1988 ◽  
Vol 55 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Robert A. Collins ◽  
Keith R. Parsons ◽  
Terry R. Field ◽  
A. John Bramley

SummaryXanthine oxidase (XO) was demonstrated to be present in the teat canal and secretory tissue of the bovine mammary gland by histochemical techniques. Homogenates of these tissues were able to replace XO in an antibacterial assay with Streptococcus uberis. The action of XO on its substrate hypoxanthine was shown to provide an essential component for anti-streptococcal activity mediated by lactoperoxidase. A mechanism is proposed whereby the interaction of XO, lactoperoxidase and thiocyanate may provide antibacterial activity in the teat canal.


2016 ◽  
Vol 49 (2) ◽  
pp. 67-85 ◽  
Author(s):  
M.A.M. Elhamahmy ◽  
M.F. Mahmoud ◽  
T.Y. Bayoumi

Abstract Insect damage in canola adversely affects its productivity andquality and is considered one of the most important degrading factors in Egypt. The effect of foliar application of salicylic acid (SA) on aphid populations, growth and yield of canola (Brassica napus, L.) cv. serw 4 was the major goal of this study. Two experiments were conducted at the farm of Faculty of Agriculture, Suez Canal University, Ismailia, Egypt, during 2014 and 2015 seasons, to achieve this target. Each experiment included four levels of SA (0, 50, 100, 200 mg 1-1). The experimental results revealed that SA, at low concentration (50 mg 1-1), was an effective treatment for reduction the number of aphid populations and colony depth on the main inflorescence, contributed with reducing the thickness of secretory tissue of flower pedicel. The level of 50 mg 1-1 of SA-treated canola had the highest number of stomata cm-2, along with the lowest width of both stoma and its aperture. Thickness of xylem tissue and the number of xylem vessels bundle-1 in leaf midrib, reducing sugars and free amino acids was increased at 50 mg 1-1 SA, but free phenolics content did not affected significantly. Under controlled conditions, changes in temperature of infected leaves allowed the discrimination between healthy and infected areas in thermo-image, even before visible symptoms of aphid infestation appeared. The detection of modifications in plants or canopies, associated with low insect severity in the early stages of infestation, was crucial for the targeted, site-specific or on demand application of integrated aphid control. Canola, which was treated with 50 mg 1-1 of SA, gave 30.5 and 27.9 kg of oil ha-1 over the control. It was concluded that spraying of SA at 50 mg 1-1 was an effective elicitor to diminish the aphid numbers on canola inflorescence and improve its yield.


Steroids ◽  
2013 ◽  
Vol 78 (10) ◽  
pp. 973-981 ◽  
Author(s):  
L. Yart ◽  
V. Lollivier ◽  
L. Finot ◽  
J. Dupont ◽  
S. Wiart ◽  
...  
Keyword(s):  

1978 ◽  
Vol 77 (3) ◽  
pp. 827-836 ◽  
Author(s):  
RE Ostlund ◽  
JT Leung ◽  
DM Kipnis

Myosin has been purified from the principal pancreatic islet of catfish, hog salivary gland, and hog pituitary. Use of the protease inhibitor Trasylol (FBA Pharmaceuticals, New York) was essential in the isolation of pituitary myosin. Secretory tissue myosins were very similar to smooth muscle myosin, having a heavy chain of 200,000 daltons and light chains of 14,000 and 19,000 daltons. Salivary gland myosin cross-reacted with antibodies directed toward both smooth muscle myosin and fibroblast myosin, but not with antiskeletal muscel myosin serum. The specific myosin ATPase activity measured in 0.6 M KCl was present. Tissues associated with secretion of hormone granules contained substantial amounts of this ATPase, rat pancreatic islets having 4.5 times that of rat liver. Activation of low ionic strength myosin ATPase by actin could not be demonstrated despite adequate binding of the myosin to muscle actin and elution by MgATP. The myosins were located primarily in the cytoplasm as determined by cell fractionation and were quite soluble in buffers of low ionic strength.


PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11874
Author(s):  
Małgorzata Stpiczyńska ◽  
Magdalena Kamińska ◽  
Kevin L. Davies

Barkeria scandens and B. whartoniana are endangered, endemic taxa from Mexico. They are epiphytes adapted to dry habitats. Since these plants are xerophytic, their flowers were investigated for structural adaptations to nectar secretion. The flowers of both species are structurally similar, and contrary to most claims for the genus, have functional floral nectaries comprising a nectary chamber and a narrow tubular cuniculus. Nectar is present in both these structures, and contains sugars and lipid-like compounds. The nectary tissue is composed of a single-layered epidermis overlying 1–2 layers of subepidermal secretory parenchyma. The outer tangential wall of the epidermal cells is thick and multi-layered, whereas the cuticle, which often shows blistering, is lamellate and possesses micro-channels. Lipid-like material occurs both between the microfibrils of the cell wall and in the micro-channels. Robust secretory tissue, thick cell walls, and lipid-like nectar components limit nectar evaporation. Moreover, the rigidity of the nectary potentially makes it possible for red-flowered B. scandens to switch from entomophily to ornithophily.


Author(s):  
Filippo Casoni ◽  
Laura Croci ◽  
Francesca Vincenti ◽  
Paola Podini ◽  
Luca Massimino ◽  
...  

ABSTRACTThe choroid plexus (ChP) is a secretory tissue that produces cerebrospinal fluid (CSF) and secretes it into the ventricular system. CSF flows from the lateral to the third ventricle, and then to the fourth ventricle through the cerebral aqueduct. Recent studies have uncovered new, active roles for this structure in the regulation of neural stem cell maintenance and differentiation into neurons. Zfp423, encoding a Kruppel-type zinc finger transcription factor essential for cerebellar development and mutated in rare cases of cerebellar vermis hypoplasia / Joubert syndrome and other ciliopathies, is expressed in the hindbrain roof plate (RP), from which the IV ventricle ChP arises, and in mesenchymal cells giving rise to the stroma and leptomeninges. Zfp423 mutants display a marked reduction of the hindbrain ChP (hChP), which fails to express key markers of its secretory function and genes implicated in its development and maintenance (Lmx1a, Otx2). The mutant hChP displays a complete lack of multiciliated ependymal cells. A transcriptome analysis conducted at the earliest stages of hChP development and subsequent validations demonstrate that the mutant hChp displays a strong deregulation of pathways involved in early hindbrain patterning and multiciliated cell fate specification. Our results propose Zfp423 as a master gene and one of the earliest known determinants of hChP development.


2014 ◽  
Vol 59 (1-4) ◽  
pp. 3-8 ◽  
Author(s):  
Karuppanan Arumugasamy ◽  
Ramalingam B. Subramanian ◽  
Jayant A. Inamdar

The mature cyathial nectary of <i>Euphorbia heterophylla</i> L. consists of distint secretory and sub-secretory zones. The secretory zone consists of 1-3 layer of palisade-like epidermal cells, supplied by both phloem and xylem. Distinct 'secretory pits' surrounded by thin cuticle are present in the secretory tissue. Enzymes such as succinic dehydrogenase and acid phosphatase were localized. Probable functions of cyathial nectaries and animal visitors are discussed.


2012 ◽  
Vol 64 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Magdalena Kamińska ◽  
Małgorzata Stpiczyńska

To date, the structure of the nectary spur of <i>Dendrobium finisterrae</i> has not been studied in detail, and the present paper compares the structural organization of the floral nectary in this species with the spurs of other taxa. The nectary spur of <i>D. finisterrae</i> was examined by means of light microscopy (LM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). It is composed of a single layer of secretory epidermis and several layers of small and compactly arranged subepidermal secretory cells. The secretory cells have thick cellulosic cell walls with primary pits. The secretory tissue is supplied by vascular bundles that run beneath in ground parenchyma and are additionally surrounded by strands of sclerenchymatous fibers. The flowers of the investigated species displayed morphological features characteristic of bee-pollinated taxa, as they are zygomorphic, creamy-green coloured with evident nectar guides. They also emit a weak but nice scent. However, they possess some characters attributed to bird-pollinated flowers such as a short, massive nectary spur and collenchymatous secretory tissue that closely resembles the one found in the nectaries of certain species that are thought to be bird-pollinated. This similarity in anatomical organization of the nectary, regardless of geographical distribution and phylogeny, strongly indicates convergence and appears to be related to pollinator-driven selection.


1955 ◽  
Vol 6 (1) ◽  
pp. 145 ◽  
Author(s):  
HG Turner

Seven cows were each milked at a variety of intervals between milkings a t each of several stages of lactation, intervals being long enough for maximum attainable yields to be approximated. The udder was emptied thoroughly at the beginning and end of each interval with the aid of injections of posterior pituitary extract. Intra-mammary pressure was estimated at. each milking. Net capacity of the udder, as measured by maximum yield, declined during lactation in proportion to the concurrent decline in daily yield, so that any given long interval between milkings reduced yield to almost the same degree in late lactation as in early lactation. The decline in maximum yield or "physiological capacity" was contributed to jointly by decline in 'physical capacity' (volume contained at given intra-mammary pressure) and decline in maximum pressure or secretion pressure. In early lactation, decline in secretion pressure contributed most to decline in physiological capacity, loss of physical capacity becoming important only in late lactation. It appeared that high rate of physical involution was associated with low functional activity of secretory tissue, as measured by secretion pressure, rather than with advanced lactation per se. It is postulated that the physical and functional components of physiological capacity are paralleled by physical and functional components of daily yield, namely, amount of secretory tissue and secretion rate per unit of tissue. Accordingly, decline in secretory intensity contributes primarily to early decline in daily yield, loss of secretory tissue becoming important in late lactation or in cows in which functional activity of secretory tissue is low. Cows appear to differ in the relative contributions of mammary development and functional activity to lactational performance. The pattern and course of involution during lactation may be partly determined by these initial differences. The effect of length of interval between milkings upon secretion is discussed, with emphasis on the difference between immediate and long-term effects. It is pointed out that differences between cows in sensitivity of secretion to long intervals are morphologically rather than functionally determined.


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