A photoaffinity probe designed for host-specific signal flavonoid receptors in phytopathogenic Peronosporomycete zoospores of Aphanomyces cochlioides

2004 ◽  
Vol 432 (2) ◽  
pp. 145-151 ◽  
Author(s):  
Yasuko Sakihama ◽  
Takashi Shimai ◽  
Mitsuyoshi Sakasai ◽  
Toshiaki Ito ◽  
Yukiharu Fukushi ◽  
...  
Keyword(s):  
Aphanomyces Cochlioides ◽  
Specific Signal

scholarly journals Flight-call as species-specific signal in South American parrots and the effect of species relatedness in call similarity

2017 ◽  
Vol 25 (3) ◽  
pp. 143-151 ◽  
Author(s):  
Carlos B. de Araújo ◽  
Paulo A. M. Marques ◽  
Jacques M. E. Vielliard
Keyword(s):  
South American ◽  
Specific Signal ◽  
As Species ◽  
Species Specific

scholarly journals Genotype-Specific Signal Generation Based on Digestion of 3-Way DNA Junctions: Application to KRAS Variation Detection

2006 ◽  
Vol 52 (10) ◽  
pp. 1855-1863 ◽  
Author(s):  
Giulia Amicarelli ◽  
Daniel Adlerstein ◽  
Erlet Shehi ◽  
Fengfei Wang ◽  
G Mike Makrigiorgos
Keyword(s):  
Nucleic Acid ◽  
Concordance Rate ◽  
Signal Generation ◽  
Sequence Variants ◽  
Wild Type ◽  
Specific Signal ◽  
Novel Technology ◽  
Dna Junctions ◽  
Wide Range ◽  
Kras Codon

Abstract Background: Genotyping methods that reveal single-nucleotide differences are useful for a wide range of applications. We used digestion of 3-way DNA junctions in a novel technology, OneCutEventAmplificatioN (OCEAN) that allows sequence-specific signal generation and amplification. We combined OCEAN with peptide-nucleic-acid (PNA)-based variant enrichment to detect and simultaneously genotype v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) codon 12 sequence variants in human tissue specimens. Materials and Methods: We analyzed KRAS codon 12 sequence variants in 106 lung cancer surgical specimens. We conducted a PNA-PCR reaction that suppresses wild-type KRAS amplification and genotyped the product with a set of OCEAN reactions carried out in fluorescence microplate format. The isothermal OCEAN assay enabled a 3-way DNA junction to form between the specific target nucleic acid, a fluorescently labeled “amplifier”, and an “anchor”. The amplifier-anchor contact contains the recognition site for a restriction enzyme. Digestion produces a cleaved amplifier and generation of a fluorescent signal. The cleaved amplifier dissociates from the 3-way DNA junction, allowing a new amplifier to bind and propagate the reaction. Results: The system detected and genotyped KRAS sequence variants down to ∼0.3% variant-to-wild-type alleles. PNA-PCR/OCEAN had a concordance rate with PNA-PCR/sequencing of 93% to 98%, depending on the exact implementation. Concordance rate with restriction endonuclease-mediated selective-PCR/sequencing was 89%. Conclusion: OCEAN is a practical and low-cost novel technology for sequence-specific signal generation. Reliable analysis of KRAS sequence alterations in human specimens circumvents the requirement for sequencing. Application is expected in genotyping KRAS codon 12 sequence variants in surgical specimens or in bodily fluids, as well as single-base variations and sequence alterations in other genes.

Aphanomyces cochlioides. [Distribution map].

2021 ◽  
Author(s):  
Keyword(s):  
Nova Scotia ◽  
North America ◽  
South America ◽  
Sugar Beet ◽  
South Dakota ◽  
Geographical Distribution ◽  
Spinacia Oleracea ◽  
Distribution Map ◽  
Aphanomyces Cochlioides ◽  
New Distribution

Abstract A new distribution map is provided for Aphanomyces cochlioides Drechsler. Peronosporea: Saprolegniales: Leptolegniaceae. Hosts: spinach (Spinacia oleracea), sugar beet (Beta vulgaris), and other members of the Chenopodiaceae and Amaranthaceae. Information is given on the geographical distribution in Africa (Egypt), Asia (Japan, Hokkaido, Turkey), Europe (Austria, Belgium, Bulgaria, Croatia, Denmark, Estonia, France, Germany, Hungary, Ireland, Moldova, Netherlands, Poland, Russia, Spain, Sweden, Ukraine, UK), North America (Canada, Alberta, Nova Scotia, Ontario, Quebec, USA, Arizona, California, Connecticut, Idaho, Indiana, Iowa, Maine, Michigan, Minnesota, Montana, Nebraska, North Dakota, Ohio, South Dakota, Texas, Washington, Wisconsin, Wyoming), Oceania (Australia, Queensland), and South America (Chile).

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