An electron microscopic study of cauliflower mosaic virus-induced viroplasms: Unusual structures within the viroplasm matrix with possible functional significance in the viral replication cycle

1988 ◽  
Vol 100 (2) ◽  
pp. 118-125 ◽  
Author(s):  
D. Rodríguez ◽  
D. López-Abella ◽  
J.R. Díaz-Ruiz
Keyword(s):  
Viral Replication ◽  
Mosaic Virus ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Functional Significance ◽  
Replication Cycle ◽  
Cauliflower Mosaic Virus ◽  
Electron Microscopic

The Brindley's glands of Rhodnius prolixus. I. Structure of the mature gland

1979 ◽  
Vol 57 (5) ◽  
pp. 1109-1119 ◽  
Author(s):  
F. M. Barrett ◽  
B. F. Millen ◽  
J. Lai-Fook
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Functional Significance ◽  
Type A ◽  
Rhodnius Prolixus ◽  
Type B ◽  
Electron Microscopic ◽  
Dermal Glands ◽  
Similarities And Differences ◽  
Secretory Apparatus

A detailed light and electron microscopic study of the Brindley's glands of adult Rhodnius prolixus has confirmed the presence of type B glandular units similar to those described for the dermal glands of Rhodnius and has identified the second type A glandular unit, also composed of secretory apparatus, saccule, and duct, but outnumbered approximately 18:1 by the type B units. Similarities and differences between type A and type B glandular units are described and the possible functional significance of the two types of unit is discussed.

AN ELECTRON MICROSCOPIC STUDY OF THE PARATHYROID GLANDS IN MAN: EVIDENCE OF SECRETORY MATERIAL

1969 ◽  
Vol 61 (2) ◽  
pp. 334-342 ◽  
Author(s):  
Richard J. Weymouth ◽  
Hugo R. Seibel
Keyword(s):  
Endothelial Cells ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Functional Significance ◽  
Metabolic Process ◽  
Parathyroid Glands ◽  
Secretory Material ◽  
Electron Microscopic ◽  
Dense Bodies ◽  
Transitional Cells

ABSTRACT The ultrastructure of one hyperplastic and three adenomatous parathyroid glands have been described. Electron dense bodies measuring 0.08–0.5 microns in diameter and similar to those reported by Roth & Munger (1962) and Weymouth & Sheridan (1966) were described. These bodies were increased in number in the pathological glands, observed primarily at the vascular pole of the cell and frequently within the adjoining endothelial cells. Similar structures were found frequently within the mitochondria. In all probability, these electron dense bodies represent secretory material; the mitochondria play an intimate role in this metabolic process of secretion. Large, whorl-shaped structures, composed of concentric membranes, were also described. These structures may be of functional significance in hypersecreting glands, since they were observed in the pathological parathyroid glands only. »Transitional« cells were readily apparent.

The Growth of Herpesvirus mulatto in Human Fibroblast

1974 ◽  
Vol 32 ◽  
pp. 244-245
Author(s):  
Glennelle Washington ◽  
Philip P. McGrath ◽  
Peter R. Graze ◽  
Ivor Royston
Keyword(s):  
Rhesus Monkey ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Peripheral Blood ◽  
Human Fibroblast ◽  
Rhesus Monkeys ◽  
Human Fibroblasts ◽  
Electron Microscopic ◽  
Specific Antisera ◽  
Peripheral Blood Leucocytes

Herpes-like viruses were isolated from rhesus monkey peripheral blood leucocytes when co-cultivated with WI-38 cells. The virus was originally designated rhesus leucocyte-associated herpesvirus (LAHV) and subsequently called Herpesvirus mulatta (HVM). The original isolations were from juvenile rhesus monkeys shown to be free of antibody to rhesus cytomegalic virus. The virus could only be propagated in human or simian fibroblasts. Use of specific antisera developed from HVM showed no relationship between this virus and other herpesviruses. An electron microscopic study was undertaken to determine the morphology of Herpesvirus mulatta (HVM) in infected human fibroblasts.

Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

1971 ◽  
Vol 29 ◽  
pp. 338-339
Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall
Keyword(s):  
Cell Wall ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Genetic Heterogeneity ◽  
Morphological Characteristics ◽  
Electron Microscopic ◽  
Dna Reassociation ◽  
Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

A New Technique for the Light and Electron Microscopic Study of Individual Cerebro-Spinal Fluid Cells in a Mona Plane Layer

1976 ◽  
Vol 34 ◽  
pp. 362-363
Author(s):  
L.A. Dell
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Plane Layer ◽  
Ultrastructural Level ◽  
Spinal Fluid ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Easy Method ◽  
Cell Pellet ◽  
A New Technique

A new method has been developed which readily offers the microscopist a possibility for both light and electron microscopic study of selected cells from the cerebrospinal fluid. Previous attempts to examine these cells in the spinal fluid at the ultrastructural level were based on modifications of cell pellet techniques developed for peripheral blood. These earlier methods were limited in application by the number of cells in spinal fluid required to obtain a sufficient size pellet and by the lack of an easy method of cellular identification between the light and electron microscopic level. The newly developed method routinely employs microscope slides coated with Siliclad and tungsten oxide for duplicate cytocentrifuge preparations of diagnostic spinal fluid specimens. Work done by Kushida and Suzuki provided a basis for our use of the metal oxide.

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