Phenotypic characterization of human pertioneal and alveolar macrophages, and of human blood monocytes differentiated in the presence of GM-CSF or M-CSF

Cytokine ◽  
1991 ◽  
Vol 3 (5) ◽  
pp. 460
Keyword(s):  
Human Blood ◽  
Alveolar Macrophages ◽  
Phenotypic Characterization ◽  
Blood Monocytes ◽  
Gm Csf

scholarly journals Characterization of an oligopeptide chemoattractant receptor on human blood monocytes using a new radioligand

Blood ◽  
1984 ◽  
Vol 63 (3) ◽  
pp. 588-592 ◽  
Author(s):  
MC Benyunes ◽  
R Snyderman
Keyword(s):  
Human Blood ◽  
Polymorphonuclear Leukocytes ◽  
Specific Radioactivity ◽  
Blood Monocytes ◽  
Chemotactic Peptide ◽  
Peripheral Blood Monocytes ◽  
Peptide Receptor ◽  
Human Polymorphonuclear Leukocytes ◽  
Dissociation Constant Kd

Abstract The study of chemoattractant receptors on human monocytes had been limited by the lack of a radioligand suitable for use with the small numbers of cells routinely available from human donors. A new synthetic oligopeptide radioligand f[35S]met-leu-phe, with a higher specific radioactivity than was available with the tritiated compound, was used to characterize a chemoattractant receptor on freshly isolated human blood monocytes. These cells bind f[35S]met-leu-phe with a dissociation constant (KD) of 30.2 +/- 5.6 nM and contain 84,000 +/- 11,300 receptors per cell. f[35S]met-leu-phe does not bind specifically to blood lymphocytes. The specificity of the oligopeptide receptor on monocytes is indistinguishable from the oligopeptide chemoattractant receptor on human polymorphonuclear leukocytes. Using f[35S]met-leu- phe, it will now be feasible to study the chemotactic peptide receptor on small numbers of partially purified peripheral blood monocytes from patients with defects of immune function.

scholarly journals In-VitroDifferentiation of Mature Dendritic Cells From Human Blood Monocytes

1998 ◽  
Vol 6 (1-2) ◽  
pp. 25-39 ◽  
Author(s):  
Robert Gieseler ◽  
Dirk Heise ◽  
Afsaneh Soruri ◽  
Peter Schwartz ◽  
J. Hinrich Peters
Keyword(s):  
Dendritic Cells ◽  
Human Blood ◽  
T Cell Response ◽  
Blood Monocytes ◽  
Gm Csf ◽  
Hla Dr ◽  
Hla Dq ◽  
Specific Stimulation ◽  
Antigen Presenting

Representing the most potent antigen-presenting cells, dendritic cells (DC) can now be generated from human blood monocytes. We recently presented a novel protocol employing GM-CSF, IL-4, and IFN-γto differentiate monocyte-derived DCin vitro. Here, such cells are characterized in detail. Cells in culture exhibited both dendritic and veiled morphologies, the former being adherent and the latter suspended. Phenotypically, they were CD1a-/dim, CD11a+, CD11b++, CD11c+, CD14dim/-, CD16a-/dim, CD18+, CD32dim/-, CD33+, CD40+, CD45R0+, CD50+, CD54+, CD64-/dim, CD68+, CD71+, CD80dim, CD86+/++, MHC class I++/+++HLA-DR++/+++HLA-DP+, and HLA-DQ+. The DC stimulated a strong allogeneic T-cell response, and further evidence for their autologous antigen-specific stimulation is discussed. Although resembling a mature CD 11c+CD45R0+blood DC subset identified earlier, their differentiation in the presence of the Thl and Th2 cytokines IFN-γand IL-4 indicates that these DC may conform to mature mucosal DC.

Dyscoordinate Expression of Tumor Necrosis Factor-alpha by Human Blood Monocytes and Alveolar Macrophages

1989 ◽  
Vol 139 (4) ◽  
pp. 1010-1016 ◽  
Author(s):  
Elizabeth A. Rich ◽  
James R. Panuska ◽  
Robert S. Wallis ◽  
Christopher B. Wolf ◽  
Michelle L. Leonard ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Human Blood ◽  
Alveolar Macrophages ◽  
Tumor Necrosis ◽  
Blood Monocytes ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Necrosis Factor

Comparative phenotypic characterization of human cord blood monocytes and placental macrophages at term

Placenta ◽  
2013 ◽  
Vol 34 (9) ◽  
pp. 836-839 ◽  
Author(s):  
S.A. Selkov ◽  
A.V. Selutin ◽  
O.M. Pavlova ◽  
N.N. Khromov-Borisov ◽  
O.V. Pavlov
Keyword(s):  
Cord Blood ◽  
Phenotypic Characterization ◽  
Blood Monocytes ◽  
Human Cord Blood ◽  
Placental Macrophages

MS-based phenotypic characterization of a human blood protein from urinary waste products

2008 ◽  
Vol 2 (7-8) ◽  
pp. 1019-1024 ◽  
Author(s):  
Urban A. Kiernan ◽  
Lisa Hernandez ◽  
Eric E. Niederkofler ◽  
Kemmons A. Tubbs ◽  
Randall W. Nelson
Keyword(s):  
Human Blood ◽  
Phenotypic Characterization ◽  
Blood Protein ◽  
Waste Products

Gene Expression of Macrophage Inflammatory Protein-1αfrom Human Blood Monocytes and Alveolar Macrophages Is Inhibited by Interleukin-4

1993 ◽  
Vol 9 (2) ◽  
pp. 192-198 ◽  
Author(s):  
Theodore J. Standiford ◽  
Steven L. Kunkel ◽  
Janice M. Liebler ◽  
Marie D. Bur dick ◽  
Andrew R. Gilbert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Blood ◽  
Alveolar Macrophages ◽  
Interleukin 4 ◽  
Blood Monocytes ◽  
Inflammatory Protein ◽  
Macrophage Inflammatory Protein

An In Vitro Method for Examining the Effects of Human Monocytes on the Durability of Glass Fibres

1988 ◽  
Vol 16 (2) ◽  
pp. 155-161
Author(s):  
Paul J. Conroy ◽  
John Young ◽  
David G. Dewhurst ◽  
David W. Allen ◽  
Anthony Clark
Keyword(s):  
Human Blood ◽  
Glass Fibre ◽  
Alveolar Macrophages ◽  
Human Monocytes ◽  
Blood Monocytes ◽  
Isolated Cells ◽  
In Vitro Method ◽  
Glass Fibres ◽  
Dynamic Cell

An in vitro method for characterising the degradation of glass fibres in an intramacrophage-type environment is reported. Human blood monocytes provide the phagocytically active cultures and a “semi-dynamic cell replenishment method”, whereby cultures are replenished every three weeks with monocytes from the same donor, has enabled exposure times exceeding 26 weeks to be achieved. Tests indicated an affinity of monocytes for glass fibre, and superoxide activity in freshly isolated cells was measured and found to be comparable with that for alveolar macrophages.

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