Rapid and direct concentration range judgment of lamotrigine in plasma by the multi test lines with different detection limits on the same lateral flow strip

Coronavirus disease 2019 (COVID-19) caused by the SARS-CoV-2 virus has led to a global pandemic with a high spread rate and pathogenicity. Thus, with limited testing solutions, it is imperative to develop early-stage diagnostics for rapid and accurate detection of SARS-CoV-2 to contain the rapid transmission of the ongoing COVID-19 pandemic. In this regard, there remains little knowledge about the integration of the CRISPR collateral cleavage mechanism in the lateral flow assay and fluorophotometer. In the current study, we demonstrate a CRISPR/Cas12a-based collateral cleavage method for COVID-19 diagnosis using the Cas12a/crRNA complex for target recognition, reverse transcription loop-mediated isothermal amplification (RT-LAMP) for sensitivity enhancement, and a novel DNA capture probe-based lateral flow strip (LFS) or real-time fluorescence detector as the parallel system readout facility, termed CRICOLAP. Our novel approach uses a customized reporter that hybridizes an optimized complementary capture probe fixed at the test line for naked-eye result readout. The CRICOLAP system achieved ultra-sensitivity of 1 copy/µL in ~32 min by portable real-time fluorescence detection and ~60 min by LFS. Furthermore, CRICOLAP validation using 60 clinical nasopharyngeal samples previously verified with a commercial RT-PCR kit showed 97.5% and 100% sensitivity for S and N genes, respectively, and 100% specificity for both genes of SARS-CoV-2. CRICOLAP advances the CRISPR/Cas12a collateral cleavage result readout in the lateral flow assay and fluorophotometer, and it can be an alternative method for the decentralized field-deployable diagnosis of COVID-19 in remote and limited-resource locations.

Download Full-text

Image processing for the CCD based lateral flow strip detector

Nano Biomedicine and Engineering ◽

10.5101/nbe.v2i4.p214-217 ◽

2010 ◽

Vol 2 (4) ◽

Cited By ~ 1

Author(s):

Xueqing Zhang ◽

Hao Yang ◽

Kan Wang

Keyword(s):

Image Processing ◽

Lateral Flow ◽

Lateral Flow Strip ◽

Strip Detector

Download Full-text

Rapid and simultaneous visual screening of SARS-CoV-2 and influenza viruses with customized isothermal amplification integrated lateral flow strip

Biosensors and Bioelectronics ◽

10.1016/j.bios.2021.113771 ◽

2021 ◽

pp. 113771

Author(s):

Yong Sun ◽

Panzhu Qin ◽

Jun He ◽

Weiwei Li ◽

Yonglin Shi ◽

...

Keyword(s):

Lateral Flow ◽

Influenza Viruses ◽

Isothermal Amplification ◽

Lateral Flow Strip ◽

Visual Screening

Download Full-text

Development and Performance verification of colloidal gold labeled SARS-CoV-2 antigen detection method for routine popular screening of COVID-19 with clinical samples in Poland and China

10.1101/2021.11.22.21266719 ◽

2021 ◽

Author(s):

Chuanxiang Guo ◽

Li Yao ◽

Fengling Chen ◽

Chao Zhang ◽

Wei Chen

Keyword(s):

Predictive Value ◽

Colloidal Gold ◽

Detection Method ◽

Age Distribution ◽

Antigen Detection ◽

Lateral Flow ◽

Rapid Screening ◽

Clinical Samples ◽

Lateral Flow Strip ◽

And Performance

In this research, we have constructed and optimized the colloidal gold labeled lateral flow strip (LFS) for rapid detection of antigen of SARS-CoV-2 and rapid screening of COVID-19. Based on the constructed and optimized colloidal gold lateral flow strip, the parameters of the LFS have been well evaluated with the clinical samples in the professional labs. The screening performance have also been evaluated from the aspects including the CT values, age distribution and onset of symptoms. Finally, based on the detection results of 420 clinical samples, the LFS can achieve the screening of COVID-19 with the positive percentage agreement (PPA, sensitivity), negative percent agreement (NPA, specificity), the positive predictive value (PPV) and the negative predictive value (NPV) of 96.8%, 100%, 100% and 96.6%, respectively, indicating the powerful potential for practical screening applications in pandemic control. Of great significance, this developed SARS-CoV-2 antigen detection method has also been successfully utilized for screening of delta-variant of SARS-CoV-2.

Download Full-text