scholarly journals Isolation and Characterization of Antibacterial Carotane Sesquiterpenes from Artemisia argyi Associated Endophytic Trichoderma virens QA-8

Antibiotics ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 213
Author(s):  
Xiao-Shan Shi ◽  
Yin-Ping Song ◽  
Ling-Hong Meng ◽  
Sui-Qun Yang ◽  
Dun-Jia Wang ◽  
...  

Carotane sesquiterpenes are commonly found in plants but are infrequently reported in the fungal kingdom. Chemical investigation of Trichoderma virens QA-8, an endophytic fungus associated with the inner root tissue of the grown medicinal herb Artemisia argyi H. Lév. and Vaniot, resulted in the isolation and characterization of five new carotane sesquiterpenes trichocarotins I–M (1–5), which have diverse substitution patterns, and seven known related analogues (6–12). The structures of these compounds were established on the basis of a detailed interpretation of their NMR and mass spectroscopic data, and the structures including the relative and absolute configurations of compounds 1–3, 5, 9, and 10 were confirmed by X-ray crystallographic analysis. In the antibacterial assays, all isolates exhibited potent activity against Escherichia coli EMBLC-1, with MIC values ranging from 0.5 to 32 µg/mL, while 7β-hydroxy CAF-603 (7) strongly inhibited Micrococcus luteus QDIO-3 (MIC = 0.5 µg/mL). Structure-activity relationships of these compounds were discussed. The results from this study demonstrate that the endophytic fungus T. virens QA-8 from the planted medicinal herb A. argyi is a rich source of antibacterial carotane sesquiterpenes, and some of them might be interesting for further study to be developed as novel antibacterial agents.

2009 ◽  
Vol 8 (2) ◽  
pp. 194-203 ◽  
Author(s):  
S.S.L. Oh ◽  
F.D.A. Bakar ◽  
A.M. Adnan ◽  
N.M. Mahadi ◽  
O. Hassan ◽  
...  

2019 ◽  
Vol 26 ◽  
pp. 41-48
Author(s):  
Usman AN ◽  
J Aliyu ◽  
B Christiana ◽  
U Umar ◽  
AI Adedayo ◽  
...  

Sterile nutrient agar (NA) plates were exposed to the inner and outer environment of the laboratory for 15 minutes before the commencement of laboratory work in the morning and after closure of activities in the evening after which the plates were incubated at 37oC for 24 hours. Antibiotic susceptibility of the bacterial isolates was done using agar well diffusion technique. The aerial bacterial load outside the laboratory was found to be higher than inside the laboratory. Bacteria isolated include members of the genus Staphylococcus, Bacillus, Lactobacillus, Salmonella, Micrococcus, Streptococcus, Proteus and Escherichia. Bacillus subtilis had the highest frequency of occurrence (31.0%) followed by alpha haemolytic streptococcus (19.0%) while the least frequency of occurrence was shown by Proteus bulgaricus and Proteus species (1.7%). All the bacteria were sensitive to antibiotics tested except Micrococcus luteus. They were equally found to be sensitive to the anti-microbial agent (antiseptic and disinfectant) tested except for the hand sanitizer to which most isolates displayed high resistance. The presence of a wide variety of pathogenic microorganisms in the laboratory shows that the environment requires regular cleaning and disinfection to enhance the safety of staff and students using the laboratory. J. bio-sci. 26: 41-48, 2018


2008 ◽  
Vol 12 (10) ◽  
pp. 1111-1117 ◽  
Author(s):  
Martin Bröring ◽  
Silke Köhler

The chloroiron complex of 10-acetyl-2,3,7,8,12,13,17,18-octaethylcorrole was isolated as an unexpected product from an attempted preparation of chloroiron-2,2'-bidipyrrin when acetone was used in the solvent mixture. In the molecular structure derived from X-ray crystallographic analysis the orthogonal arrangement of the acetyl group with the corrole macrocycle is clearly apparent, and the UV-vis spectrum of the compound indicates only a small electronic influence of the acetyl group on the corrole π-system. The paramagnetic 1 H NMR spectrum taken at ambient temperature shows two signal sets in a ratio of 2.2:1, indicating two stable orientations of the acetyl group in solution. Attempts to prepare the species from chloroiron-2,2'-bidipyrrin with iron(III)chloride in acetone were successful but gave inconsistent and generally low yields. A rationale for the formation of the new corrole is proposed.


2001 ◽  
Vol 183 (12) ◽  
pp. 3729-3736 ◽  
Author(s):  
Karl-Heinz Gartemann ◽  
Rudolf Eichenlaub

ABSTRACT A new insertion element of 1,449 bp with 25-bp perfect terminal repeats, designated IS1409, was identified in the chromosome of 4-chlorobenzoate-degrading Arthrobactersp. strain TM1 NCIB12013. Upon insertion, IS1409 causes a target duplication of 8 bp. IS1409 carries only a single open reading frame of 435 codons encoding the transposase TnpA. Both TnpA and the overall organization of IS1409are highly similar to those of some related insertion elements of the ISL3 group (J. Mahillon and M. Chandler, Microbiol. Mol. Biol. Rev. 62:725–774, 1998). IS1409 was also found in other 4-chlorobenzoate-degrading Arthrobacter strains and Micrococcus luteus. Based on IS1409, a series of transposons carrying resistance genes for chloramphenicol and gentamicin were constructed. These transposons were used to demonstrate transposition events in vivo and to mutagenizeArthrobacter sp. strains.


2020 ◽  
Vol 8 (12) ◽  
pp. 372-380
Author(s):  
Gunti Vijay Kumar ◽  
◽  
Pandu Brahmaji Rao ◽  

Phenanthrene is the commonly used hazardous chemical in various industries and the detoxification is a big problem till today. In this study, phenanthrene degrading bacteria were isolated from dairy waste samples, characterized by molecular techniques. A total of 10 samples were collected respectively from different spots of dairy industry. Minimum salt agar medium with phenanthrene composition was used to isolate the pure culture of resistant bacterium. Morphological, biochemical tests were done to identify the phenanthrene degrading bacteria. The colonies of the isolates were circular to irregular in dairy samples. Phenanthrene tolerant bacteria were isolated after the dairy waste soil samples was serially diluted and transferred onto M9 minimal agar medium amended with 10mg/l of phenanthrene. A concentration of 10 mg/l was chosen based on the prevailing concentration. Four Phenanthrene tolerant bacteria from dairy waste (HW2, HW1, HW3, HW5) were isolated. From the biochemical and 16srRNA PCR amplification, the bacterium identified was Micrococcus leuteus. The resistance to Phenanthrene by the isolate was tested with various concentrations of Phenanthrene from 0 to 10mM. The Micrococcus luteus showed a MTC value of 28Cfu/ml at 8mM and Bacillus cereus showed least.


2017 ◽  
Vol 67 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Mondem Vasundhara ◽  
Manoj Baranwal ◽  
Nallapeta Sivaramaiah ◽  
Anil Kumar

1994 ◽  
Vol 77 (6) ◽  
pp. 621-625 ◽  
Author(s):  
Mitsuaki Moriguchi ◽  
Kenji Sakai ◽  
Ryoji Tateyama ◽  
Yoichi Furuta ◽  
Mamoru Wakayama

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