Selecting specific aptamers that bind to ovine pregnancy-associated glycoprotein 7 using real serum sample–assisted FluMag-SELEX to develop magnetic microparticle–based colorimetric aptasensor

2021 ◽  
pp. 339291
Author(s):  
Chunxia Lu ◽  
Changbin Liu ◽  
Qin Zhou ◽  
Xia Chen ◽  
Hongmin Li ◽  
...  
Keyword(s):  
Serum Sample ◽  
Magnetic Microparticle ◽  
Colorimetric Aptasensor

scholarly journals An overlooked situation in the interpretation of serum thyroglobulin level in a papillary thyroid cancer patient

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Fevziye Burcu Sirin ◽  
Hakan Korkmaz
Keyword(s):  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Serum Sample ◽  
Imaging Techniques ◽  
Needle Aspiration ◽  
Papillary Thyroid ◽  
Serum Samples ◽  
Serum Thyroglobulin ◽  
Thyroid Cancer Patient ◽  
Serial Dilutions

AbstractIn the present study we report a case of thyroglobulin (TGB) measurement interference in a total thyroidectomized and radio-ablated 61-year old woman with papillary thyroid cancer. We investigated possible interference in the measurement of TGB due to discordant TGB in relation to clinical condition during the follow-up period. Serum TGB was measured with the chemiluminescence method using Beckman Coulter Unicel DxI 800 instrument. To investigate possible interference in TGB measurement serial dilutions, polyethylene glycol precipitation (PEG), treatment with heterophile blocking tube (HBT), rheumatoid factor level determination and retesting of TGB with an alternative method were performed. Serial dilutions of the serum sample revealed linearity but a remarkable decrease in TGB in the patient’s serum samples post PEG and post HBT treatments. Also, TGB results under functional sensitivity level obtained with a different method suggested that TGB interference developed due to heterophile antibody presence in the serum sample. The patient had unnecessarily undergone expensive imaging techniques, and invasive procedures such as lymph node fine needle aspiration biopsy, before the analytical interference was suspected by the clinician. This report illustrates the importance of early communication and close collaboration between clinicians and laboratorians in order to avoid unnecessary clinical intervention.

Truncated affinity-improved aptamers for 17β-estradiol determination by AuNPs-based colorimetric aptasensor

2021 ◽  
Vol 340 ◽  
pp. 128181
Author(s):  
Lu Qiao ◽  
He Wang ◽  
Junlin He ◽  
Shuming Yang ◽  
Ailiang Chen
Keyword(s):  
17Β Estradiol ◽  
Colorimetric Aptasensor

A System for Individualized Dosing of Intravenous Aminophylline Using a Programmable Calculator

PEDIATRICS ◽  
1984 ◽  
Vol 73 (1) ◽  
pp. 64-67
Author(s):  
J. Chris Mitsuoka ◽  
Richard J. Fleck
Keyword(s):  
Steady State ◽  
Serum Sample ◽  
Drug Exposure ◽  
Plasma Concentrations ◽  
Dose Administration ◽  
Programmable Calculator ◽  
Concentration Determination ◽  
A Value ◽  
Individualized Dosing ◽  
History Of

A program that calculates a value of clearance for an individual patient prior to reaching steady state in the early stages of aminophylline therapy is presented. The program is written for the Texas Instruments TI-59 programmable calculator and may be used with or without the PC-100C printer. The program can provide clinically useful information concerning projected plasma concentrations prior to reaching steady state with an accurate history of the dose administration and serum concentration determination. If the patient has not received xanthene therapy prior to admission, only one serum sample is required. If there has been prior drug exposure, a second serum sample is required. An iterative technique, which would be impractical to use without calculator assistance, is employed to make these determinations.

scholarly journals False low holotranscobalamin levels in a patient with a novel TCN2 mutation

2016 ◽  
Vol 54 (11) ◽  
Author(s):  
Peter Keller ◽  
Janine Rufener ◽  
Christof Schild ◽  
Sergey N. Fedosov ◽  
Peter H. Nissen ◽  
...  
Keyword(s):  
Point Mutation ◽  
Serum Sample ◽  
Screening Test ◽  
Initial Treatment ◽  
Sanger Sequencing ◽  
Single Point ◽  
Gel Filtration ◽  
Reference Interval ◽  
Single Point Mutation ◽  
Abbott Architect

AbstractBackground:Measurement of holotranscobalamin (holoTC) is increasingly used as a screening test for cobalamin (Cbl) deficiency. A level well below the reference interval strongly supports a deficient state. We examined a 21-year-old woman diagnosed as Cbl deficient because of an extremely low holoTC level as measured by the Abbott Architect Assay.Methods:The patient was evaluated for Cbl deficiency employing an in-house holoTC method as well as other routine markers of Cbl status. Further analyses included exploration of the Cbl binding proteins employing gel filtration of a serum sample saturated with 57 Co-labeled Cbl and Sanger sequencing of exons 1–9 and the intron-exon boundaries of theResults:The patient had normal hematological variables throughout. Despite initial treatment with Cbl, holoTC as measured by the Abbott assay remained low, while holoTC measured with the in-house assay was normal, and behaved as TC upon gel-filtration. By Sanger sequencing, we detected a homozygous single point mutation c.855T>A in exon 6 ofConclusions:We describe a novel point mutation of the

scholarly journals Thrombin-Mediated Degradation of Human Cardiac Troponin T

2017 ◽  
Vol 63 (6) ◽  
pp. 1094-1100 ◽  
Author(s):  
Ivan A Katrukha ◽  
Alexander E Kogan ◽  
Alexandra V Vylegzhanina ◽  
Marina V Serebryakova ◽  
Ekaterina V Koshkina ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Serum Sample ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Specific Inhibitor ◽  
Cardiac Troponin T ◽  
Amino Acid Residues ◽  
Serum Samples ◽  
Heparin Plasma ◽  
Normal Human

AbstractBACKGROUNDCardiac troponin T (cTnT) is an acknowledged biomarker of acute myocardial infarction (AMI) that is known to be prone to proteolytic degradation in serum. Such degradation is usually explained by the action of μ-calpain, although there could be other candidates for that role. In the current study, we explored the hypothesis that thrombin-mediated cTnT cleavage occurs as a result of the serum sample preparation.METHODScTnT degradation was studied by using immunoblotting and mass spectrometry (MS) analysis.RESULTSThe comparison of cTnT isolated from AMI heparin plasma and serum samples showed that cTnT in the plasma samples was mainly present as the full-sized molecule (approximately 35 kDa), while in serum samples it was present as a 29-kDa fragment. The incubation of recombinant cTnT, or native ternary cardiac troponin complex with thrombin or in normal human serum (NHS), resulted in the formation of a 29-kDa product that was similar to that detected in AMI serum samples. No cTnT degradation was observed when thrombin or NHS was pretreated with hirudin, a specific inhibitor of thrombin, or during incubation of troponin in normal heparin plasma. When the products of thrombin-mediated cTnT proteolysis were analyzed by MS, 2 fragments consisting of amino acid residues (aar) 2–68 and 69–288 were identified, which suggests that thrombin cleaves cTnT between R68 and S69.CONCLUSIONSThe results of this study suggest that the 29-kDa fragment of cTnT in AMI serum samples mainly appears due to the cleavage by thrombin during serum sample preparation.

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