scholarly journals Local antioxidant effect of original dermal film with melatonin in thermal injury

2020 ◽  
Author(s):  
MV Osikov ◽  
EV Simonyan ◽  
AA Ageeva ◽  
YuI Ageev ◽  
AA Fedosov ◽  
...  
Keyword(s):  
Thermal Injury ◽  
Total Content ◽  
Antioxidant Effect ◽  
Oxidative Modification ◽  
Secondary Products ◽  
Burn Wound ◽  
Protective Properties ◽  
Protein Oxidative Modification ◽  
Primary Products ◽  
End Products

Oxidative stress (OS) escalation associated with thermal trauma (TT) and pleiotropic effects of melatonin (MT) suggest a study of protective properties of the latter when applied as part of a novel dermal film (DF) to skin burns. This work aimed to assess the content of OS markers in the skin subjected to experimental TT and treated with DF with MT. Third A degree TT (area of 3.5%) were modeled by immersing a patch of skin in boiling water. Twelve cm2 of DF with 5 mg/g of MT were applied daily for 5 days. The parameters calculated were wound's area and epithelializatiohon rate. The products monitored in the burn wound were lipid peroxidation (LPO) products in heptane and isopropanol phases of the lipid extract and protein oxidative modification (POM) products, the modification being spontaneous and metal-dependent. With TT in the wound, the content of secondary and end LPO products in heptane and isopropanol phases increased on the 5th and 10th days; the total content of POM products grew on the 5th day (primary products, neutral) and on the 10th day (primary and secondary products, neutral). Application of DF to a TT wound reduced the burn area, increased the epithelialization rate (by the 10th day, the median went from 1.90% to 6.57%; p < 0.05), reduced the content of secondary and end LPO products in isopropanol phase (by the 10th day, the median went from 0.007 to 0.004 u.o.i; p < 0.05), reduced the total content of OMP products, namely that of primary neutral products — on the 5th day, of primary and secondary neutral products — on the 10th day. With TT present in the context of MT application, the burn area showed presence of secondary LPO products in heptane and isopropanol phases, LPO end products in isopropanol phase, POM products in the wound (basic and neutral primary/secondary POM products).

647 Development of a Cerium (III) Nitrate-containing Electrospun Dressing for Mitigating Delayed Eschar Removal

2020 ◽  
Vol 41 (Supplement_1) ◽  
pp. S169-S170
Author(s):  
Angela R Jockheck-Clark ◽  
Cortes Williams ◽  
Christine Kowalczewski ◽  
Jahnabi Roy ◽  
Marc A Thompson ◽  
...  
Keyword(s):  
Room Temperature ◽  
Thermal Injury ◽  
Storage Stability ◽  
Wound Dressings ◽  
High Rate ◽  
Burn Wound ◽  
Burn Treatment ◽  
N Application ◽  
Surgical Interventions ◽  
N Release

Abstract Introduction During periods of delayed burn treatment, cells within the eschar leach toxic and immunomodulatory metabolites that can profoundly impact neighboring tissue. Therefore, to reduce the burn-related morbidities and mortalities that are the result of delayed surgical interventions, electrospinning was utilized to generate a novel cerium (III) nitrate (Ce(III)N) dressing. Previously published work has demonstrated that topical Ce(III)N application changes the eschar morphology, and that tissue beneath the treated eschar was generally healthy and had a high rate of graft acceptance. Methods Ce(III)N was dissolved with polyethylene oxide and spun onto a grounded rotating mandrel. The uni-axially spun mesh was compared to a co-axially electrospun dressing that contained a Ce(III)N core. Dressings were evaluated for topography/morphology, porosity and oxygen permeation using scanning electron microscopy, helium pycnometry, and a gas exchange chamber, respectively. Ce(III)N release rates were evaluated, as well as 60-day storage stability. Results All electrospun dressings contained functional Ce(III)N, with the co-axially spun dressing containing three times the amount of Ce(III)N as the traditionally spun dressing. Uni-axially and co-axially spun nanofibers had diameters of 1487±560 nm and 1071±147 nm, and porosities of 83.9% and 74.1%, respectively. Scaffolds released the majority of Ce(III)N within the first hour of wetting. Conclusions All dressings were capable of a burst of Ce(III)N release and maintained stability when stored at room temperature for 60 days. Applicability of Research to Practice Despite advancement in protective equipment worn by military personnel, the incidence of thermal injury is expected to rise in future conflicts. There are no burn wound dressings that can mitigate the pathophysiological processes associated with delayed burn wound treatment.

scholarly journals PECULIARITIES OF THE WOUND PROCESS DEVELOPMENT IN PATIENTS WITH THERMAL INJURY WHEN USED SILVER PREPARATIONS IN BURN WOUNDS SURGICAL TREATMENT

2021 ◽  
pp. 81-87
Author(s):  
N. V. Tuzyuk
Keyword(s):  
Metabolic Activity ◽  
Functional Activity ◽  
Thermal Injury ◽  
Thermal Damage ◽  
Main Group ◽  
The Body ◽  
Metabolic Reaction ◽  
Neutrophilic Granulocytes ◽  
Burn Wound ◽  
Significant Activation

Fundamental research in the field of burn wound development process highlights new pathogenetic mechanisms that underlie the improvement of medical practice, the main purpose of which is to create optimal conditions for early regeneration of the affected skin, as well as to eliminate the development of local and general complications. Irrational general and local treatment of wounds contribute to the slowing down of regenerative processes, thereby worsening the prognosis and consequences of thermal injury. It was found out that in patients of both study groups in the first period of the study the characteristics of the cellular composition of the wound impressions of the wound bottom of the area of thermal damage correspond to the degenerative type. In patients of the main group in the second term of the study in the area of thermal damage cells with high levels of functional and metabolic activity begin to appear, which increase antimicrobial resistance and reduce the risk of generalization of the infectious process which is the main source of burns. In patients of the main group, an increase in the activity of neutrophilic granulocytes in the induced test was found in the second term of the study, which, in our opinion, indicates the involvement of neutrophilic granulocytes with high functional and metabolic activity. Under conditions of significant activation of resident monocytes in response to injury in patients of the main group in the second and third term, the area of thermal damage migrates functionally effective monocytes. In patients of the main group the preservation of the activity of myeloperoxidase - the main enzyme of the oxygen-dependent phase of phagocytosis at the minimum subcompensated level, which leads to the preservation of the oxygen-dependent metabolic reaction of neutrophilic granulocytes in the subsequent study, Increases in the content of PAS-positive substances in neutrophilic granulocytes indicate the preservation of the functional reserve for activation and completion of the oxygen-independent phase of phagocytosis. The use of the developed treatment promotes the involvement of functionally active phagocytic cells in the burn wound, reduces the toxic load on monocytes and neutrophils, which helps to maintain their functional activity at the subcompensated level. These trends in patients of the main group reduce the risk of both local and general infectious complications of burn disease. It was found that the level of spontaneous functional activity in the HCT test is associated with the activity of resident monocytes. They form the primary barrier that protects the body from infection or harmful macromolecular complexes. Monocytes - "inflammatory", which constantly come from the free pool and their functional activity against microbial antigens is much higher than resident monocytes. An indicator of the activity of monocytes - "inflammatory" is an indicator of functional activity in the induced HCT test. The results of the study indicate that under conditions of significant activation of resident monocytes in response to injury in patients of the main group in the second and third terms of the area of thermal damage migrate functionally effective monocytes.

scholarly journals Bacterial Translocation and Intestinal Atrophy After Thermal Injury and Burn Wound Sepsis

1990 ◽  
Vol 211 (4) ◽  
pp. 399-405 ◽  
Author(s):  
WILLIAM G. JONES ◽  
JOSEPH P. MINEL ◽  
ANNABEL E. BARBER ◽  
JOSEPH L. RAYBURN ◽  
THOMAS J. FAHEY ◽  
...  
Keyword(s):  
Bacterial Translocation ◽  
Thermal Injury ◽  
Burn Wound ◽  
Wound Sepsis ◽  
Intestinal Atrophy

Transformations of β-aryl-N-Cbz-α,β-didehydro-α-amino esters with hydrazine hydrate

2016 ◽  
Vol 71 (6) ◽  
pp. 623-631
Author(s):  
Miha Drev ◽  
Uroš Grošelj ◽  
Jurij Svete
Keyword(s):  
Reaction Time ◽  
Hydrazine Hydrate ◽  
Reductive Cleavage ◽  
Secondary Products ◽  
Amino Esters ◽  
Primary Products

AbstractCyclizations of Cbz-protected α,β-didehydro-β-arylalanine esters 1 with excess hydrazine hydrate afforded mixtures of the expected 3-pyrazolidinones 2 and the unexpected 1-amino-5-benzylidenehydantoins 6 and N-Cbz-β-arylalanine hydrazides 7. Presumably, the pyrazolidinones 2 and hydantoins 6 are formed as primary products via competitive 1,2- and 1,4-addition of hydrazine hydrate followed by cyclization, whereas β-arylalanine hydrazides 7 are formed as secondary products via reductive cleavage of the C(5)–N(1) bond in pyrazolidinones 2. The overall selectivity depends on the reaction time and on the β-substituent in the starting dehydroalanine ester 1.

Different effects of thiol and nonthiol ace inhibitors on copper-induced lipid and protein oxidative modification

1996 ◽  
Vol 20 (4) ◽  
pp. 507-514 ◽  
Author(s):  
Afonso C Fernandes ◽  
Paulo M Filipe ◽  
JoÃo P Freitas ◽  
Carlos F Manso
Keyword(s):  
Ace Inhibitors ◽  
Oxidative Modification ◽  
Protein Oxidative Modification

scholarly journals The velocity of corrosion from the electrochemical standpoint

1931 ◽  
Vol 131 (817) ◽  
pp. 355-375 ◽  
Keyword(s):  
Nitrate Solution ◽  
Electrochemical Process ◽  
Corrosion Process ◽  
Ferrous Chloride ◽  
Secondary Products ◽  
Electric Currents ◽  
Primary Products ◽  
Metallic Object ◽  
Set Up ◽  
Direct Attack

It is now agreed that the wet corrosion of metals is usually an electrochemical process, accompanied by the flow of electric currents over measurable distances. Direct combination, without appreciable flow of current, is indeed met with in some cases, for instance, in corrosion by a solution of a halogen. But direct attack by dissolved oxygen would lead to the production of a film of oxide, which, being sparingly soluble, would restrain further action. Indirect electrochemical action by oxygen, in the presence of a salt, will, however, often yield soluble bodies as the primary products; thus iron in sodium chloride solution containing oxygen, fields ferrous chloride at the anodic areas and sodium hydroxide at the cathodic areas. Where these primary products meet, in presence of oxygen, they yields a mixture of hydrated iron oxides known as "rust"; but such secondary products—although almost insoluble— are usually precipitated at a sensible distance from the metal, and do not protect it from further attack. In cases where electrochemical action would lead to a sparingly soluble salt as the direct product, the attack does not develop; thus lead, although rapidly attacked by a nitrate solution in presence of oxygen, is hardly affected by a sulphate solution. The electric currents involved in the corrosion-process may be set up:— ( a ) By differences in the metallic object ( e. g ., on a "bimetallic" specimen, where one metal serves as cathode and the other as anode; or on iron partly covered with mill-scale, which serves as cathode, the exposed metal acting as anode).

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