ESTIMATION OF DEXTROSE EQUIVALENT VALUE OF STARCH HYDROLYSATES FROM LIQUID CHROMATOGRAPHIC PROFILES

Author(s):  
Donald L. Kiser ◽  
Robert L. Hagy
1980 ◽  
Vol 26 (6) ◽  
pp. 707-711 ◽  
Author(s):  
T D Schlabach ◽  
J A Fulton ◽  
P B Mockridge ◽  
E C Toren

Abstract We observed nonenzymic peaks when serum isoenzymes of lactate dehydrogenase (EC 1.1.1.27; LD) and creatine kinase (EC 2.7.3.2.; CK) were separated by “high-performance” liquid chromatography and detected by continuously monitoring the column effluent for enzyme activity. Such background peaks were particularly apparent in CK isoenzyme profiles obtained from human sera. We observed two nonenzymic peaks with fluorescence detection, one in the CK-MB region, the other in the CK-BB region. Serum albumin was a major component in the artifactual CK-MB peak, with lipoprotein as a minor component. We present evidence that the material responsible for the other peak fluoresced quite strongly and is mostly pre-albumin.


2000 ◽  
Vol 886 (1-2) ◽  
pp. 123-131 ◽  
Author(s):  
W. Vetter ◽  
N.Piñeiro Costas ◽  
R. Bartha ◽  
A. Gago Martı́nez ◽  
B. Luckas

1988 ◽  
Vol 71 (2) ◽  
pp. 397-403 ◽  
Author(s):  
Samy H Ashoor ◽  
Woodrow C Monte ◽  
Philip G Stiles

Abstract Raw beef, pork, veal, lamb, chicken, turkey, and duck have been identified with a liquid chromatographic (LC) method. Meat samples are blended in water, and soluble proteins in the aqueous blends are separated by the LC method. Meat cuts and parts from same species had similar chromatographic profiles and differed only quantitatively. However, meat cuts or parts from different species resulted in different chromatographic profiles. The qualitative and quantitative chromatographic differences among meat species were used for their identification. The LC method applies only to fresh and frozen meats. It is simple, rapid, and reliable, and can be used for quantitative detection of meat species in unheated meat blends.


1980 ◽  
Vol 26 (6) ◽  
pp. 707-711
Author(s):  
T D Schlabach ◽  
J A Fulton ◽  
P B Mockridge ◽  
E C Toren

Abstract We observed nonenzymic peaks when serum isoenzymes of lactate dehydrogenase (EC 1.1.1.27; LD) and creatine kinase (EC 2.7.3.2.; CK) were separated by “high-performance” liquid chromatography and detected by continuously monitoring the column effluent for enzyme activity. Such background peaks were particularly apparent in CK isoenzyme profiles obtained from human sera. We observed two nonenzymic peaks with fluorescence detection, one in the CK-MB region, the other in the CK-BB region. Serum albumin was a major component in the artifactual CK-MB peak, with lipoprotein as a minor component. We present evidence that the material responsible for the other peak fluoresced quite strongly and is mostly pre-albumin.


Sign in / Sign up

Export Citation Format

Share Document