Isolation and characterization of salt-tolerant glutaminases from marine Micrococcus luteus K-3

1994 ◽  
Vol 77 (6) ◽  
pp. 621-625 ◽  
Author(s):  
Mitsuaki Moriguchi ◽  
Kenji Sakai ◽  
Ryoji Tateyama ◽  
Yoichi Furuta ◽  
Mamoru Wakayama
Keyword(s):  
Micrococcus Luteus ◽  
Salt Tolerant ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Antibacterial Carotane Sesquiterpenes from Artemisia argyi Associated Endophytic Trichoderma virens QA-8

Antibiotics ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 213
Author(s):  
Xiao-Shan Shi ◽  
Yin-Ping Song ◽  
Ling-Hong Meng ◽  
Sui-Qun Yang ◽  
Dun-Jia Wang ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Micrococcus Luteus ◽  
Crystallographic Analysis ◽  
Medicinal Herb ◽  
Trichoderma Virens ◽  
Isolation And Characterization ◽  
Artemisia Argyi ◽  
Detailed Interpretation ◽  
Fungal Kingdom

Carotane sesquiterpenes are commonly found in plants but are infrequently reported in the fungal kingdom. Chemical investigation of Trichoderma virens QA-8, an endophytic fungus associated with the inner root tissue of the grown medicinal herb Artemisia argyi H. Lév. and Vaniot, resulted in the isolation and characterization of five new carotane sesquiterpenes trichocarotins I–M (1–5), which have diverse substitution patterns, and seven known related analogues (6–12). The structures of these compounds were established on the basis of a detailed interpretation of their NMR and mass spectroscopic data, and the structures including the relative and absolute configurations of compounds 1–3, 5, 9, and 10 were confirmed by X-ray crystallographic analysis. In the antibacterial assays, all isolates exhibited potent activity against Escherichia coli EMBLC-1, with MIC values ranging from 0.5 to 32 µg/mL, while 7β-hydroxy CAF-603 (7) strongly inhibited Micrococcus luteus QDIO-3 (MIC = 0.5 µg/mL). Structure-activity relationships of these compounds were discussed. The results from this study demonstrate that the endophytic fungus T. virens QA-8 from the planted medicinal herb A. argyi is a rich source of antibacterial carotane sesquiterpenes, and some of them might be interesting for further study to be developed as novel antibacterial agents.

scholarly journals A salt tolerant Sphingosinicella microcystinivorans A3 isolated from soil contaminated with mercury in traditional gold mining of Jendi Village, Wonogiri District, Indonesia

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
Sutami SUTAMI ◽  
Purwanto PURWANTO ◽  
Retno Rosariastuti
Keyword(s):  
Gold Mining ◽  
Rrna Gene ◽  
Salt Tolerant ◽  
Indigenous Bacteria ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Query Coverage ◽  
Physiological Tests ◽  
Indole Production

Abstract. Sutami, Purwanto, Rosariastuti R. 2021. A salt tolerant Sphingosinicella microcystinivorans A3 isolated from soil contaminated with mercury in traditional gold mining of Jendi Village, Wonogiri District, Indonesia. Biodiversitas 22: 3785-3791. Isolation and characterization of indigenous bacteria from the soil of traditional gold mining contaminated with mercury is the first step in a series of research to explore and utilize indigenous bacteria in Jendi's area. This study was aimed to determine the characteristics and identity of bacterial isolates from soil of traditional gold mining in Jendi Village, Wonogiri contaminated by mercury. The methods used in this study included bacterial isolation, media preparation, phenotypic identification including; morphological and physiological tests and genotyping tests. The results showed that the bacterial isolate A3 grew optimally in media with the addition of 10% NaCl, at a temperature of 27°C, and pH 9. There were negative reactions to the observations of gram staining, acid production from glucose, indole production, catalase and urease, and positive reactions to oxidation. A neighbor-joining phylogenetic tree based on the 16S rRNA gene sequence showed that the A3 strain was closely related to Sphingosinicella microcystinivorans strain Y2T (JCM 13185T) with 100% Query coverage and a maximum identity of 99.56%.

scholarly journals Isolation and characterization of aerial bacteria from a microbiology laboratory environment

2019 ◽  
Vol 26 ◽  
pp. 41-48
Author(s):  
Usman AN ◽  
J Aliyu ◽  
B Christiana ◽  
U Umar ◽  
AI Adedayo ◽  
...  
Keyword(s):  
Micrococcus Luteus ◽  
Bacterial Load ◽  
Frequency Of Occurrence ◽  
Bacterial Isolates ◽  
Hand Sanitizer ◽  
Isolation And Characterization ◽  
Diffusion Technique ◽  
Cleaning And Disinfection ◽  
Outer Environment

Sterile nutrient agar (NA) plates were exposed to the inner and outer environment of the laboratory for 15 minutes before the commencement of laboratory work in the morning and after closure of activities in the evening after which the plates were incubated at 37oC for 24 hours. Antibiotic susceptibility of the bacterial isolates was done using agar well diffusion technique. The aerial bacterial load outside the laboratory was found to be higher than inside the laboratory. Bacteria isolated include members of the genus Staphylococcus, Bacillus, Lactobacillus, Salmonella, Micrococcus, Streptococcus, Proteus and Escherichia. Bacillus subtilis had the highest frequency of occurrence (31.0%) followed by alpha haemolytic streptococcus (19.0%) while the least frequency of occurrence was shown by Proteus bulgaricus and Proteus species (1.7%). All the bacteria were sensitive to antibiotics tested except Micrococcus luteus. They were equally found to be sensitive to the anti-microbial agent (antiseptic and disinfectant) tested except for the hand sanitizer to which most isolates displayed high resistance. The presence of a wide variety of pathogenic microorganisms in the laboratory shows that the environment requires regular cleaning and disinfection to enhance the safety of staff and students using the laboratory. J. bio-sci. 26: 41-48, 2018

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