TSAb AND T LYMPHOCYTES SUBSET CHANGES IN PATIENTS WITH GRAVES' DISEASE (GD) TREATED BY CARBIMAZOLE (C)

1985 ◽  
pp. 419-420 ◽  
Author(s):  
A.M. Madec ◽  
J. Orgiazzi ◽  
A. Stefanutti ◽  
N. Genetet ◽  
B. Genetet ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Graves Disease

scholarly journals T-lymphocytes phenotypic composition of peripheral blood in patients with Graves’ disease undergoing conservative therapy with thiamazole

2021 ◽  
Vol 67 (6) ◽  
pp. 39-49
Author(s):  
M. A. Dudina ◽  
S. A. Dogadin ◽  
A. A. Savchenko ◽  
V. D. Belenyuk
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Controlled Trial ◽  
Effective Control ◽  
Control Group ◽  
Direct Immunofluorescence ◽  
Graves Disease ◽  
Open Label ◽  
T Helpers

BACKGROUND: Effective control of autoimmune inflammation in Graves’ disease determines necessity to study the T helper (Th) and cytotoxic T-lymphocytes dysfunction, as well as the level of regulatory T-cells (Treg) activation in patients with Graves’ disease on thyrostatic medication, which will clarify the immunomodulatory effects of long-term thiamazole treatment serve as targets for more specific therapies.AIM: To study the phenotypic composition of T-lymphocytes in the peripheral blood of patients with Graves’ disease to assess the direction of immune response depending on thimazole-induced euthyroidism duration.MATERIALS AND METHODS: A single-center, cohort, continuous, open-label, controlled trial was conducted to assess the phenotypic composition of T-lymphocytes in peripheral blood in women with Graves’ disease on long-term thiamazole treatment. The phenotypic composition of T-lymphocytes was determined by flow cytometry using direct immunofluorescence with conjugated FITC monoclonal antibodies depending on the duration of thimazole-induced euthyroidism of long-term thiamazole treatment.RESULTS: The study included 135 women with Graves’ disease, mean age 43.09±12.81 years, 120 (88.91%) with a relapse of the disease and 15 (11.09%) with newly diagnosed hyperthyroidism. An increase of activated CD3+CD4+CD25+ was found in patients with Graves’ disease with a duration of thimazole-induced euthyroidism 5–8 months and 9–12 months, respectively, Me=0.94 (0.48–1.45), p=0.020) and Me=0.95 (0.41–1.80), p=0.025), in control group — Me=0.12 (0.03–0.68). Compared to the control an increase of CD4+CD25+CD127Low (Treg) was found in patients with a duration of thimazole-induced euthyroidism 5–8 and 9–12 months. The content of Treg in peripheral blood in Graves’ disease patients with a duration of thimazole-induced euthyroidism more than 12 months decreases, but remains elevated relative to the control.CONCLUSION: In patients with Graves’ disease with a duration of thimazole-induced euthyroidism 5–8 months and 9–12 months the level of Treg has been increased. The increase of activated Th (CD3+CD4+CD25+) persists independently of thimazole-induced euthyroidism. In patients with Graves’ disease with a duration of thimazole-induced euthyroidism for more than 12 months, there is a compensatory increase in regulatory T-lymphocyte, and the total number of T-helpers is restored to the control.

Predominant intraepithelial localization of primed T cells and immunoglobulin-producing lymphocytes in Graves' disease

1991 ◽  
Vol 124 (6) ◽  
pp. 630-636 ◽  
Author(s):  
R. Paschke ◽  
N. Brückner ◽  
R. Schmeidl ◽  
P. Pfiester ◽  
K. H. Usadel
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
Lymphocytic Infiltration ◽  
Intraepithelial Lymphocytes ◽  
Pokeweed Mitogen ◽  
Graves Disease ◽  
Immunoglobulin Synthesis ◽  
Thyroid Glands ◽  
Staining Index

Abstract. It has been proposed that intrathyroidal lymphocytes, localized in specific anatomical sites might have distinct, pathophysiologically relevant functions in Graves' disease. However, most studies of intrathyroidal lymphocytes were restricted to two lymphocyte locations and used semiquantitative methods. Therefore we used seven anatomically different lymphoid compartments to classify and evaluate by quantitative representative methods the total intrathyroidal lymphocytic infiltration and the staining indexes for immunoglobulin-producing plasmocytes and primed T cells (CD45RO), which provide maximum help to pokeweed mitogen-stimulated immunoglobulin synthesis in 36 thyroid glands from patients with Graves' disease. We found only 3.4% of all intrathyroidal lymphocytes intraepithelially. However, only intraepithelial lymphocytes showed a significantly higher staining index for primed T cells compared with several other compartments. There was also a high staining index for immunoglobulin-producing lymphocytes in this compartment. Kappa- and lambda-positive plasmocytes were found in a polyclonal distribution (kappa:lambda=64.1: 35.9) in all compartments. This increased incidence of CD45RO-positive T lymphocytes and of immunoglobulin-producing lymphocytes among the intraepithelial lymphocytes suggests a distinct pathophysiological function of lymphocytes in peripolesis in Graves' disease. Furthermore, there is a polyclonal intrathyroidal immunoglobulin synthesis.

scholarly journals Treatment of Graves’ disease with methimazole in children alters the proliferation of Treg cells and CD3+ T lymphocytes

2014 ◽  
Vol 52 (1) ◽  
pp. 69-77 ◽  
Author(s):  
Maria Klatka ◽  
Lucyna Kaszubowska ◽  
Ewelina Grywalska ◽  
Magdalena Wasiak ◽  
Leszek Szewczyk ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Treg Cells ◽  
Graves Disease

Altered balance of immunoregulatory T lymphocyte subsets in autoimmune thyroid diseases

1984 ◽  
Vol 105 (2) ◽  
pp. 200-204 ◽  
Author(s):  
Takashi Misaki ◽  
Junji Konishi ◽  
Yasuhiro Iida ◽  
Keigo Endo ◽  
Kanji Torizuka
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
Hashimoto’S Thyroiditis ◽  
Mononuclear Cells ◽  
Cytotoxic T Cells ◽  
Hashimoto's Thyroiditis ◽  
Graves Disease ◽  
Autoimmune Thyroid Diseases ◽  
Autoimmune Thyroid ◽  
Significant Difference

Abstract. Three monoclonal antibodies recognizing cell surface antigens of total peripheral (OKT3), helper/inducer (OKT4) and suppressor/cytotoxic (OKT8) T lymphocytes were used by an indirect immunofluorescence technique to enumerate peripheral T lymphocytes in 25 patients with Graves' disease (including 4 euthyroid Graves' patients), 16 patients with Hashimoto's thyroiditis and 22 normal controls. Total lymphocyte count and percentages of overall T and helper/inducer T cells among peripheral lymphocytes in these conditions showed no significant difference from those of the controls. Percentage of suppressor/cytotoxic T cells, however, was decreased in Graves' disease patients with or without hyperthyroidism. The ratio of helper/inducer T cells to suppressor/cytotoxic T cells was increased in Graves' disease population and slightly increased in hypothyroid Hashimoto's thyroiditis patients. The ratio correlated with the mitogenic response of peripheral mononuclear cells to phytohaemagglutinin, but not with the serum levels of thyroid hormones nor with the titres of thyroid autoantibodies. These findings are in accordance with the results of previous functional studies and indicate possible defects in suppressor T lymphocytes in autoimmune thyroid disease.

Endocrine ophthalmopathy: state-of-the-art approaches

2013 ◽  
Vol 58 (6) ◽  
pp. 24-32 ◽  
Author(s):  
N. A. Petunina ◽  
L. V. Trukhina ◽  
N. S. Martirosyan
Keyword(s):  
T Lymphocytes ◽  
Autoimmune Thyroiditis ◽  
Thyroid Dysfunction ◽  
State Of The Art ◽  
Clinical Manifestations ◽  
Eye Disease ◽  
Graves Disease ◽  
Challenging Problem ◽  
Chronic Autoimmune Thyroiditis ◽  
Endocrine Ophthalmopathy

The issues pertaining to etiology, pathogenesis, clinical manifestations, and treatment of endocrine ophthalmopathy (EOP) are discussed. EOP is a heterogeneous autoimmune eye disease most frequently associated with Graves’ disease even though it is just as well encountered both in the patients presenting with chronic autoimmune thyroiditis and in the absence of thyroid dysfunction. Although pathogenesis of EOP remains to be elucidated its autoimmune nature with the involvement of sensitized T-lymphocytes and autoantibodies against orbital tissues leaves no doubt. The understanding of mechanisms underlying the development of EOP gave impetus to the creation of new groups of medicines selectively acting on various pathogenic processes associated with this disease. The management of EOP remains a challenging problem requiring a multi-disciplinary approach for its solution.

Decrease of Immunoglobulin G-Fc Receptor-Bearing T Lymphocytes in Graves' Disease*

1982 ◽  
Vol 55 (3) ◽  
pp. 399-402 ◽  
Author(s):  
HIDEMITSU MORI ◽  
NOBUYUKI AMINO ◽  
YOSHINORI IWATANI ◽  
SEISHI ASARI ◽  
YASUYUKI IZUMIGUCHI ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Immunoglobulin G ◽  
Fc Receptor ◽  
Graves Disease
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