Adaptor-tagged competitive polymerase chain reaction: amplification bias and quantified gene expression levels

2005 ◽  
Vol 339 (1) ◽  
pp. 15-28 ◽  
Author(s):  
Hiroko Kita-Matsuo ◽  
Naoto Yukinawa ◽  
Ryo Matoba ◽  
Sakae Saito ◽  
Shigeyuki Oba ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Amplification ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Expression Levels ◽  
Amplification Bias ◽  
Reaction Amplification ◽  
Polymerase Chain ◽  
Gene Expression Levels

scholarly journals Involvement of Both Subgroups A and B of Expansin Genes in Kiwifruit Fruit Ripening

HortScience ◽  
2007 ◽  
Vol 42 (2) ◽  
pp. 315-319 ◽  
Author(s):  
Shaolan Yang ◽  
Changjie Xu ◽  
Bo Zhang ◽  
Xian Li ◽  
Kunsong Chen
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Fruit Ripening ◽  
Acid Treatment ◽  
Polymerase Chain Reaction Amplification ◽  
Chain Reaction ◽  
Degenerate Primers ◽  
Complementary Dna ◽  
Reaction Amplification ◽  
Polymerase Chain

Two complementary DNA fragments encoding expansin genes Ad-EXP1 and Ad-EXP2 were isolated from ripening kiwifruit (Actinidia deliciosa cv. Bruno) by reverse transcription–polymerase chain reaction amplification using a pair of degenerate primers. The homology between these two expansin family members was 50% in nucleotide sequence and 74% in amino acid sequence. It was revealed that Ad-EXP1 and Ad-EXP2 belong to subgroups A and B of an expansin gene family respectively. However, gene expression of these two members shared similar patterns. Both were upregulated by ethylene treatment and downregulated by acetylsalicylic acid treatment. The study suggests that members of both subgroups A and B of the expansin family are involved in kiwifruit fruit ripening.

Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression

1999 ◽  
Vol 20 (2) ◽  
pp. 230 ◽  
Author(s):  
Marianne Jorgensen ◽  
Maja Bévort ◽  
Thuri S. Kledal ◽  
Brian V. Hansen ◽  
Marlene Dalgaard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Differential Display ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain
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