health concerns.

scholarly journals Bovine mastitis: prevalence and antimicrobial susceptibility profile and detection of genes associated with biofilm formation in Staphylococcus aureus

2016 ◽  
Vol 37 (3) ◽  
pp. 1369
Author(s):  
Valeska Paula Casanova ◽  
Juceam Appio ◽  
Eduardo Kohl ◽  
Tais Regina Michaelsen ◽  
Daniel Santos Paim ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Bovine Mastitis ◽  
Antimicrobial Susceptibility Profile ◽  
Nocardia Sp ◽  
Polymerase Chain ◽  
Safe Food ◽  
Corynebacterium Sp

Brazil currently ranks as one of the world leaders in food production and exportation. This scenario encourages the development of animal and plant health programs to ensure the production of safe food, helping the country to become an international provider of food for excellence. However, some health problems in dairy production, such as mastitis, have garnered increasing concern. This study aimed to estimate the prevalence of bovine mastitis in select properties located in the western Santa Catarina region, to assess the susceptibility profile to antimicrobial agents used for treatment and to check for the presence of genes (icaA and icaD) associated with biofilm formation in Staphylococcus aureus. In 148 milk samples collected, 72.97% had bacterial growth (n = 108). Among the isolated microorganisms, 21.62% (n = 32) were classified as Staphylococcus aureus, 18.91% (n = 28) as Staphylococcus sp. coagulase negative, 7.43% (n = 11) as Corynebacterium sp., 6.76% (n = 10) as Staphylococcus sp. coagulase positive, 5.41% (n = 8) as Nocardia sp. and 12.83% (n = 19) classified in different bacterial genera. Among the isolates submitted for antimicrobial susceptibility testing, it was observed that 8.95% (n = 6/67) had resistance to amoxicillin, 8.04% (n = 7/87) to ampicillin, 5.88% (n = 5/85) to cephalothin, 3.40% (n = 3/88) to ceftiofur and enrofloxacin, 20.45% (n = 18/88) to streptomycin, 17.04% (n = 15/88) to gentamicin and lincomycin, 31.81% (n = 28/88) to neomycin, 14.94% (n = 13/87) to penicillin and 25% (n = 22/88) to tetracycline. Staphylococcus sp. coagulase negative isolates showed higher multidrug resistance when compared to those of S. aureus and Staphylococcus sp. coagulase positive. Thirty-one strains of S. aureus isolates were genotypically tested by polymerase chain reaction (PCR), yielding a positive result for the icaA gene in 83.87% of the samples, 80.64% positive for icaD and 74.19% of these showed both genes. The results reinforce the importance of controlling this disease by adopting strict hygiene protocols and disinfection of equipment used for milking and the prescription of appropriate therapies to clinical and subclinical cases of mastitis, as well as evaluating not only the susceptibility profile of isolates but also the biofilm formation capacity.

Sign in / Sign up

Export Citation Format

Share Document