Establishment of a 2-week canine skin organ culture model and its pharmacological modulation by epidermal growth factor and dexamethasone

2016 ◽  
Vol 207 ◽  
pp. 109-117 ◽  
Author(s):  
Francesca Abramo ◽  
Andrea Pirone ◽  
Carla Lenzi ◽  
Iacopo Vannozzi ◽  
Maria Federica della Valle ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Organ Culture ◽  
Pharmacological Modulation ◽  
Culture Model ◽  
Epidermal Growth ◽  
Skin Organ Culture

scholarly journals The effect of epidermal growth factor (EGF) on the differentiation of the rough endoplasmic reticulum in fetal mouse small intestine in organ culture.

1981 ◽  
Vol 29 (6) ◽  
pp. 765-770 ◽  
Author(s):  
J F Beaulieu ◽  
R Calvert
Keyword(s):  
Endoplasmic Reticulum ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Organ Culture ◽  
Rough Endoplasmic Reticulum ◽  
Fetal Mouse ◽  
Absorptive Cells ◽  
Mouse Small Intestine ◽  
Epidermal Growth ◽  
Mouse Fetuses

The differentiation of the rough endoplasmic reticulum (RER) of mouse duodenal absorptive cells located at the tip of the villi at 17 days of gestation was compared to that of absorptive cells in duodenal explants of 15-day-old mouse fetuses cultured for 72 hr 1) with Trowell T8 medium (without insulin) alone or supplemented 2) with epidermal growth factor (EGF; 100 ng/ml) or 3) with 25% bovine amniotic fluid (BAF). Glucose-6-phosphatase activity (G6Pase) was localized cytochemically to ensure a better identification of the RER. The intersections of a double lattice falling over and outside the RER were counted and the percentage of intersections over the RER was estimated. With this method, the extent of the RER is not statistically different when the absorptive cells in utero are compared to those of explants cultured with EGF. However, the extent of the RER in the absorptive cells cultured with Trowell T8 medium alone or supplemented with BAF is 50% lower than in the former two groups. It is concluded that EGF promotes the maturation of duodenal absorptive cells in organ culture.

scholarly journals Use of a Serum-Free Epidermal Culture Model to Show Deleterious Effects of Epidermal Growth Factor on Morphogenesis and Differentiation

1995 ◽  
Vol 104 (1) ◽  
pp. 107-112 ◽  
Author(s):  
Chih-Shan J. Chen ◽  
Robert M. Lavker ◽  
Ulrich Rodeck ◽  
Barbara Risse ◽  
Pamela J. Jensen
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Serum Free ◽  
Culture Model ◽  
Epidermal Growth

Epidermal Growth Factor Stimulates Synthesis and Secretion of Mucus Glycoproteins in Human Gastric Mucosa

1990 ◽  
Vol 79 (5) ◽  
pp. 425-427 ◽  
Author(s):  
S. M. Kelly ◽  
J. O. Hunter
Keyword(s):  
Growth Factor ◽  
Gastric Mucosa ◽  
Epidermal Growth Factor ◽  
Organ Culture ◽  
Human Gastric Mucosa ◽  
Main Constituent ◽  
Mucus Production ◽  
Gastric Cytoprotection ◽  
Epidermal Growth ◽  
Mucus Glycoproteins

1. The effects of epidermal growth factor on the synthesis and secretion by human gastric mucosa of radiolabelled mucus glycoprotein have been studied in organ culture. 2. Addition of epidermal growth factor at a concentration of 5 or 10 ng/ml increased the synthesis of glycoprotein from 84 ± 16.2 d.p.m./μg of protein to 132 ± 19.5 and 156 ±20.2 d.p.m./μg of protein, respectively. The same doses increased secretion from 73.75 ± 17 d.p.m./μg of protein to 114.0 ± 26.4 and 141.5 ±31.4 d.p.m./μg of protein (P < 0.001). 3. As glycoproteins are the main constituent of the mucus defence barrier, epidermal growth factor-stimulated mucus production may contribute to gastric cytoprotection.

Sign in / Sign up

Export Citation Format

Share Document