Detection of co-infection of Gardnerella vaginalis and Atopobium vaginae using qualitative PCR: A better predictor of bacterial vaginosis

Anaerobe ◽  
2021 ◽  
Vol 69 ◽  
pp. 102343
Author(s):  
Pragati Grover Sehgal ◽  
Rajneesh Dadwal ◽  
Bhawna Sharma ◽  
Amit Sehgal ◽  
Rashmi Bagga ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Gardnerella Vaginalis ◽  
Qualitative Pcr ◽  
Atopobium Vaginae

scholarly journals Bacterial vaginosis: epidemiologic, clinical and diagnostic updates

2018 ◽  
Vol 32 (4) ◽  
Author(s):  
Giorgio Dirani ◽  
Silvia Zannoli ◽  
Maria Federica Pedna ◽  
Francesco Congestrì ◽  
Patrizia Farabegoli ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Current Status ◽  
Gardnerella Vaginalis ◽  
Vaginal Fluid ◽  
Molecular Approach ◽  
Candida Spp ◽  
Kappa Test ◽  
Lactobacillus Spp ◽  
Standard Techniques ◽  
Atopobium Vaginae

Background and aims. Bacterial vaginosis (BV) is one the more frequently identified genital syndrome among childbearing aged women. The basic condition that generates this condition is a modification in the vaginal microbiota. The aim of this paper is to briefly review the current status of the art of BV and to report the results of a pilot study performed with an innovative PCR based technique. Materials and Methods. 36 samples of vaginal fluid routinely submitted for the diagnosis of BV to the Unit of Microbiology – GRHL were comparatively evaluated by standard techniques and with the HP-Vaginiti e Vaginosi NLM kit that simultaneously detects in a quantitative way specific DNA from Candida (albicans, glabrata; krusei, tropicalis), Gardnerella vaginalis, Lactobacillus spp. and Atopobium vaginae. Results and conclusions. Candida spp. has been identified in 8 samples with culture and in 15 with the molecular test. 29 G. vaginalis were found by PCR whereas only in 7 samples a specific prescription for this microbe was present (of which 4 positive). A. vaginae has been identified in 20 samples by the molecular approach and Lactobacillus spp. was identified in 19 samples (by culture) and in 32 by PCR. The overall diagnosis of BV was made in 9 patients by standard techniques and in 7 by applying the molecular approach. (Cohen’s kappa test: 0,84). The findings of this study clearly demonstrate that the joint use of the routine culture- based techniques with the multiplex PCR methods amplifies by far the sensitivity of the overall diagnostic workflow of BV.

scholarly journals Treatment failure of bacterial vaginosis is not associated with higher loads of Atopobium vaginae and Gardnerella vaginalis

2017 ◽  
Vol 66 (8) ◽  
pp. 1217-1224 ◽  
Author(s):  
Carolina Sanitá Tafner Ferreira ◽  
Gilbert Gerard Donders ◽  
Cristina Maria Garcia de Lima Parada ◽  
Andrea da Rocha Tristão ◽  
Thaiz Fernandes ◽  
...  
Keyword(s):  
Treatment Failure ◽  
Bacterial Vaginosis ◽  
Gardnerella Vaginalis ◽  
Atopobium Vaginae

scholarly journals PECULIARITIES OF DENTAL TREATMENT OF PATIENTS WITH BACTERIAL VAGINOSIS AND THEIR SEX PARTNERS

2020 ◽  
pp. 21-24
Author(s):  
A.D. Krutikova
Keyword(s):  
Oral Cavity ◽  
Bacterial Vaginosis ◽  
Oral Hygiene ◽  
Dental Treatment ◽  
Periodontal Diseases ◽  
Sexual Partners ◽  
Gardnerella Vaginalis ◽  
Initial Degree ◽  
Significant Difference ◽  
Atopobium Vaginae

Patients with bacterial vaginosis are diagnosed with chronic generalized inflammatory and inflammatory-dystrophic periodontal diseases, such as chronic catarrhal gingivitis, periodontitis of initial and first severity. The aim of the study was to compare the effectiveness of the treatment of inflammatory and inflammatory-dystrophic periodontal diseases in women with bacterial vaginosis and their sexual partners. Materials and methods. 28 heterosexual couples aged 18-45 years took part in the survey. Clinical dental examination was performed at the time of treatment and 3 months after completion of treatment. Green-Vermilion indices, RMA (in Parma modification), complex periodontal index according to Leus (KPI), Svrakov's number were determined. Both groups of patients were examined for the presence of Gardnerella vaginalis and Atopobium vaginae in the oral cavity by PCR testing. The therapeutic course was the same for all groups of patients. General therapy included: "Fluconazole" 50 mg 1 time per day (7 days), "Clindamycin" 150 mg every 6 hours (5 days), starting from the 3rd day of antibiotics - probiotic "Symbiter acidophilus concentrated "(21 days), Calcium-D3-Nycomed 1 tab. during dinner 30 days. Local treatment included: oral baths of the drug "Stomatophyte" after morning and evening hygienic procedures (7 days), application of "Metrogil-dent" on the gums (7 days), the drug "Lizak" 1 tab. keep in the mouth until complete dissolution every 6 hours (5 days), after the use of "Stomatophyte" and "Metrogil - dent" - application of "Symbiter omega" on the gums in silicone caps at night (21 days). Results. In women, chronic generalized catarrhal gingivitis was detected in 10.71%, chronic generalized periodontitis of the initial degree in 64.29% and chronic generalized periodontitis of the I degree in 25%. In men who are carriers of pathogens of BV, chronic generalized catarrhal gingivitis was found in 7.14%, chronic generalized periodontitis of the initial degree in 60.71% and chronic generalized periodontitis in grade 32.1. General and local dental treatment of women with bacterial vaginosis and their sexual partners showed a positive objective dynamics of dental status. Thus, after 3 months of clinical and laboratory observations, there were no complaints, the state of oral hygiene improved significantly. If before the treatment the Green-Vermilion index in general in women with BV was 1.323 ± 0.035 points, then after 3 months it was equal to 1.032 ± 0.021. A similar dynamics was determined in men: from the starting index of 1.336 ± 0.041, the Green-Vermilion index decreased to 1.048 ± 0.036. 3 months after treatment, the PMA of patients decreased by 49.03%, in men - by 51.95%, no significant difference between the indicators of groups I and II was registered, while the difference between the results in the middle of groups I and II is significant . According to the KPI index, women had a significant difference between baseline and post-treatment outcomes of 1.98 points, while men had a score of 2.01 points. No significant difference was found between the results of groups I and II both before and after treatment. A similar positive dynamics characterizes the indicator - Srakov's number, according to this indicator before treatment and after differences between groups was not detected. The significant difference between the indicators in the group of women and men was 2,789 and 2,831, respectively. Before treatment and after the detection rate in the oral cavity, Gardnerella vaginalis and Atopobium vaginae did not have a significant difference between the group of men and women, but there was a clear difference between the parameters obtained at the initial examination and 3 months after treatment in the middle and second groups. Thus, Gardnerella vaginalis was detected less by 67.87% and 61.59%, Atopobium vaginae - by 65.79% and 58.65% in women and men, respectively. Conclusions. The developed and patented treatment regimen for patients with bacterial vaginosis contributes to the regression of inflammatory phenomena, improves oral hygiene, and is suitable for the treatment of women with this comorbidity and for the treatment of their sexual partners. The dynamics of reducing the percentage of detection of bacterial vaginosis pathogens corresponded to the dynamics of the clinical picture of periodontal disease in both women with bacterial vaginosis and men who were their sexual partners, which once again confirms the equal effectiveness of treatment for both gender groups.

scholarly journals TACTICS OF DENTAL EXAMINATION OF WOMEN WITH PERIODONTAL DISEASE AGAINST THE BACKGROUND OF BACTERIAL VAGINOSIS AND THEIR SEXUAL PARTNERS

2020 ◽  
Vol 20 (2) ◽  
pp. 206-210
Author(s):  
A.D. Krutikova
Keyword(s):  
Oral Cavity ◽  
Bacterial Vaginosis ◽  
Oral Fluid ◽  
Periodontal Diseases ◽  
Sexual Partners ◽  
Gardnerella Vaginalis ◽  
Dental Examination ◽  
Initial Stage ◽  
Periodontal Index ◽  
Atopobium Vaginae

Numerous studies have proved that periodontal pathology is an issue that requires a comprehensive and multidisciplinary approach in diagnosis, treatment and prevention, as most periodontal diseases are syndromic manifestations of somatic and psycho-somatic diseases, but there are little data on the periodontal status in patients with disturbance of the genital microflora. The aim of the study was to provide the grounds for the tactics of dental examination of child-bearing women with bacterial vaginosis and their sexual partners and to compare the results of different sex groups. Materials and methods. 28 heterosexual couples aged 18-45 years were examined. Objective periodontal examination included the calculation of Green-Vermilion oral hygiene index, papillary-marginal-alveolar index, complex periodontal index by P.A. Leus, Svrakov's iodine number. Amino test of oral fluid, assessment of oral fluid pH, identification of bacterial vaginosis pathogens, and in particular, Gardnerella vaginalis and Atopobium vaginae, in the oral cavity of the patients by polymerase chain reaction-diagnostics and bacterioscopically. Results. Chronic generalized catarrhal gingivitis was detected in 10.71% of the women, chronic generalized periodontitis in the initial stage was found out in 64.29% of the women, and chronic generalized periodontitis of the first degree in 25% of the female patients. Men, who were carriers of bacterial vaginosis, chronic generalized catarrhal gingivitis, made up 7.14%; chronic generalized periodontitis in the initial stage was found in 60.71% of the men and chronic generalized periodontitis was found in 32.14%. The values ​​of periodontal indices did not really differ between the indicators of different sex groups. Amino tests of the oral fluid in the women and men showed a positive result those points out the presence of volatile amines. The oral fluid pH is reduced in patients of both groups that indicate an inflammatory process in the oral cavity. All the patients have "key cells", which are exfoliated epitheliocytes with Atopobium vaginae and Gardnerella vaginalis adhered to them. This is one of the main markers of bacterial vaginosis. Conclusions. Summing up the results of the performed clinical and laboratory observations, it should be noted that when collecting the history of bearing aged women, the dentist should pay attention to the presence of diseases of the genital tract, and in particular, bacterial vaginosis. To detect inflammatory and inflammatory-dystrophic periodontal diseases in women of childbearing age, amino tests of oral fluid not only in women but also in their sexual partners for rapid diagnosis of pathogens such as bacterial vaginosis and vaginal vaginal vagina can be very informative.

scholarly journals In VitroActivity of Nifuratel on Vaginal Bacteria: Could It Be a Good Candidate for the Treatment of Bacterial Vaginosis?

2011 ◽  
Vol 55 (5) ◽  
pp. 2490-2492 ◽  
Author(s):  
Giuseppe Togni ◽  
Valeria Battini ◽  
Anna Bulgheroni ◽  
Federico Mailland ◽  
Maurizio Caserini ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bacterial Vaginosis ◽  
Gardnerella Vaginalis ◽  
Content Type ◽  
Highly Active ◽  
Atopobium Vaginae

ABSTRACTBacterial vaginosis is characterized by a shift of the physiological flora to a diverse spectrum of bacteria, whereGardnerella vaginalisandAtopobium vaginaeare the most important markers. In this study, the antimicrobial activity of nifuratel againstG. vaginalis,A. vaginae, and lactobacilli was compared with that of the two currently used antibiotics metronidazole and clindamycin. Results suggest that nifuratel has a better spectrum of activity, being highly active againstG. vaginalisandA. vaginaewithout affecting lactobacilli.

Identification of Gardnerella vaginalis and Atopobium vaginae in Women With Bacterial Vaginosis in Northern Iran

2019 ◽  
Vol 27 (2) ◽  
pp. 81-84
Author(s):  
Esmaeil Roohbakhsh ◽  
Ali Mojtahedi ◽  
Zahra Roohbakhsh ◽  
Ramezan Ali Khavari-Nejad ◽  
Nour Amirmozafari
Keyword(s):  
Bacterial Vaginosis ◽  
Gardnerella Vaginalis ◽  
Northern Iran ◽  
Atopobium Vaginae

scholarly journals THE CLINICAL AND MICROBIOLOGICAL FEATURES OF BACTERIAL VAGINOSIS

2017 ◽  
pp. 15-18
Author(s):  
Yu. A. Lyzikova
Keyword(s):  
Bacterial Vaginosis ◽  
Sexually Transmitted Infections ◽  
Reproductive Function ◽  
Gardnerella Vaginalis ◽  
Control Group ◽  
Sexually Transmitted ◽  
Female Patients ◽  
Microbiological Examination ◽  
Lactobacillus Spp ◽  
Atopobium Vaginae

Aim : to determine the clinical and microbiological features of bacterial vaginosis on the basis of the microbiological examination, assessment of the cytokine status in female patients. Material and methods . The article presents the results of the complex clinical and microbiological examination of 86 female patients of the fertile age. 30 (34.88 ± 5.14 %) patients were diagnosed bacterial vaginosis on the basis of revelation and identification of DNA of Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp. and the total number of bacteria. The control group consisted of 56 (65.12 ± 5.14 %) patients without bacterial vaginosis. The work also presents the results of the microbiological analysis of the material obtained from the cervical canal and endometrium. All the patients underwent blood tests for detection of the inflammatory reaction - interleukins IL-1, IL-2, tumor necrosis factor (TNF-α), interferon (γ-IFN). Results . The prevalence of bacterial vaginosis among the patients of the reproductive age was 34.8 %. The pathology of the reproductive function was found with equal frequency in the patients of both the groups. Disorders of the immune status in favor of pro-inflammatory cytokines were not diagnosed. The clinical and laboratory criteria made it possible to diagnose bacterial vaginosis in 3.49 % of the patients, the use of PCR diagnosis - in 34.88 %. The concentration of lactobacillus spp. is reliably lower in the patients with bacterial vaginosis, than in the control group (p = 0.0085). As for the concentrations of Gardnerella vaginalis and Atopobium vaginae the groups do not significantly differ. Only 4 (13.33 ± 6.21 %) patients (χ = 5.51, p = 0.02) in the main group detected sexually transmitted infections, which should be taken into account while performing the diagnostic activities. Conclusion. The identification of DNA of certain kinds of microorganisms give an opportunity to assess the state of vaginal microcenosis and the degree of its malfunction even in the absence of clinical and other laboratory signs of bacterial vaginosis. The malfunction of the biocenosis of the genital tract is not associated with disorders of the reproductive function and does not lead to a change in the cytokine status. Patients with bacterial vaginosis are in the risk group for development of sexually transmitted infections, which should be taken into account while performing the diagnostic activities.

scholarly journals CHARACTERISTICS OF THE AMINO TEST AS AN EXPRESS METHOD FOR DETECTING CAUSATIVE AGENTS OF BACTERIAL VAGINOSIS IN ORAL CAVITY

2019 ◽  
Vol 19 (2) ◽  
pp. 164-169
Author(s):  
A.D. Krutikova
Keyword(s):  
Oral Cavity ◽  
Bacterial Vaginosis ◽  
Oral Fluid ◽  
Cross Infection ◽  
Gardnerella Vaginalis ◽  
Express Method ◽  
Group Iii ◽  
Periodontal Tissues ◽  
Pcr Diagnosis ◽  
Atopobium Vaginae

At present, the issue of cross-contamination and cross-infection between the oral cavity and other body sites has not sufficiently studied yet and requires the elaboration of clinical protocols for diagnosis infectious diseases, which the dentists can rely on in their practice. We consider it necessary to highlight the issue of cross-infection between the oral cavity and vagina in women with bacterial vaginosis. This gynaecologic diagnosis draws much attention of healthcare professionals due to its prevalence, the frequency of relapses and the specificity of the microflora causing pathology. The purpose of the work was to characterize the diagnostic value of the amino test of oral fluid as an express method for detecting bacterial vaginosis in the oral cavity. Materials and methods. 106 women of child-bearing age without marked somatic and orthodontic pathologies were examined. Patients were divided into 3 groups depending on their gynaecological status. I group included 25 women with the 1st and 2nd degree of vaginal purity; II group involved 27 individuals – carriers of Gardnerella vaginalis; the third group included 54 women with a confirmed diagnosis of bacterial vaginosis, who were divided into 2 subgroups depending on the prescribed treatment. 26 women who received standard treatment and supportive therapy made up III-A subgroup; 28 patients who received the treatment and preventive therapy aimed at eliminating atypical microflora in the oral cavity and preventing relapse, constituted III-B subgroup. The examination was carried out before starting therapy and in 6 and 12 months following the therapy. The amino-test of oral fluid that we patented is designed for express diagnosis of mixed saliva for the presence of volatile amine of isonitrile produced by bacteria Gardnerella vaginalis and Atopobium vaginae associated with bacterial vaginosis. To confirm the results of the amino test of the oral fluid, a PCR-diagnosis for the presence of Gardnerella vaginalis and Atopobium vaginae in the oral cavity was performed. Results. In women with bacterial vaginosis (group III), chronic catarrhal gingivitis was diagnosed in 12.96%, chronic generalized periodontitis of initial severity was found in 25.93% of the patients studied, chronic generalized periodontitis I severity degree was diagnosed in 61.11% of the patients; clinically intact periodontitis was not registered. Comparing the results of the amino test of oral fluid at all stages of the observation with the results of PCR-diagnosis of Gardnerella vaginalis and Atopobium vaginae, we have found out that the patients of groups I demonstrated complete conformity to the results obtained in the group II: the absence of these microorganisms in the oral cavity was confirmed by negative results of amino test. In the group III-A examined, the percentage of the positive amine test was directly proportional to the changes in the detection of Atopobium vaginae in the oral cavity: before the treatment, this microorganism was detected in 84.6% of the amino test, at the beginning of the survey it was 76.9%; in 6 months following the therapy, the PCR decreased by 26.9%, while the result of the amino test dropped by 34.6%; in a year the detection of Atopobium vaginae increased by 42.3% and the percentage of positive test increased by 34.6%. In groups III-B, direct correlation of the changes in the results of the Gardnerella vaginalis PCR and the amino-test of oral fluid were noted. The primary findings of Gardnerella vaginalis and the positive amine test of women in this group made up 67.9% and 75% respectively. In 6 months, the percentage of Gardnerella vaginalis detection was reduced by 39.3% and by 53.6%, the positive oral test in the oral cavity decreased; in one year, Gardnerella vaginalis was found to be 14.3% less frequent in the patients of group III-B, and the registration of the positive amino test decreased by 7.1%. The obtained data make it possible to suggest the informativeness of the amino test of oral fluid as an express method for detecting bacterial vaginosis agents in the oral cavity, such as Gardnerella vaginalis and Atopobium vaginae, to use this method in the diagnosis of concomitant diseases of periodontal tissues and to prescribe treatment, taking into account the presence of oral microbes from atypical representatives who are resistant to traditional treatment regimens.

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