Different effects of thiol and nonthiol ace inhibitors on copper-induced lipid and protein oxidative modification

Background. Cardiovascular morbidity is a topical issue; stress is an essential contributing factor. Pathogenic links in damaging stress impact on the animal units of different reactivity is promising in disease prevention and development of individual correction methods.Objective. Stress-induced development of cardiovascular pathology is undeniable, the stress impact depending on individual systemic response, age and sex.Methods. 96 hypoxia high- and low-resistant (HR and LR, respectively) Wistar rats aged 5.5-6 months were used in experiments. Changes of lipid peroxidation processes, as well as protein oxidative modification, nitrite anion content and the indices of antioxidant defence under immobilizing stress were studied in the research. Results. Immobilizing stress causes the development of oxidative and carbonyl stress in HR and LR rats that is more pronounced in LR group; and the activation of antioxidant defence system. In males, stress development is concomitant with increased catalase activity as well as that of blood peroxidase, ceruloplasmin and reduced glutathione content, whereas increased catalase and ceruloplasmin activity has been found in HR females, and that of superoxide dismutase and ceruloplasmin – in LR group. As compared with the females, more intensive oxidative and nitroxidative stress, protein oxidative modification, and stress-related accumulation of circulating immune complexes have been found in the males.Conclusion. The most intensive oxidative and nitroxidative stress, protein oxidative modification, and stress-related accumulation of circulating immune complexes have been found in the hypoxia low-resistant males.

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Protein oxidative modification and cathepsin H activity in rats’ thymocytes at nitrogen oxide (II) synthesis modulation in vitro

Kazan medical journal ◽

10.17816/kmj1842 ◽

2014 ◽

Vol 95 (4) ◽

pp. 553-557 ◽

Cited By ~ 2

Author(s):

Yu V Abalenikhina ◽

M A Fomina

Keyword(s):

Methyl Ester ◽

Nitrogen Oxide ◽

Nutrient Medium ◽

No Synthase ◽

Oxidative Modification ◽

Control Group ◽

Griess Reagent ◽

Protein Oxidative Modification ◽

Cathepsin H

Aim. To study the influence of substrate for nitrogen oxide (II) synthesis - L-arginine - and non-selective NO-synthase inhibitor - N-nitro-L-arginine-methyl ester - on protein oxidative modification in combination with rats’ thymocytes cathepsin H activity estimation in vitro. Methods. The study was performed on male Wistar rats with body weight of 280-320 g. Freshly-separated thymocytes were incubated in vitro in the full nutrient medium containing 5 мМ of N-nitro-L-arginine-methyl ester (n=8) or 5 мМ of L-arginine for 24 hours at the temperature of 37 °C (n=8). Control group consisted of thymocytes incubated in the same conditions in the full nutrient medium (n=8) for 24 hours. Nitric oxide metabolites levels were measured by spectrophotometry in the visible spectrum using the reaction with Griess reagent. Cathepsin H activity was estimated by BarrettKirschke spectrofluorimetry. Protein oxidative modification was measured by R.L. Levine method in E.E. Dubinina modification followed by carbonyl derivatives absorption spectrum quantitative analysis. Results. In nitrogen oxide (II) synthesis deficiency model, protein oxidative modification degree increased, mainly due to basic and neutral aldehyde- and ketone-dinitrophenylhydrazones level increase. Those changes were accompanied by increased activity of cathepsin H. In nitrogen oxide (II) synthesis stimulation model, level of oxidative-modified proteins decreased, mainly due to lower levels of neutral amino acid derivatives, cathepsin H activity didn’t change. Conclusion. In vitro nonselective inhibitor of inducible NO-synthase - N-nitro-L-arginine-methyl ester - stimulates protein oxidative modification and increases activity of cathepsin Н; substrate of NO synthesis - L-arginine - showes antioxidant effect.

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Prognostic factors for anti-cancer therapy efficiency

Kazan medical journal ◽

10.17816/kmj1919 ◽

2013 ◽

Vol 94 (5) ◽

pp. 681-685

Author(s):

E I Erlykina ◽

T V Kopytova ◽

A V Alyasova ◽

I G Terentiev ◽

T N Gorshkova ◽

...

Keyword(s):

Colorectal Cancer ◽

Free Radical ◽

Blood Plasma ◽

Gamma Globulin ◽

Oxidative Modification ◽

Cancer Relapse ◽

Protein Oxidative Modification ◽

Endogenous Intoxication ◽

Urine Bladder ◽

Free Radical Activity

Aim. To investigate the relationship between blood plasma biochemical indicators in patients with solid tumors before a treatment and after the first course of chemotherapy with objective treatment response. Methods. Blood plasma samples taken from 14 patients with cancer relapse were studied before the treatment and after the first course of specific chemotherapy (carboplatin, methotrexate, vinblastine in patients with urine bladder cancer and irinotecan, leukovorin and fluorouracil in patients with colorectal cancer). The first group included patients with colorectal cancer relapse (males - 3, females - 4) aged 57-62 years. The second group included patients with urine bladder cancer relapse (males - 5, females - 2) aged 48-64 years. Free radical activity and protein oxidative modification, as well as endogenous intoxication and major mineral and trace elements levels were studied. Results. When achieving the objective chemotherapy effect in patients after the first treatment course, an increase of alpha-1 globulin and gamma globulin level, as well as an increase of phosphorus, zinc, lithium and iron blood plasma level was observed. In case of following disease progression, the opposite dynamics of above-mentioned indicators was revealed. In patients with good objective effect of polychemotherapy after the first treatment course, a significant increase of endogenous intoxication and blood plasma free radical activity decrease were observed. If the following tumor progression was observed, the tendency of blood plasma free radical activity increase, accompanied by the total protein oxidative modification activation, was revealed after the first treatment course. Conclusion. The increase of alpha-1 and gamma globulin levels, as well as an increase of phosphorus level in blood plasma after the first course of polychemotherapy can be used as prognostic factors of anticancer therapy efficiency. High level of endogenous intoxication as well as an increase of copper, iron, zinc and lithium blood plasma concentrations can be applied as the additional markers.

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Local antioxidant effect of original dermal film with melatonin in thermal injury

Bulletin of Russian State Medical University ◽

10.24075/brsmu.2020.070 ◽

2020 ◽

Author(s):

MV Osikov ◽

EV Simonyan ◽

AA Ageeva ◽

YuI Ageev ◽

AA Fedosov ◽

...

Keyword(s):

Thermal Injury ◽

Total Content ◽

Antioxidant Effect ◽

Oxidative Modification ◽

Secondary Products ◽

Burn Wound ◽

Protective Properties ◽

Protein Oxidative Modification ◽

Primary Products ◽

End Products

Oxidative stress (OS) escalation associated with thermal trauma (TT) and pleiotropic effects of melatonin (MT) suggest a study of protective properties of the latter when applied as part of a novel dermal film (DF) to skin burns. This work aimed to assess the content of OS markers in the skin subjected to experimental TT and treated with DF with MT. Third A degree TT (area of 3.5%) were modeled by immersing a patch of skin in boiling water. Twelve cm2 of DF with 5 mg/g of MT were applied daily for 5 days. The parameters calculated were wound's area and epithelializatiohon rate. The products monitored in the burn wound were lipid peroxidation (LPO) products in heptane and isopropanol phases of the lipid extract and protein oxidative modification (POM) products, the modification being spontaneous and metal-dependent. With TT in the wound, the content of secondary and end LPO products in heptane and isopropanol phases increased on the 5th and 10th days; the total content of POM products grew on the 5th day (primary products, neutral) and on the 10th day (primary and secondary products, neutral). Application of DF to a TT wound reduced the burn area, increased the epithelialization rate (by the 10th day, the median went from 1.90% to 6.57%; p < 0.05), reduced the content of secondary and end LPO products in isopropanol phase (by the 10th day, the median went from 0.007 to 0.004 u.o.i; p < 0.05), reduced the total content of OMP products, namely that of primary neutral products — on the 5th day, of primary and secondary neutral products — on the 10th day. With TT present in the context of MT application, the burn area showed presence of secondary LPO products in heptane and isopropanol phases, LPO end products in isopropanol phase, POM products in the wound (basic and neutral primary/secondary POM products).

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