Interferences appearing in fluorometrically measured liquid-chromatographic profiles of creatine kinase isoenzymes in serum.

1980 ◽  
Vol 26 (6) ◽  
pp. 707-711 ◽  
Author(s):  
T D Schlabach ◽  
J A Fulton ◽  
P B Mockridge ◽  
E C Toren
Keyword(s):  
Creatine Kinase ◽  
High Performance ◽  
Minor Component ◽  
The Other ◽  
Present Evidence ◽  
Human Sera ◽  
A Minor ◽  
Liquid Chromatographic ◽  
Measured Liquid ◽  
Chromatographic Profiles

Abstract We observed nonenzymic peaks when serum isoenzymes of lactate dehydrogenase (EC 1.1.1.27; LD) and creatine kinase (EC 2.7.3.2.; CK) were separated by “high-performance” liquid chromatography and detected by continuously monitoring the column effluent for enzyme activity. Such background peaks were particularly apparent in CK isoenzyme profiles obtained from human sera. We observed two nonenzymic peaks with fluorescence detection, one in the CK-MB region, the other in the CK-BB region. Serum albumin was a major component in the artifactual CK-MB peak, with lipoprotein as a minor component. We present evidence that the material responsible for the other peak fluoresced quite strongly and is mostly pre-albumin.

Interferences appearing in fluorometrically measured liquid-chromatographic profiles of creatine kinase isoenzymes in serum.

1980 ◽  
Vol 26 (6) ◽  
pp. 707-711
Author(s):  
T D Schlabach ◽  
J A Fulton ◽  
P B Mockridge ◽  
E C Toren
Keyword(s):  
Creatine Kinase ◽  
High Performance ◽  
Minor Component ◽  
The Other ◽  
Present Evidence ◽  
Human Sera ◽  
A Minor ◽  
Liquid Chromatographic ◽  
Measured Liquid ◽  
Chromatographic Profiles

Abstract We observed nonenzymic peaks when serum isoenzymes of lactate dehydrogenase (EC 1.1.1.27; LD) and creatine kinase (EC 2.7.3.2.; CK) were separated by “high-performance” liquid chromatography and detected by continuously monitoring the column effluent for enzyme activity. Such background peaks were particularly apparent in CK isoenzyme profiles obtained from human sera. We observed two nonenzymic peaks with fluorescence detection, one in the CK-MB region, the other in the CK-BB region. Serum albumin was a major component in the artifactual CK-MB peak, with lipoprotein as a minor component. We present evidence that the material responsible for the other peak fluoresced quite strongly and is mostly pre-albumin.

scholarly journals Analytical Determination of Virginiamycin Drug Residues in Edible Porcine Tissues by LC-MS with Confirmation by LC-MS/MS

2009 ◽  
Vol 92 (1) ◽  
pp. 329-339 ◽  
Author(s):  
Joe Boison ◽  
Stephen Lee ◽  
Ron Gedir
Keyword(s):  
Solid Phase ◽  
Minor Component ◽  
Analytical Determination ◽  
Porcine Liver ◽  
Solid Phase Extraction Cartridge ◽  
Muscle Tissues ◽  
Dried Extract ◽  
A Minor ◽  
Liquid Chromatographic

Abstract A liquid chromatographic-mass spectrometric (LC-MS) method was developed and validated for the determination and confirmation of virginiamycin (VMY) M1 residues in porcine liver, kidney, and muscle tissues at concentrations 2 ng/g. Porcine liver, kidney, or muscle tissue is homogenized with methanolacetonitrile. After centrifugation, the supernatant is diluted with phosphate buffer and cleaned up on a C18 solid-phase extraction cartridge. VMY in the eluate is partitioned into chloroform and the aqueous upper layer is removed by aspiration. After evaporating the chloroform in the residual mixture to dryness, the dried extract is reconstituted in mobile phase and VMY is quantified by LC-MS. Any samples eliciting quantifiable levels of VMY M1 (i.e., at concentrations 2 ng/g) are subjected to confirmatory analysis by LC-MS/MS. VMY S1, a minor component of the VMY complex, is monitored but not quantified or confirmed.

Liquid-chromatographic measurement of p-aminobenzoic acid and its metabolites in serum.

1988 ◽  
Vol 34 (11) ◽  
pp. 2235-2238 ◽  
Author(s):  
L L Yung-Jato ◽  
P R Durie ◽  
S J Soldin
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Peak Height ◽  
Individual Compound ◽  
Aminobenzoic Acid ◽  
Serum Samples ◽  
The Individual ◽  
A Minor ◽  
Liquid Chromatographic

Abstract This is a high-performance liquid-chromatographic method for measuring p-aminobenzoic acid (PABA) and its metabolites in plasma or serum. Samples are deproteinized, then extracted with organic solvents before chromatography. For quantification, the peak height of the individual compound is compared with that of the internal standard. Analytical recoveries ranged from 41% to 100%, depending on the compound studied. Comparison of patients' samples after oral administration of either N-benzoyl-L-tyrosyl-p-aminobenzoic acid or free PABA revealed that PABA is extensively metabolized and conjugated to either p-acetamidobenzoic acid, p-aminohippuric acid, or p-acetamidohippuric acid. PABA concentrations in serum as measured with the Bratton-Marshall ultraviolet spectrophotometric procedure would appear predominantly to reflect measurements of metabolites, with only a minor contribution from PABA itself.

scholarly journals Rapid high performance liquid chromatographic assay for antifungal agents in human sera

1996 ◽  
Vol 37 (3) ◽  
pp. 465-472 ◽  
Author(s):  
T. K. C. Ng ◽  
R. C. Y. Chan ◽  
F. A. B. Adeyemi-Doro ◽  
S. W. Cheung ◽  
A. F. B. Cheng
Keyword(s):  
High Performance ◽  
Antifungal Agents ◽  
High Performance Liquid Chromatographic ◽  
Human Sera ◽  
Liquid Chromatographic

scholarly journals Murine B-cell subpopulations responsive to T-dependent and T-independent antigens.

1976 ◽  
Vol 144 (2) ◽  
pp. 382-397 ◽  
Author(s):  
G K Lewis ◽  
R Ranken ◽  
D E Nitecki ◽  
J W Goodman
Keyword(s):  
B Cells ◽  
B Cell ◽  
Minor Component ◽  
Memory Cell ◽  
Keyhole Limpet Hemocyanin ◽  
The Other ◽  
Other Hand ◽  
Cell Mitogen ◽  
A Minor

Strain A/J mice made secondary indirect plaque-forming cell (PFC) responses to azobenzenearsonate (ABA) conjugates of giant keyhole limpet hemocyanin (KLH), a thymic-dependent antigen, but not to conjugates of Ficoll, a T-independent antigen. ABA-Ficoll was also unable to elicit a response in animals primed with ABA-KLH, which have an expanded anti-ABA memory cell pool. On the other hand, ABA-Ficoll rendered mice unresponsive to ABA-KLH when administered before priming or boosting with the T-dependent immunogen. Hence, the T-independent antigen was able to tolerize but unable to trigger B-memory cells responsive to the T-dependent antigen. A/J mice immunized with dinitrophenyl conjugates of Ficoll or bovine IgG (BGG) made vigorous IgM and IgG PFC responses. PFC responses to ABA-KLH and 2,4-dinitrophenyl (DNP)-BGG were abrogated by depleting mice of C3 with cobra venom factor, whereas the IgM and IgG PFC responses to DNP-Ficoll were unaffected. B lymphocytes were fractionated on the basis of receptors for C3 and the subpopulations were assayed for in vitro PFC responses to DNP-Ficoll. Very little response was obtained from complement receptor lymphocyte [CRL(+)] B cells, whereas CRL(-) cells were more responsive than unfractionated B cells. Both populations responded to a polyclonal B-cell mitogen (lipopolysaccharide). On the other hand, the in vitro PFC response to a T-dependent antigen (sheep erythrocytes) correlated with the presence of CRL(+) B cells in the cultures. However, a minor component of this response, sensitive to anti-Thy-1 serum, was made by CRL(-) B cells, indicating the existence of subpopulations of T-dependent B cells with different signalling requirements. The results suggest that most B cells responsive to T-dependent antigens possess receptors for C3 and that C3 plays an obligatory role in the response of these cells. A distinct subpopulation of B cells which lack C3 receptors respond to T-independent antigens. The precursors of PFC for the ABA epitope reside largely or exclusively in the CRL(+) compartment in A/J mice, whereas precursors for the DNP determinant are found in both compartments.

scholarly journals Authentication of Straight Whiskey by Determination of the Ratio of Furfural to 5-Hydroxymethyl-2-Furaldehyde

1999 ◽  
Vol 82 (4) ◽  
pp. 997-1001 ◽  
Author(s):  
James Jaganathan ◽  
Sumer M Dugar
Keyword(s):  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
The Other ◽  
Color Intensity ◽  
Other Hand ◽  
Liquid Chromatographic Method ◽  
Oak Barrels ◽  
Liquid Chromatographic

Abstract Bureau of Alcohol, Tobacco and Firearms regulations require that a straight whiskey be aged in a freshly charred oak barrel for a minimum of 2 years and that it not be colored with added caramel. The regulations, however, permit addition of caramel in blended whiskeys. Blended whiskeys are usually produced by mixing a straight whiskey with neutral spirits which causes loss of color intensity. Caramel addition is permitted to compensate for this loss. Thus, it is not possible to authenticate the standard of identity of a straight whiskey by measurement of color intensity. Our investigations suggest that furfural (2-furaldehyde) and 5-hydroxymethyl-2-furaldehyde are imparted into a straight whiskey during aging in a freshly charred oak barrel. Caramel, on the other hand, imparts only 5-hydroxymethyl-2-furaldehyde. Thus, the measurement of the concentrations of furfural and 5-hydroxymethyl-2-furaldehyde and their ratio could effectively authenticate the standard of identity of straight whiskeys. This study shows that straight whiskeys aged in freshly charred oak barrels for a period of 2 years or more have a 2:1 or higher ratio of furfural to 5-hydroxymethyl-2-furaldehyde. A high-performance liquid chromatographic method for the determination of furfural and 5-hyroxy- methyl-2-furaldehyde at low parts-per-million levels is described.

scholarly journals Covariation of Ergot Severity and Alkaloid Content Measured by HPLC and One ELISA Method in Inoculated Winter Rye across Three Isolates and Three European Countries

Toxins ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 676
Author(s):  
Anna Kodisch ◽  
Michael Oberforster ◽  
Armin Raditschnig ◽  
Bernd Rodemann ◽  
Anna Tratwal ◽  
...  
Keyword(s):  
High Performance ◽  
Large Deviation ◽  
Ergot Alkaloids ◽  
Minor Component ◽  
Winter Rye ◽  
Screening Assay ◽  
Reliable Prediction ◽  
Grain Elevators ◽  
Food And Feed ◽  
A Minor

Ergot caused by Claviceps purpurea is a problem for food and feed security in rye due to the occurrence of toxic ergot alkaloids (EAs). For grain elevators and breeders, a quick, easy-to-handle, and cheap screening assay would have a high economic impact. The study was performed to reveal (1) the covariation of ergot severity (= percentage of sclerotia in harvested grain) and the content of 12 EAs determined by high performance liquid chromatography (HPLC) and (2) the covariation between these traits and results of one commercial enzyme linked immunosorbent assays (ELISA). In total, 372 winter rye samples consisting of a diverse set of genotypes, locations from Germany, Austria, and Poland over two years, and three isolates were analyzed. Ergocornine and α-ergocryptine were detected as major EAs. Ergocristinine occurred as a minor component. Claviceps isolates from different countries showed a similar EA spectrum, but different quantities of individual EAs. A moderate, positive covariation between ergot severity and EA content determined by HPLC was observed across two years (r = 0.53, p < 0.01), but large deviation from the regression was detected. ELISA values did neither correlate with the HPLC results nor with ergot severity. In conclusion, a reliable prediction of the EA content based on ergot severity is, at present, not possible.

The Coloration of Gladiolus I. Survey of Anthocyanins in Petals of Gladiolus

1981 ◽  
Vol 36 (5-6) ◽  
pp. 378-382 ◽  
Author(s):  
Naaman Akavia ◽  
Dieter Strack ◽  
Avner Cohen
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
The Other ◽  
Major Contributor ◽  
Pigment Composition ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Liquid Chromatographic

Abstract The six common anthocyanidins found in Gladiolus petals occur in four types of glycosilation: 3-glucoside, 3-rhamnoglucoside, 3,5-diglucoside, and 3-rhamnoglucoside-5-glucoside. The six monoglucosides appear in minute quantities, whereas any of the other 18 anthocyanins can serve as the major contributor to the coloration of Gladiolus petals. In high performance liquid chromatographic analyses of petal pigment composition of nine cultivars, it was found that the anthocyanins are grouped on the basis of the aglycon substitution. Thus, pelargonidin appears by itself (group I), cyanidin and peonidin constitute group II, and delphinidin, petunidin, and malvidin group III.

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