Self-setting bioactive calcium–magnesium phosphate cement with high strength and degradability for bone regeneration

Abstract The seek of bioactive materials for promoting bone regeneration is a challenging and long-term task. Functionalization with inorganic metal ions or drug molecules are considered effective strategies to improve the bioactivity of various existing biomaterials. Herein, amorphous calcium magnesium phosphate (ACMP) nanoparticles and simvastatin (SIM)-loaded ACMP (ACMP/SIM) nanocomposites were developed via a simple coprecipitation strategy. The physiochemical property of ACMP/SIM were explored using transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), powder X-ray diffraction (XRD) and high performance liquid chromatograph (HPLC), and the role of Mg2+ in the formation of ACMP/SIM was revealed using X-ray absorption near-edge structure (XANES). After that, the transformation process of ACMP/SIM in simulated body fluid (SBF) was also tracked to simulate and explore the in vivo mineralization performance of materials. We find that ACMP/SIM releases ions of Ca2+, Mg2+ and PO43-, when it is immersed in SBF at 37 °C, and a phase transformation occured during which the initially amorphous ACMP turns into self-assembled hydroxyapatite (HAP). Furthermore, ACMP/SIM displays high cytocompatibility and promotes the proliferation and osteogenic differentiation of MC3T3-E1 cells. For the in vivo studies, lamellar ACMP/SIM/Collagen scaffolds with aligned pore structures were prepared and used to repair a rat defect model in calvaria. ACMP/SIM/Collagen scaffolds show a positive effect in promoting the regeneration of calvaria defect after 12 weeks. The bioactive ACMP/SIM nanocomposites are promising as bone repair materials. Considering the facile preparation process and superior in vitro/vivo bioactivity, the as-prepared ACMP/SIM would be a potential candidate for bone related biomedical applications.

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Injectable bioactive calcium–magnesium phosphate cement for bone regeneration

Biomedical Materials ◽

10.1088/1748-6041/3/4/044105 ◽

2008 ◽

Vol 3 (4) ◽

pp. 044105 ◽

Cited By ~ 39

Author(s):

Fan Wu ◽

Jiacan Su ◽

Jie Wei ◽

Han Guo ◽

Changsheng Liu

Keyword(s):

Bone Regeneration ◽

Magnesium Phosphate ◽

Calcium Magnesium

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Preparation and study of thermostable composites based on solid magnesium phosphate and calcium phosphate binders

Journal of the Belarusian State University Chemistry ◽

10.33581/2520-257x-2021-2-50-61 ◽

2021 ◽

pp. 50-61

Author(s):

Natalia S. Apanasevich ◽

Konstantin N. Lapko ◽

Alexander N. Kudlash ◽

Aliaksei A. Sokal ◽

Yury D. Kliaulin ◽

...

Keyword(s):

Composite Materials ◽

Calcium Phosphate ◽

Thermal Effects ◽

High Strength ◽

Strength Properties ◽

Magnesium Phosphate ◽

Phosphate Binders ◽

Temperature Range ◽

Low Weight ◽

Calcium Magnesium

Thermostable composite materials based on solid magnesium phosphate and calcium phosphate, as well as hybrid calcium magnesium phosphate binders have been developed and investigated. Thermal and phase transformations of the phosphate composites have been studied. Strength characteristics of composite materials have been determined in the temperature range of 20–1000 °C. It is shown that the obtained phosphate composites have high strength properties (compressive strength reaches 120–130 MPa) and are characterised by high thermal stability in the temperature range up to 1000 °С. The low weight loss of the studied composites (no more than 10 %) and the absence of significant thermal effects indicate that they are promising for use as a thermostable matrix for obtaining functional composite materials.

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Multiple Biological Effects of Citrate in Magnesium Phosphate - Based Cement During Bone Regeneration

SSRN Electronic Journal ◽

10.2139/ssrn.3588523 ◽

2020 ◽

Author(s):

Xiaopei Wu ◽

Honglian Dai ◽

Suchun Yu ◽

Yanan Zhao ◽

Yanpiao Long ◽

...

Keyword(s):

Bone Regeneration ◽

Biological Effects ◽

Magnesium Phosphate

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In-Vivo Degradation Behavior and Osseointegration of 3D Powder-Printed Calcium Magnesium Phosphate Cement Scaffolds

Materials ◽

10.3390/ma14040946 ◽

2021 ◽

Vol 14 (4) ◽

pp. 946

Author(s):

Katharina Kowalewicz ◽

Elke Vorndran ◽

Franziska Feichtner ◽

Anja-Christina Waselau ◽

Manuel Brueckner ◽

...

Keyword(s):

Three Dimensional ◽

Bone Substitutes ◽

Magnesium Phosphate ◽

Degradation Behavior ◽

Micro Computed Tomography ◽

Calcium Magnesium ◽

Interest In Research ◽

In Vivo Degradation ◽

Volume Decrease

Calcium magnesium phosphate cements (CMPCs) are promising bone substitutes and experience great interest in research. Therefore, in-vivo degradation behavior, osseointegration and biocompatibility of three-dimensional (3D) powder-printed CMPC scaffolds were investigated in the present study. The materials Mg225 (Ca0.75Mg2.25(PO4)2) and Mg225d (Mg225 treated with diammonium hydrogen phosphate (DAHP)) were implanted as cylindrical scaffolds (h = 5 mm, Ø = 3.8 mm) in both lateral femoral condyles in rabbits and compared with tricalcium phosphate (TCP). Treatment with DAHP results in the precipitation of struvite, thus reducing pore size and overall porosity and increasing pressure stability. Over 6 weeks, the scaffolds were evaluated clinically, radiologically, with Micro-Computed Tomography (µCT) and histological examinations. All scaffolds showed excellent biocompatibility. X-ray and in-vivo µCT examinations showed a volume decrease and increasing osseointegration over time. Structure loss and volume decrease were most evident in Mg225. Histologically, all scaffolds degraded centripetally and were completely traversed by new bone, in which the remaining scaffold material was embedded. While after 6 weeks, Mg225d and TCP were still visible as a network, only individual particles of Mg225 were present. Based on these results, Mg225 and Mg225d appear to be promising bone substitutes for various loading situations that should be investigated further.

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