Cell Cycle Regulators in Adult Stem Cells

2009 ◽  
pp. 81-87
Author(s):  
Tao Cheng ◽  
David T. Scadden
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Adult Stem Cells ◽  
Cell Cycle Regulators

scholarly journals Quiescent, Slow-Cycling Stem Cell Populations in Cancer: A Review of the Evidence and Discussion of Significance

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Nathan Moore ◽  
Stephen Lyle
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Adult Stem Cells ◽  
Cell Populations ◽  
Proliferative Potential ◽  
Cell Cycle Regulators ◽  
Slow Cycling ◽  
Stem Cell Populations

Long-lived cancer stem cells (CSCs) with indefinite proliferative potential have been identified in multiple epithelial cancer types. These cells are likely derived from transformed adult stem cells and are thought to share many characteristics with their parental population, including a quiescent slow-cycling phenotype. Various label-retaining techniques have been used to identify normal slow cycling adult stem cell populations and offer a unique methodology to functionally identify and isolate cancer stem cells. The quiescent nature of CSCs represents an inherent mechanism that at least partially explains chemotherapy resistance and recurrence in posttherapy cancer patients. Isolating and understanding the cell cycle regulatory mechanisms of quiescent cancer cells will be a key component to creation of future therapies that better target CSCs and totally eradicate tumors. Here we review the evidence for quiescent CSC populations and explore potential cell cycle regulators that may serve as future targets for elimination of these cells.

scholarly journals Single-cell RNA-seq reveals a concomitant delay in differentiation and cell cycle of aged hematopoietic stem cells

BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Léonard Hérault ◽  
Mathilde Poplineau ◽  
Adrien Mazuel ◽  
Nadine Platet ◽  
Élisabeth Remy ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cell Cycle Regulators ◽  
Potential Mechanism ◽  
Rna Seq ◽  
Hematopoietic Stem ◽  
Age Related ◽  
Undifferentiated State ◽  
Reference Map

Abstract Background Hematopoietic stem cells (HSCs) are the guarantor of the proper functioning of hematopoiesis due to their incredible diversity of potential. During aging, heterogeneity of HSCs changes, contributing to the deterioration of the immune system. In this study, we revisited mouse HSC compartment and its transcriptional plasticity during aging at unicellular scale. Results Through the analysis of 15,000 young and aged transcriptomes, we identified 15 groups of HSCs revealing rare and new specific HSC abilities that change with age. The implantation of new trajectories complemented with the analysis of transcription factor activities pointed consecutive states of HSC differentiation that were delayed by aging and explained the bias in differentiation of older HSCs. Moreover, reassigning cell cycle phases for each HSC clearly highlighted an imbalance of the cell cycle regulators of very immature aged HSCs that may contribute to their accumulation in an undifferentiated state. Conclusions Our results establish a new reference map of HSC differentiation in young and aged mice and reveal a potential mechanism that delays the differentiation of aged HSCs and could promote the emergence of age-related hematologic diseases.

scholarly journals The Wnt Signaling Inhibitor Dickkopf-1 Is Required for Reentry into the Cell Cycle of Human Adult Stem Cells from Bone Marrow

2003 ◽  
Vol 278 (30) ◽  
pp. 28067-28078 ◽  
Author(s):  
Carl A. Gregory ◽  
Harpreet Singh ◽  
Anthony S. Perry ◽  
Darwin J. Prockop
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Wnt Signaling ◽  
Adult Stem Cells ◽  
Human Adult ◽  
Dickkopf 1

Negative cell-cycle regulators cooperatively control self-renewal and differentiation of haematopoietic stem cells

2005 ◽  
Vol 7 (2) ◽  
pp. 172-178 ◽  
Author(s):  
Carl R. Walkley ◽  
Matthew L. Fero ◽  
Wei-Ming Chien ◽  
Louise E. Purton ◽  
Grant A. McArthur
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Cell Cycle Regulators ◽  
Self Renewal ◽  
Haematopoietic Stem Cells ◽  
Negative Cell

RELB Alters Proliferation of Human Pluripotent Stem Cells via IMP3- and LIN28-Mediated Modulation of the Expression of IGF2 and Other Cell-Cycle Regulators

2015 ◽  
Vol 24 (16) ◽  
pp. 1888-1900 ◽  
Author(s):  
Nilay Yogeshkumar Thakar ◽  
Dmitry Alexander Ovchinnikov ◽  
Marcus Lachlan Hastie ◽  
Jeffrey Gorman ◽  
Ernst Jurgen Wolvetang
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Cell Cycle Regulators

scholarly journals Sustained PU.1 Levels Balance Cell-Cycle Regulators to Prevent Exhaustion of Adult Hematopoietic Stem Cells

2013 ◽  
Vol 49 (5) ◽  
pp. 934-946 ◽  
Author(s):  
Philipp B. Staber ◽  
Pu Zhang ◽  
Min Ye ◽  
Robert S. Welner ◽  
César Nombela-Arrieta ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cell Cycle Regulators ◽  
Hematopoietic Stem

scholarly journals RNA polymerase II pausing regulates a quiescence-dependent transcriptional program, priming cells for cell cycle reentry

2018 ◽  
Author(s):  
Hardik P. Gala ◽  
Debarya Saha ◽  
Nisha Venugopal ◽  
Ajoy Aloysius ◽  
Jyotsna Dhawan
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Rna Polymerase Ii ◽  
Transcriptional Activation ◽  
Gene Networks ◽  
Adult Stem Cells ◽  
Transcriptional Networks ◽  
Elongation Complex ◽  
Pol Ii ◽  
Pol Ii Pausing

AbstractAdult stem cells persist in mammalian tissues by entering a state of reversible arrest or quiescence associated with low transcription. Using cultured myoblasts and primary muscle stem cells, we show that RNA synthesis is strongly repressed in G0, returning within minutes of activation. We investigate the underlying mechanism and reveal a role for promoter-proximal RNAPol II pausing: by mapping global Pol II occupancy using ChIP-seq, in conjunction with RNA-seq to identify repressed transcriptional networks unique to G0. Strikingly, Pol II pausing is enhanced in G0 on genes encoding regulators of RNA biogenesis (Ncl, Rps24, Ctdp1), and release of pausing is critical for cell cycle re-entry. Finally, we uncover a novel, unexpected repressive role of the super-elongation complex component Aff4 in G0-specific stalling. We propose a model wherein Pol II pausing restrains transcription to maintain G0, preconfigures gene networks required for the G0-G1 transition, and sets the timing of their transcriptional activation.

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