Beneficial effect of Curcumin Nanoparticles-Hydrogel on excisional skin wound healing in type-I diabetic rat: Histological and immunohistochemical studies

Skin wound healing is a complex and dynamic process that aims to restore lesioned tissues. Collagen-based skin substitutes are a promising treatment to promote wound healing by mimicking the native skin structure. Recently, collagen from marine organisms has gained interest as a source for producing biomaterials for skin regenerative strategies. This preliminary study aimed to describe the application of a collagen-based skin-like scaffold (CBSS), manufactured with collagen extracted from sea urchin food waste, to treat experimental skin wounds in a large animal. The wound-healing process was assessed over different time points by the means of clinical, histopathological, and molecular analysis. The CBSS treatment improved wound re-epithelialization along with cell proliferation, gene expression of growth factors (VEGF-A), and development of skin adnexa throughout the healing process. Furthermore, it regulated the gene expression of collagen type I and III, thus enhancing the maturation of the granulation tissue into a mature dermis without any signs of scarring as observed in untreated wounds. The observed results (reduced inflammation, better re-epithelialization, proper development of mature dermis and skin adnexa) suggest that sea urchin-derived CBSS is a promising biomaterial for skin wound healing in a “blue biotechnologies” perspective for animals of Veterinary interest.

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EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS

ABCD Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) ◽

10.1590/0102-672020190001e1498 ◽

2020 ◽

Vol 33 (1) ◽

Author(s):

Letícia Fuganti CAMPOS ◽

Eliane TAGLIARI ◽

Thais Andrade Costa CASAGRANDE ◽

Lúcia de NORONHA ◽

Antônio Carlos L. CAMPOS ◽

...

Keyword(s):

Diabetes Mellitus ◽

Wound Healing ◽

Type I Collagen ◽

Chronic Wounds ◽

Skin Wound ◽

Diabetic Rats ◽

Control Group ◽

Type I ◽

Collagen Deposition ◽

Skin Wound Healing

ABSTRACT Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

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Platelet rich plasma–complexed hydrogel glue enhances skin wound healing in a diabetic rat model

BIOCELL ◽

10.32604/biocell.2022.015592 ◽

2022 ◽

Vol 46 (5) ◽

pp. 1329-1338

Author(s):

YUNLONG ZHANG ◽

JINGWEI ZHANG ◽

YU ZHU ◽

BIN CAI

Keyword(s):

Wound Healing ◽

Rat Model ◽

Platelet Rich Plasma ◽

Skin Wound ◽

Diabetic Rat ◽

Skin Wound Healing ◽

Diabetic Rat Model

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Effect of SACCHACHITIN on keratinocyte proliferation and the expressions of type I collagen and tissue-transglutaminase during skin wound healing

Journal of Biomedical Materials Research ◽

10.1002/jbm.b.30028 ◽

2004 ◽

Vol 70B (1) ◽

pp. 122-129 ◽

Cited By ~ 5

Author(s):

Wei-Sheng Hung ◽

Wen-Fu T. Lai ◽

Bowen Leu ◽

Ching-Hua Su ◽

Chia-Lang Fang ◽

...

Keyword(s):

Wound Healing ◽

Type I Collagen ◽

Tissue Transglutaminase ◽

Skin Wound ◽

Type I ◽

Skin Wound Healing ◽

Keratinocyte Proliferation

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The effect of combined photobiomodulation and curcumin on skin wound healing in type I diabetes in rats

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2018.02.023 ◽

2018 ◽

Vol 181 ◽

pp. 23-30 ◽

Cited By ~ 15

Author(s):

Hasan Soleimani ◽

Abdollah Amini ◽

Soudabeh Taheri ◽

Ensieh Sajadi ◽

Sasha Shafikhani ◽

...

Keyword(s):

Wound Healing ◽

Type I Diabetes ◽

Skin Wound ◽

Type I ◽

Skin Wound Healing

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Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

Planta Medica ◽

10.1055/a-1101-9326 ◽

2020 ◽

Vol 86 (05) ◽

pp. 348-355 ◽

Cited By ~ 1

Author(s):

Soo Min Park ◽

Kyung Jong Won ◽

Dae Il Hwang ◽

Do Yoon Kim ◽

Ha Bin Kim ◽

...

Keyword(s):

Wound Healing ◽

Human Fibroblasts ◽

Skin Wound ◽

Skin Regeneration ◽

Mitogen Activated Protein Kinase ◽

Boyden Chamber ◽

Type I ◽

Skin Wound Healing ◽

Beneficial Effects ◽

Ms Analysis

Abstract Digitaria ciliaris is widely reported to be a problematic weed in agricultural areas and is mainly used as an indicator plant for the development of herbicides. However, its bioactivities on skin regeneration and wound healing have not been investigated. In the present study, we investigated the effects of D. ciliaris flower absolute on skin wound healing and skin regeneration-related events, that is, proliferation, migration, and collagen biosynthesis, in human fibroblasts and keratinocytes. For this study, we extracted absolute from the D. ciliaris flower by solvent extraction and identified the composition of D. ciliaris flower absolute using GC/MS analysis. We also tested the effect of D. ciliaris flower absolute in CCD986sk fibroblasts and/or HaCaT keratinocytes using the WST assay and 5-bromo-2′-deoxyuridine incorporation assay, Boyden chamber assay, ELISA, sprouting assay, and immunoblotting. GC/MS analysis of D. ciliaris flower absolute revealed that it contained 15 compounds. The absolute increased the proliferations of keratinocytes and fibroblasts and the migration of fibroblasts but did not affect cell viabilities. In addition, it enhanced the syntheses of type I and IV collagen in fibroblasts, but not in keratinocytes. The sprouting assay showed increased sprout outgrowth of fibroblasts. In addition, D. ciliaris flower absolute induced the phosphorylation of extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase in fibroblasts. These results indicate that D. ciliaris flower absolute may promote skin wound healing/regeneration by inducing the proliferation, migration, and collagen synthesis of fibroblasts, as well as the proliferation of keratinocytes. Therefore, D. ciliaris flower absolute may be a potential natural source for cosmetic or pharmaceutical agents that promote skin wound healing/regeneration.

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Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes

BioMed Research International ◽

10.1155/2017/9538351 ◽

2017 ◽

Vol 2017 ◽

pp. 1-11 ◽

Cited By ~ 9

Author(s):

Mariáurea M. Sarandy ◽

Rômulo D. Novaes ◽

Antônio A. Xavier ◽

Camilo E. Vital ◽

João P. V. Leite ◽

...

Keyword(s):

Wound Healing ◽

Type I Diabetes ◽

Scar Tissue ◽

Skin Wound ◽

Diabetic Rats ◽

Type I ◽

Skin Wound Healing ◽

Experimental Type ◽

Hydroethanolic Extract

The effect of topical application of ointment based on Strychnos pseudoquina hydroethanolic extract in the cutaneous wounds healing in diabetic rats was evaluated. Samples of S. pseudoquina were submitted to phytochemical prospection and in vitro antioxidant assay. Thirty Wistar rats were divided into 5 groups: Sal-wounds treated with 0.9% saline solution; VH-wounds treated with 0.6 g of lanolin cream (vehicle); SS-wounds treated with silver sulfadiazine cream (10 mg/g); ES5- and ES10-wounds treated with an ointment of S. pseudoquina extract, 5% and 10%, respectively. Fragments of wounds were removed for histological and biochemical analysis every 7 days during 21 days. ES showed equivalent levels per gram of extract of total phenols and flavonoids equal to 122.04 mg for TAE and 0.60 mg for RE. The chlorogenic acid was one of the major constituents. S. pseudoquina extract presented high antioxidant potential in vitro. ES5 and ES10 showed higher wound healing rate and higher amount of cells, blood vessels, and type III and I collagen. The oxidative stress markers were lower in the ES5 and ES10 groups, while the antioxidants enzymes levels were higher. Ointment based on S. pseudoquina extract promotes a fast and efficient cutaneous repair in diabetic rats.

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