Identification of a chloroplast fatty acid exporter protein, CmFAX1, and triacylglycerol accumulation by its overexpression in the unicellular red alga Cyanidioschyzon merolae

2019 ◽  
Vol 38 ◽  
pp. 101396 ◽  
Author(s):  
Tokiaki Takemura ◽  
Sousuke Imamura ◽  
Kan Tanaka
Keyword(s):  
Fatty Acid ◽  
Red Alga ◽  
Cyanidioschyzon Merolae ◽  
Triacylglycerol Accumulation

scholarly journals Analysis of triacylglycerol accumulation under nitrogen deprivation in the red alga Cyanidioschyzon merolae

Microbiology ◽  
2016 ◽  
Vol 162 (5) ◽  
pp. 803-812 ◽  
Author(s):  
Masakazu Toyoshima ◽  
Natsumi Mori ◽  
Takashi Moriyama ◽  
Osami Misumi ◽  
Naoki Sato
Keyword(s):  
Red Alga ◽  
Cyanidioschyzon Merolae ◽  
Nitrogen Deprivation ◽  
Triacylglycerol Accumulation

Delta-9 fatty acid desaturase gene containing a carboxyl-terminal cytochrome b5 domain from the red alga Cyanidioschyzon merolae

1998 ◽  
Vol 33 (3) ◽  
pp. 165-170 ◽  
Author(s):  
R. Itoh ◽  
Kyoko Toda ◽  
Hidenori Takahashi ◽  
Hiroyoshi Takano ◽  
Tsuneyoshi Kuroiwa
Keyword(s):  
Fatty Acid ◽  
Cytochrome B5 ◽  
Fatty Acid Desaturase ◽  
Red Alga ◽  
Cyanidioschyzon Merolae ◽  
Desaturase Gene ◽  
Carboxyl Terminal ◽  
Fatty Acid Desaturase Gene

scholarly journals Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 971
Author(s):  
Sota Takahashi ◽  
Riho Okubo ◽  
Yu Kanesaki ◽  
Baifeng Zhou ◽  
Kazuhiro Takaya ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Normal Growth ◽  
Growth Conditions ◽  
Red Alga ◽  
Biofuel Production ◽  
Cyanidioschyzon Merolae ◽  
Overexpression Strain ◽  
Liquid Biofuel ◽  
Triacylglycerol Accumulation ◽  
Tag Accumulation

Microalgal triacylglycerols (TAGs) are a good feedstock for liquid biofuel production. Improving the expression and/or function of transcription factors (TFs) involved in TAG accumulation may increase TAG content; however, information on microalgae is still lacking. In this study, 14 TFs in the unicellular red alga Cyanidioschyzon merolae were identified as candidate TFs regulating TAG accumulation using available transcriptome and phosphoproteome data under conditions driving TAG accumulation. To investigate the roles of these TFs, we constructed TF-overexpression strains and analyzed lipid droplet (LD) formation and TAG contents in the cells grown under standard conditions. Based on the results, we identified four TFs involved in LD and TAG accumulation. RNA-Seq analyses were performed to identify genes regulated by the four TFs using each overexpression strain. Among the TAG biosynthesis-related genes, only the gene encoding the endoplasmic reticulum-localized lysophosphatidic acid acyltransferase 1 (LPAT1) was notably increased among the overexpression strains. In the LPAT1 overexpression strain, TAG accumulation was significantly increased compared with the control strain under normal growth conditions. These results indicate that the four TFs positively regulate TAG accumulation by changing their target gene expression in C. merolae.

scholarly journals Occurrence of Cis-11,12-Methylene-Hexadecanoic Acid in the Red Alga Solieria pacifica (Yamada) Yoshida

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2286
Author(s):  
Gwang-Woo Kim ◽  
Jae-Man Sim ◽  
Yutaka Itabashi ◽  
Min-Jeong Jung ◽  
Joon-Young Jun
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Red Alga ◽  
Proton Nuclear Magnetic Resonance ◽  
Animal Origin ◽  
Monoenoic Acid ◽  
Resonance Spectroscopy ◽  
Hexadecenoic Acid ◽  
Cyclopropane Fatty Acid ◽  
Hexadecanoic Acid

Fatty acids in marine algae have attracted the attention of natural chemists because of their biological activity. The fatty acid compositions of the Solieriaceae families (Rhodophyceae, Gaigartinales) provide interesting information that unusual cyclic fatty acids have been occasionally found. A survey was conducted to profile the characteristic fatty acid composition of the red alga Solieria pacifica (Yamada) Yoshida using gas chromatography-mass spectrometry (GC-MS), infrared spectroscopy (IR), and proton nuclear magnetic resonance spectroscopy (1H-NMR). In S. pacifica, two cyclopentyl fatty acids, 11-cyclopentylundecanoic acid (7.0%), and 13-cyclopentyltridecanoic acid (4.9%), and a cyclopropane fatty acid, cis-11,12-methylene-hexadecanoic acid (7.9%) contributed significantly to the overall fatty acid profile. In particular, this cyclopropane fatty acid has been primarily found in bacteria, rumen microorganisms or foods of animal origin, and has not previously been found in any other algae. In addition, this alga contains a significant amount of the monoenoic acid cis-11-hexadecenoic acid (9.0%). Therefore, cis-11,12-methylene-hexadecanoic acid in S. pacifica was likely produced by methylene addition to cis-11-hexadecenoic acid.

scholarly journals A Reaction Center-dependent Photoprotection Mechanism in a Highly Robust Photosystem II from an Extremophilic Red Alga,Cyanidioschyzon merolae

2013 ◽  
Vol 288 (32) ◽  
pp. 23529-23542 ◽  
Author(s):  
Tomasz Krupnik ◽  
Eva Kotabová ◽  
Laura S. van Bezouwen ◽  
Radosław Mazur ◽  
Maciej Garstka ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Reaction Center ◽  
Red Alga ◽  
Cyanidioschyzon Merolae

scholarly journals Effects of milk diets containing beef tallow or coconut oil on the fatty acid metabolism of liver slices from preruminant calves

2000 ◽  
Vol 84 (3) ◽  
pp. 309-318 ◽  
Author(s):  
Benoît Graulet ◽  
Dominique Gruffat-Mouty ◽  
Denys Durand ◽  
Dominique Bauchart
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Oxidation ◽  
Beef Tallow ◽  
Neutral Lipids ◽  
Lipid Production ◽  
Coconut Oil ◽  
Acid Oxidation ◽  
Milk Diet ◽  
Liver Slices ◽  
Triacylglycerol Accumulation

Coconut oil (CO) induces a triacylglycerol infiltration in the hepatocytes of preruminant calves when given as the sole source of fat in the milk diet over a long-term period. Metabolic pathways potentially involved in this hepatic triacylglycerol accumulation were studied by in vitro methods on liver slices from preruminant Holstein × Friesian male calves fed a conventional milk diet containing CO (n 5) or beef tallow (BT, n 5) for 19 d. Liver slices were incubated for 12 h in the presence of 0·8 mM-[14C] oleate or -[14C] laurate added to the medium. Fatty acid oxidation was determined by measuring the production of CO2 (total oxidation) and acid-soluble products (partial oxidation). Production of CO2 was 1·7–3·6-fold lower (P 0·0490) and production of acid-soluble products tended to be lower (P = 0·0625) in liver slices of CO- than BT-fed calves. Fatty acid esterification as neutral lipids was 2·6– to 3·1–fold higher (P = 0·0088) in liver slices prepared from calves fed the CO diet compared with calves fed the BT diet. By contrast with what occurs in the liver of rats fed CO, the increase in neutral lipid production did not stimulate VLDL secretion by the hepatocytes of calves fed with CO, leading to a triacylglycerol accumulation in the cytosol. It could be explained by the reduction of fatty acid oxidation favouring esterification in the form of triacylglycerols, in association with a limited availability of triacylglycerols and/or apolipoprotein B for VLDL packaging and subsequent secretion.

scholarly journals Substrate water exchange in photosystem II core complexes of the extremophilic red alga Cyanidioschyzon merolae

2014 ◽  
Vol 1837 (8) ◽  
pp. 1257-1262 ◽  
Author(s):  
Håkan Nilsson ◽  
Tomasz Krupnik ◽  
Joanna Kargul ◽  
Johannes Messinger
Keyword(s):  
Photosystem Ii ◽  
Water Exchange ◽  
Red Alga ◽  
Cyanidioschyzon Merolae

scholarly journals Detecting Cytoplasmic rRNA in the Primitive Red Alga Cyanidioschyzon merolae.

CYTOLOGIA ◽  
1996 ◽  
Vol 61 (4) ◽  
pp. 439-442 ◽  
Author(s):  
Kyoko Toda ◽  
Hidenori Takahashi ◽  
Ryuuichi Itoh ◽  
Chieko Saito ◽  
Haruko Kuroiwa ◽  
...  
Keyword(s):  
Red Alga ◽  
Cyanidioschyzon Merolae
Sign in / Sign up

Export Citation Format

Share Document