Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy

Abstract A multi-functionalized graphene oxide (GO)-based carrier with conjugation of aminated-polyethylenglycole (PEG-diamine), octaarginine (R8) and folic acid (FA), which also contains chloroquine (CQ), a lysosomotropic agent, is introduced. The cellular uptake mechanisms and intracellular targeting of FA functionalized nanocarriers are examined. The localized releases of CQ and siRNA intracellular delivery are evaluated. Microencapsulation of the nanocarrier complexed with genes in layer-by-layer coating of alginate micro-beads is also investigated. The covalently co-conjugated FA with PEG and R8 provides a stable formulation with increased cellular uptake compared to FA-free carrier. The CQ-equipped nanocarrier shows a 95% release of CQ at lysosomal pH. The localized release of the drug inside the lysosomes is verified which accelerates the cargo discharge into cytoplasm.

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Bovine α-lactalbumin functionalized graphene oxide nano-sheet exhibits enhanced biocompatibility: A rational strategy for graphene-based targeted cancer therapy

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2015.06.061 ◽

2015 ◽

Vol 134 ◽

pp. 178-187 ◽

Cited By ~ 21

Author(s):

Sailendra Mahanta ◽

Subhankar Paul

Keyword(s):

Graphene Oxide ◽

Cancer Therapy ◽

Targeted Cancer Therapy ◽

Functionalized Graphene ◽

Functionalized Graphene Oxide

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Dual-Functionalized Graphene Oxide Based siRNA Delivery System for Implant Surface Biomodification with Enhanced Osteogenesis

ACS Applied Materials & Interfaces ◽

10.1021/acsami.7b12079 ◽

2017 ◽

Vol 9 (40) ◽

pp. 34722-34735 ◽

Cited By ~ 18

Author(s):

Li Zhang ◽

Qing Zhou ◽

Wen Song ◽

Kaimin Wu ◽

Yumei Zhang ◽

...

Keyword(s):

Graphene Oxide ◽

Delivery System ◽

Sirna Delivery ◽

Functionalized Graphene ◽

Implant Surface ◽

Functionalized Graphene Oxide ◽

Sirna Delivery System

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Functionalized graphene oxide for anti-VEGF siRNA delivery: preparation, characterization and evaluation in vitro and in vivo

RSC Advances ◽

10.1039/c7ra00810d ◽

2017 ◽

Vol 7 (33) ◽

pp. 20553-20566 ◽

Cited By ~ 14

Author(s):

Lulu Ren ◽

Yifan Zhang ◽

Chunying Cui ◽

Yanzhao Bi ◽

Xu Ge

Keyword(s):

Graphene Oxide ◽

Tumor Growth ◽

Tumor Targeting ◽

Sirna Delivery ◽

Functionalized Graphene ◽

Functionalized Graphene Oxide ◽

System P ◽

Targeting Delivery

GO–PLL–SDGR/VEGF-siRNA inhibits tumor growth as a tumor targeting delivery system.

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Tumor microenvironment and NIR laser dual-responsive release of berberine 9-O-pyrazole alkyl derivative loaded in graphene oxide nanosheets for chemo-photothermal synergetic cancer therapy

Journal of Materials Chemistry B ◽

10.1039/d0tb00489h ◽

2020 ◽

Vol 8 (18) ◽

pp. 4046-4055 ◽

Cited By ~ 6

Author(s):

Peifang Du ◽

Jianhua Yan ◽

Shuo Long ◽

Hongjie Xiong ◽

Nachuan Wen ◽

...

Keyword(s):

Graphene Oxide ◽

Tumor Microenvironment ◽

Cancer Therapy ◽

Alkyl Derivative ◽

Functionalized Graphene ◽

Stimuli Responsive ◽

Graphene Oxide Nanosheets ◽

Functionalized Graphene Oxide ◽

Dual Responsive ◽

Nir Laser

Stimuli-responsive release of berberine 9-O-pyrazole alkyl derivative loaded in AS1411-functionalized graphene oxide nanosheets for chemo-photothermal synergetic therapy of cancer.

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