Diagnosis of Trichomonas vaginalis in adolescent females: ‘Inpouch TV’ culture versus wet mount microscopy

1996 ◽  
Vol 18 (2) ◽  
pp. 117
Author(s):  
Chris L. Ohlemeyer ◽  
Laurie L. Hornberger ◽  
Daryl A. Lynch ◽  
Ella M. Swierkosz
Keyword(s):  
Trichomonas Vaginalis ◽  
Adolescent Females ◽  
Wet Mount ◽  
Tv Culture

Diagnosis of Trichomonas vaginalis in adolescent females: InPouch TV® culture versus wet-mount microscopy

1998 ◽  
Vol 22 (3) ◽  
pp. 205-208 ◽  
Author(s):  
Chris L. Ohlemeyer ◽  
Laurie L. Hornberger ◽  
Daryl A. Lynch ◽  
Ella M. Swierkosz
Keyword(s):  
Trichomonas Vaginalis ◽  
Adolescent Females ◽  
Wet Mount

scholarly journals Comparison of Trichomoniasis Diagnosis by Microscopic Methods and Indirect ELISA Technique in a Sample of Iraqi Women

2020 ◽  
pp. 742-748
Author(s):  
Nada H. Bedair ◽  
Hayder Z. Ali
Keyword(s):  
Trichomonas Vaginalis ◽  
Vaginal Discharge ◽  
Indirect Elisa ◽  
Elisa Method ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Wet Mount ◽  
Urine Examination ◽  
Iraqi Women ◽  
Elisa Technique

Trichomonas vaginalis is an eukaryotic parasite that causes the most common non-viral sexually transmitted infection, trichomoniasis. This disease is responsible for many serious health problems such as preterm birth. More than half of the infected women do not develop symptoms, which makes it difficult to diagnose thedisease. In this study, a specific indirect ELISA method was developed to detect anti-Trichomonas vaginalis IgM and IgG immunoglobulins in the sera of infected females. The aim of this study was to investigate the sensitivity of a simple ELISA procedure in comparison to the classical urine examination and vaginal wet mount preparation for the diagnosis of T. vaginalis. The sensitivity of the indirect ELISA was compared with the classical vaginal discharge swab and urine microscopic examination, and the results showed sensitivities of 65.5% and 57.2%, respectively. Furthermore, the infection was measurable as acute or chronic with the refined test.

scholarly journals Real-time PCRs for detection of Trichomonas vaginalis β-tubulin and 18S rRNA genes in female genital specimens

2007 ◽  
Vol 56 (6) ◽  
pp. 772-777 ◽  
Author(s):  
Paul Simpson ◽  
Geoff Higgins ◽  
Ming Qiao ◽  
Russell Waddell ◽  
Tuckweng Kok
Keyword(s):  
Real Time ◽  
Trichomonas Vaginalis ◽  
18S Rrna ◽  
Clinical Manifestations ◽  
Developed Countries ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Wet Mount ◽  
Increased Risk ◽  
18S Rrna Genes

Trichomonas vaginalis is the cause of one of the most common types of vaginitis, trichomoniasis. The incidence of trichomoniasis in developed countries has decreased substantially during the past decade, but high prevalence of this disease can still be found in rural and remote areas of Australia. Clinical manifestations of symptomatic women are generally non-specific, but include vaginal discharge, vaginitis and irritation. T. vaginalis infection has also been linked to the increased risk of human immunodeficiency virus transmission. Current diagnosis of T. vaginalis relies on the visualization of motile organisms in a wet-mount preparation. Culture is used mainly in reference laboratories. The latter two methods require viable organisms and would not be suitable for use where transportation of specimens can be delayed. Two real-time fluorescence resonance energy transfer (FRET) hybridization probe PCR assays were used in this study to test for T. vaginalis DNA, targeting the β-tubulin and 18S rRNA genes. We tested 500 randomly selected female patients, in an STD setting, for T. vaginalis DNA. The FRET PCRs targeting the β-tubulin gene and the 18S rRNA gene detected 96 % (85/89) and 100 % (89/89) , respectively, of the positive specimens (first-void urine sample or genital swabs). Wet-mount microscopy was performed on 76 of these PCR-positive specimens and showed a sensitivity of 38 % (29/76). The prevalence, by PCR, of trichomoniasis was 18 % in this study. The two real-time PCRs developed in this study, targeting different genetic regions of the organism, provide a rapid, sensitive and specific diagnosis of T. vaginalis infection.

The prevalence of Trichomonas vaginalis detected by wet mount and polymerase chain reaction in Sydney women

Sexual Health ◽  
2013 ◽  
Vol 10 (4) ◽  
pp. 385 ◽  
Author(s):  
Ivy Kwon ◽  
Anna McNulty ◽  
Phillip Read
Keyword(s):  
Polymerase Chain Reaction ◽  
Trichomonas Vaginalis ◽  
Health Centre ◽  
Chain Reaction ◽  
Local Data ◽  
Cross Sectional ◽  
Wet Mount ◽  
Polymerase Chain ◽  
Sexually Transmissible Infection ◽  
Transmissible Infection

Objectives Although Trichomonas vaginalis (TV) has a low profile in urban Australia, local data has estimated the prevalence in women to be 10 times higher when using polymerase chain reaction (PCR) versus wet mount microscopy (4.8% v. 0.4%). Our aim was to determine the prevalence of TV in Sydney women using both wet mount and PCR. Methods: A cross-sectional study was conducted of women requiring sexually transmissible infection screening at the Sydney Sexual Health Centre. Vaginal swabs were examined for TV using PCR and wet mount microscopy. Results: In total, 781 of 1263 eligible women were tested; 3 out of 781 tested positive by PCR and 1 out of 781 by wet mount, giving a prevalence of 0.38% (95% confidence interval (CI): 0.14–1.12%) and 0.13% (95% CI: 0.03–0.71%) respectively. There was not enough power to compare PCR and wet mount. Conclusions: The results of this analysis indicate that in our female urban population, TV is a very rare sexually transmissible infection,with 0.38% prevalence, and routine screening by PCR is not indicated.

scholarly journals Diagnostic Performance of a Molecular Test versus Clinician Assessment of Vaginitis

2018 ◽  
Vol 56 (6) ◽  
Author(s):  
Jane R. Schwebke ◽  
Charlotte A. Gaydos ◽  
Paul Nyirjesy ◽  
Sonia Paradis ◽  
Salma Kodsi ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Trichomonas Vaginalis ◽  
The United States ◽  
Cross Sectional Study ◽  
Molecular Test ◽  
Cross Sectional ◽  
Content Type ◽  
Wet Mount ◽  
Reference Methods ◽  
Higher Sensitivity

ABSTRACTVaginitis is a common complaint, diagnosed either empirically or using Amsel's criteria and wet mount microscopy. This study sought to determine characteristics of an investigational test (a molecular test for vaginitis), compared to reference, for detection of bacterial vaginosis,Candidaspp., andTrichomonas vaginalis. Vaginal specimens from a cross-sectional study were obtained from 1,740 women (≥18 years old), with vaginitis symptoms, during routine clinic visits (across 10 sites in the United States). Specimens were analyzed using a commercial PCR/fluorogenic probe-based investigational test that detects bacterial vaginosis,Candidaspp., andTrichomonas vaginalis. Clinician diagnosis and in-clinic testing (Amsel's test, potassium hydroxide preparation, and wet mount) were also employed to detect the three vaginitis causes. All testing methods were compared to the respective reference methods (Nugent Gram stain for bacterial vaginosis, detection of theCandidageneits2, andTrichomonas vaginalisculture). The investigational test, clinician diagnosis, and in-clinic testing were compared to reference methods for bacterial vaginosis,Candidaspp., andTrichomonas vaginalis. The investigational test resulted in significantly higher sensitivity and negative predictive value than clinician diagnosis or in-clinic testing. In addition, the investigational test showed a statistically higher overall percent agreement with each of the three reference methods than did clinician diagnosis or in-clinic testing. The investigational test showed significantly higher sensitivity for detecting vaginitis, involving more than one cause, than did clinician diagnosis. Taken together, these results suggest that a molecular investigational test can facilitate accurate detection of vaginitis.

Performance of the OSOM Trichomonas Rapid Test for diagnosis of Trichomonas vaginalis infection among women in Mysore, India

Sexual Health ◽  
2013 ◽  
Vol 10 (4) ◽  
pp. 320 ◽  
Author(s):  
Purnima Madhivanan ◽  
Tan Li ◽  
Stephanie Trammell ◽  
Chirayu Desai ◽  
Vijaya Srinivas ◽  
...  
Keyword(s):  
False Positive ◽  
Trichomonas Vaginalis ◽  
Rapid Test ◽  
Health Clinic ◽  
Resource Constrained ◽  
Short Supply ◽  
Predictive Values ◽  
Wet Mount ◽  
Kappa Agreement ◽  
Culture Positive

Background Trichomonas vaginalis is the world’s most common treatable sexually transmissible infection. Currently, wet mount microscopy and syndromic management based on vaginal discharge are the most widely used methods for diagnosing and treating trichomoniasis in resource-constrained settings. Wet mount microscopy requires equipment and trained technicians, who are in short supply. We examined the diagnostic accuracy of the OSOM Trichomonas Rapid Test for detecting T. vaginalis vaginal infection among women in Mysore, India. Methods: During July 2009–August 2010, 450 sexually active women over 18 years seeking care at an urban reproductive health clinic were enrolled in the study. Clinician-collected vaginal swabs were evaluated for trichomonads using wet mount microscopy, InPouch culture and the OSOM test. Results: Of the 418 samples included in the analyses, culture detected 68 (16.3%) positive samples, wet mount microscopy detected 56 of the culture-positive samples and four false positive samples. The OSOM test detected 60 of the culture-positive samples plus two false positive cases. Using the composite reference standard (CRS), defined as wet mount- or culture-positive, the sensitivities of wet mount, the OSOM test and culture were 83.3%, 86.1% and 94.4%, respectively. The positive and negative predictive values of the OSOM test were 100% and 97.1% respectively. The Cohen’s kappa agreement between the OSOM test and the CRS was excellent (κ = 0.94). Conclusion: The OSOM test has high sensitivity, excellent specificity, and excellent positive and negative predictive value compared to a CRS. This simple test can improve screening and diagnosis of T. vaginalis infection in resource-constrained settings where microscopy and culture are unavailable.

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