Dual-emission fluorescence biosensing of vancomycin based on AIEgen–peptide conjugates and aptamer-modified Au nanoclusters

2021 ◽  
Vol 1150 ◽  
pp. 238177
Author(s):  
Fangya Mu ◽  
Junqing He ◽  
Fang Fan ◽  
Guoyue Shi
Keyword(s):  
Au Nanoclusters ◽  
Dual Emission ◽  
Peptide Conjugates

Dual-emission fluorescent sensor based on AIE organic nanoparticles and Au nanoclusters for the detection of mercury and melamine

Nanoscale ◽  
2015 ◽  
Vol 7 (18) ◽  
pp. 8457-8465 ◽  
Author(s):  
Caixia Niu ◽  
Qiuling Liu ◽  
Zhehai Shang ◽  
Liu Zhao ◽  
Jin Ouyang
Keyword(s):  
Fluorescent Sensor ◽  
Au Nanoclusters ◽  
Dual Emission ◽  
Organic Nanoparticles

68Ga-labeled HBED-CC Variant of uPAR Targeting Peptide AE105 Compared with 68Ga-NODAGA-AE105

2019 ◽  
Vol 18 (9) ◽  
pp. 1289-1294 ◽  
Author(s):  
Kusum Vats ◽  
Rohit Sharma ◽  
Haladhar D. Sarma ◽  
Drishty Satpati ◽  
Ashutosh Dash
Keyword(s):  
Solid Phase ◽  
Evaluation Studies ◽  
Radiochemical Yield ◽  
In Vivo Evaluation ◽  
Peptide Conjugates ◽  
Biodistribution Studies ◽  
Target Organs ◽  
U87mg Tumor

Aims: The urokinase Plasminogen Activator Receptors (uPAR) over-expressed on tumor cells and their invasive microenvironment are clinically significant molecular targets for cancer research. uPARexpressing cancerous lesions can be suitably identified and their progression can be monitored with radiolabeled uPAR targeted imaging probes. Hence this study aimed at preparing and evaluating two 68Ga-labeled AE105 peptide conjugates, 68Ga-NODAGA-AE105 and 68Ga-HBED-CC-AE105 as uPAR PET-probes. Method: The peptide conjugates, HBED-CC-AE105-NH2 and NODAGA-AE105-NH2 were manually synthesized by standard Fmoc solid phase strategy and subsequently radiolabeled with 68Ga eluted from a commercial 68Ge/68Ga generator. In vitro cell studies for the two radiotracers were performed with uPAR positive U87MG cells. Biodistribution studies were carried out in mouse xenografts with the subcutaneously induced U87MG tumor. Results: The two radiotracers, 68Ga-NODAGA-AE105 and 68Ga-HBED-CC-AE105 that were prepared in >95% radiochemical yield and >96% radiochemical purity, exhibited excellent in vitro stability. In vivo evaluation studies revealed higher uptake of 68Ga-HBED-CC-AE105 in U87MG tumor as compared to 68Ga-NODAGAAE105; however, increased lipophilicity of 68Ga-HBED-CC-AE105 resulted in slower clearance from blood and other non-target organs. The uPAR specificity of the two radiotracers was ascertained by significant (p<0.05) reduction in the tumor uptake with a co-injected blocking dose of unlabeled AE-105 peptide. Conclusion: Amongst the two radiotracers studied, the neutral 68Ga-NODAGA-AE105 with more hydrophilic chelator exhibited faster clearance from non-target organs. The conjugation of HBED-CC chelator (less hydrophilic) resulted in negatively charged 68Ga-HBED-CC-AE105 which was observed to have high retention in blood that decreased target to non-target ratios.

Peptide conjugates of lactoferricin analogues and antimicrobials—Design, chemical synthesis, and evaluation of antimicrobial activity and mammalian cytotoxicity

Peptides ◽  
2019 ◽  
Vol 117 ◽  
pp. 170079 ◽  
Author(s):  
Natalia Ptaszyńska ◽  
Katarzyna Olkiewicz ◽  
Joanna Okońska ◽  
Katarzyna Gucwa ◽  
Anna Łęgowska ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Synthesis ◽  
Peptide Conjugates

Reversible two-channel mechanochromic luminescence for a pyridinium-based white-light emitter with room-temperature fluorescence–phosphorescence dual emission

2019 ◽  
Vol 21 (27) ◽  
pp. 14728-14733 ◽  
Author(s):  
Xiao Ma ◽  
Jipeng Li ◽  
Chensheng Lin ◽  
Guoliang Chai ◽  
Yangbin Xie ◽  
...  
Keyword(s):  
White Light ◽  
Room Temperature ◽  
Mechanical Grinding ◽  
Dual Emission ◽  
Light Emitter ◽  
Mechanical Pressing ◽  
Mechanochromic Luminescence ◽  
White Light Emitter

For a pyridinium-based emitter with fluorescence–phosphorescence dual emission, mechanical grinding induces phosphorescence disappearance and mechanical pressing induces extraordinary phosphorescence enhancement.

scholarly journals Noval Dual-Emission Fluorescence Carbon Dots as a Ratiometric Probe for Cu2+ and ClO− Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1232
Author(s):  
Jiaqing Guo ◽  
Aikun Liu ◽  
Yutian Zeng ◽  
Haojie Cai ◽  
Shuai Ye ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Background Noise ◽  
Good Recovery ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Simple Method ◽  
Real Samples ◽  
Nitrogen Doped ◽  
Probe Concentration ◽  
Ratiometric Probe

The use of carbon dots (CDs) with dual emission based on ratiometric fluorescence has been attracting attention in recent times for more accurate ion detection since they help avoid interference from background noise, probe concentration, and complexity. Herein, novel dual-emission nitrogen-doped CDs (NCDs) were prepared by a simple method for Cu2+ and ClO- detection. The NCDs showed excellent anti-interference ability and selectivity for different emissions. In addition, a good linear relationship was observed between the fluorescence intensity (FI) of the NCD solutions in different emissions with Cu2+ (0–90 μM) and ClO- (0–75 μM). The limits of both Cu2+ detection and ClO- were very low, at 17.7 and 11.6 nM, respectively. The NCDs developed herein also showed a good recovery rate in water for Cu2+ and ClO− detection. Hence, they are expected to have a more extensive application prospect in real samples.

scholarly journals Dual-emission ratio fluorescence for selective and sensitive detection of ferric ions and ascorbic acid based on one-pot synthesis of glutathione protected gold nanoclusters

RSC Advances ◽  
2021 ◽  
Vol 11 (28) ◽  
pp. 17283-17290
Author(s):  
Shuai Zhang ◽  
Cong Zhang ◽  
Xiaodong Shao ◽  
Rentian Guan ◽  
Yingying Hu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Gold Nanoclusters ◽  
Sensitive Detection ◽  
Dual Fluorescence ◽  
Ferric Ions ◽  
Fluorometric Method ◽  
One Pot ◽  
Dual Emission ◽  
One Pot Synthesis

A fluorometric method was proposed for the determination of Fe3+ and ascorbic acid (AA) based on blue and red dual fluorescence emissions of glutathione (GSH) stabilized-gold nanoclusters (AuNCs).

scholarly journals Synthesis and Luminescence Properties of Two-Electron Bimetallic Cu–Ag and Cu–Au Nanoclusters via Copper Hydride Precursors

2021 ◽  
Author(s):  
Rhone P. Brocha Silalahi ◽  
Tzu-Hao Chiu ◽  
Jhen-Heng Kao ◽  
Chun-Yen Wu ◽  
Chi-Wei Yin ◽  
...  
Keyword(s):  
Luminescence Properties ◽  
Copper Hydride ◽  
Au Nanoclusters
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