scholarly journals THE CLINICAL AND MICROBIOLOGICAL FEATURES OF BACTERIAL VAGINOSIS

2017 ◽  
pp. 15-18
Author(s):  
Yu. A. Lyzikova
Keyword(s):  
Bacterial Vaginosis ◽  
Sexually Transmitted Infections ◽  
Reproductive Function ◽  
Gardnerella Vaginalis ◽  
Control Group ◽  
Sexually Transmitted ◽  
Female Patients ◽  
Microbiological Examination ◽  
Lactobacillus Spp ◽  
Atopobium Vaginae

Aim : to determine the clinical and microbiological features of bacterial vaginosis on the basis of the microbiological examination, assessment of the cytokine status in female patients. Material and methods . The article presents the results of the complex clinical and microbiological examination of 86 female patients of the fertile age. 30 (34.88 ± 5.14 %) patients were diagnosed bacterial vaginosis on the basis of revelation and identification of DNA of Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp. and the total number of bacteria. The control group consisted of 56 (65.12 ± 5.14 %) patients without bacterial vaginosis. The work also presents the results of the microbiological analysis of the material obtained from the cervical canal and endometrium. All the patients underwent blood tests for detection of the inflammatory reaction - interleukins IL-1, IL-2, tumor necrosis factor (TNF-α), interferon (γ-IFN). Results . The prevalence of bacterial vaginosis among the patients of the reproductive age was 34.8 %. The pathology of the reproductive function was found with equal frequency in the patients of both the groups. Disorders of the immune status in favor of pro-inflammatory cytokines were not diagnosed. The clinical and laboratory criteria made it possible to diagnose bacterial vaginosis in 3.49 % of the patients, the use of PCR diagnosis - in 34.88 %. The concentration of lactobacillus spp. is reliably lower in the patients with bacterial vaginosis, than in the control group (p = 0.0085). As for the concentrations of Gardnerella vaginalis and Atopobium vaginae the groups do not significantly differ. Only 4 (13.33 ± 6.21 %) patients (χ = 5.51, p = 0.02) in the main group detected sexually transmitted infections, which should be taken into account while performing the diagnostic activities. Conclusion. The identification of DNA of certain kinds of microorganisms give an opportunity to assess the state of vaginal microcenosis and the degree of its malfunction even in the absence of clinical and other laboratory signs of bacterial vaginosis. The malfunction of the biocenosis of the genital tract is not associated with disorders of the reproductive function and does not lead to a change in the cytokine status. Patients with bacterial vaginosis are in the risk group for development of sexually transmitted infections, which should be taken into account while performing the diagnostic activities.

scholarly journals Bacterial vaginosis: epidemiologic, clinical and diagnostic updates

2018 ◽  
Vol 32 (4) ◽  
Author(s):  
Giorgio Dirani ◽  
Silvia Zannoli ◽  
Maria Federica Pedna ◽  
Francesco Congestrì ◽  
Patrizia Farabegoli ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Current Status ◽  
Gardnerella Vaginalis ◽  
Vaginal Fluid ◽  
Molecular Approach ◽  
Candida Spp ◽  
Kappa Test ◽  
Lactobacillus Spp ◽  
Standard Techniques ◽  
Atopobium Vaginae

Background and aims. Bacterial vaginosis (BV) is one the more frequently identified genital syndrome among childbearing aged women. The basic condition that generates this condition is a modification in the vaginal microbiota. The aim of this paper is to briefly review the current status of the art of BV and to report the results of a pilot study performed with an innovative PCR based technique. Materials and Methods. 36 samples of vaginal fluid routinely submitted for the diagnosis of BV to the Unit of Microbiology – GRHL were comparatively evaluated by standard techniques and with the HP-Vaginiti e Vaginosi NLM kit that simultaneously detects in a quantitative way specific DNA from Candida (albicans, glabrata; krusei, tropicalis), Gardnerella vaginalis, Lactobacillus spp. and Atopobium vaginae. Results and conclusions. Candida spp. has been identified in 8 samples with culture and in 15 with the molecular test. 29 G. vaginalis were found by PCR whereas only in 7 samples a specific prescription for this microbe was present (of which 4 positive). A. vaginae has been identified in 20 samples by the molecular approach and Lactobacillus spp. was identified in 19 samples (by culture) and in 32 by PCR. The overall diagnosis of BV was made in 9 patients by standard techniques and in 7 by applying the molecular approach. (Cohen’s kappa test: 0,84). The findings of this study clearly demonstrate that the joint use of the routine culture- based techniques with the multiplex PCR methods amplifies by far the sensitivity of the overall diagnostic workflow of BV.

scholarly journals Quantification of Gardnerella vaginalis, Atopobium vaginae and Lactobacillus spp. in bacterial vaginosis

2021 ◽  
Vol 15 (09) ◽  
pp. 1293-1298
Author(s):  
Nedzib Numanovic ◽  
Snezana Ribis ◽  
Jelena Cukic ◽  
Dane Nenadic ◽  
Aleksandar Zivanovic ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Gardnerella Vaginalis ◽  
Pcr Analysis ◽  
Making A Difference ◽  
The Difference ◽  
Lactobacillus Spp ◽  
Atopobium Vaginae

Introduction: The aim of the study was to investigate prevalence of bacteria most frequently associated with bacterial vaginosis using Amsel’s criteria as well as to quantify these bacteria by real-time PCR and to explore the difference in their quantity between healthy and bacterial vaginosis samples. Methodology: For classification of vaginal discharge samples Amsel’s criteria have been used. To detect and quantify Gardnerella vaginalis Atopobium vaginae, Lactobacillus spp. and total vaginal microbiome, real-time PCR has been applied. Results: According to results of our study Amsel’s criteria matched well with real-time PCR diversification of healthy women and women with BV. Nevertheless, real-time PCR has been more sensitive in diagnosis of bacterial vaginosis. DNA quantification of bacteria demonstrated that mutual abundance of G.vaginalis and A. vaginae was good bacterial vaginosis marker . On the contrary, Lactobacillus spp. was present in high amount in both healthy and bacterial vaginosis samples, but ratio of investigated bacteria was different between them. In fact, G. vaginalis and A. vaginae comprised only 0.1% of total microbiome in healthy, whereas Lactobacillus spp. took 99.3% of it. Nonetheless, in bacterial vaginosis, G. vaginalis and A. vaginae made up 34.4% of total microbiome, while Lactobacillus spp. was 21.6%. Conclusions: According to the results of our study real-time PCR analysis was more sensitive in diagnosis of bacterial vaginosis than Amsel’s method, as well as it represented fine tool in making a difference between microbial entities in healthy and bacterial vaginosis samples.

scholarly journals Association of Female Genital Schistosomiasis with the Cervicovaginal Microbiota and Sexually Transmitted Infections in Zambian Women

2021 ◽  
Author(s):  
Amy S Sturt ◽  
Emily L Webb ◽  
Lisa Himschoot ◽  
Comfort R Phiri ◽  
Joyce Mapani ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Trichomonas Vaginalis ◽  
Mycoplasma Genitalium ◽  
Gardnerella Vaginalis ◽  
Sexually Transmitted ◽  
Weak Evidence ◽  
Key Species ◽  
Atopobium Vaginae ◽  
Female Genital

Abstract Background The cervicovaginal microbiota, including sexually transmitted infections (STI), have not been well-described in female genital schistosomiasis (FGS). Methods Women (aged 18-31, sexually active, non-pregnant) were invited to participate at the final follow-up of HPTN 071 (PopART) Population Cohort in January-August 2018. We measured key species of the cervicovaginal microbiota (Lactobacillus crispatus, L. iners, Gardnerella vaginalis, Atopobium vaginae and Candida) and STI (Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis and Mycoplasma genitalium) using quantitative PCR (qPCR). We evaluated associations of microbiota and STI presence and concentration with FGS (qPCR-detected Schistosoma DNA in any of three genital specimens). Results The presence and concentration of key cervicovaginal species did not differ between participants with (n=30) or without FGS (n=158). A higher proportion of participants with FGS had T. vaginalis compared to FGS negative women (p=0.08), with further analysis showing that T. vaginalis was more prevalent among women with ≥2 Schistosoma qPCR positive genital specimens (50.0%, 8/16) than among FGS negative women (21.5% 34/158, p=0.01). Conclusions We found weak evidence of an association between T. vaginalis presence and FGS, with a stronger association in women with a higher burden FGS infection. Additional research is needed on potential between-parasite interactions, especially regarding HIV-1 vulnerability.

scholarly journals Age and sex trends of Gardnerella vaginalis infection in patients with sexually transmitted infections in Korea

2021 ◽  
Author(s):  
Young Sam Yuk ◽  
Jae Eun Choi ◽  
Jae Kyung Kim
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clinical Laboratory ◽  
Gardnerella Vaginalis ◽  
High Tendency ◽  
Sexually Transmitted ◽  
Female Patients ◽  
Average Patient ◽  
Causative Agents ◽  
Age And Sex

Background and Objectives: Gardnerella vaginalis and Candida albicans are the most common causative agents of bac- terial vaginosis, and infections with these pathogens lead to inflammation, endometritis, and pruritus. The aim of this retro- spective study was to determine the trends of G. vaginalis infections based on real-time PCR data according to age and sex in patients with sexually transmitted diseases. Materials and Methods: A total of 59,381 specimens isolated at a clinical laboratory from September 2018 to December 2020 were subjected to real-time PCR for the detection of G. vaginalis DNA. Sample types included catheter, pus, tissue, swab, and urine samples. Results: Among 59,381 samples, 20,718 (34.8%) were positive for G. vaginalis. Of the positive samples, 13,186 (63.7%) were from male patients and 7,532 (36.3%) were from female patients. Average patient age was 39.1 years (the average age of male and female patients was 38.34 and 40.43 years, respectively). Female patients younger than 19 years exhibited the highest incidence of G. vaginalis, at 71.57%, followed by 68.46% incidence in those aged 20-29 years; the lowest incidence was in women aged 40-49 years. Further, among specimen types, the highest number of G. vaginalis-positive specimens was obtained by the swab sampling method. Conclusion: From 2018 to 2020 in Korea, the number of tests conducted for bacterial vaginosis has increased, while the incidence of G. vaginalis infections appears to have decreased. the finding that female adolescents have a high tendency to carry the pathogen is important. and for effective surveillance of BV, sampling by cotton swabs and detection by multiplex PCR might be a good approach.

Sexually transmitted infections

2021 ◽  
pp. 603-628
Author(s):  
Henrietta Williams
Keyword(s):  
Human Papillomavirus ◽  
Bacterial Vaginosis ◽  
Sexually Transmitted Infections ◽  
Genital Herpes ◽  
Genital Warts ◽  
Syndromic Management ◽  
Sexually Transmitted ◽  
Granuloma Inguinale ◽  
Chlamydial Infections

Why are sexually-transmitted infections important??, Syndromic management of sexually-transmitted infections?, Syphilis?, Gonorrhoea?, Chlamydial infections?, Chancroid?, Granuloma inguinale donovanosis?, Trichomoniasis?, Bacterial vaginosis?, Genital herpes?, Candida vaginitis?, Human papillomavirus and genital warts?

USE OF FEMOFLOR-16 TEST TO ASSESS GENITAL BIOCENOSIS IN WOMEN WITH INFLAMMATORY AND PROLIFERATIVE DISEASES OF CERVIX

2016 ◽  
Vol 1 (61) ◽  
pp. 90-95
Author(s):  
Иевлева ◽  
Nadezhda Ievleva ◽  
Пермина ◽  
Natalya Permina ◽  
Ивахнишина ◽  
...  
Keyword(s):  
Genital Tract ◽  
Inflammatory Diseases ◽  
Female Genital Tract ◽  
Reproductive Age ◽  
Gardnerella Vaginalis ◽  
Control Group ◽  
Healthy Women ◽  
Opportunistic Bacteria ◽  
Obligate Anaerobic ◽  
Atopobium Vaginae

Qualitative and quantitative assessment of microbes making the microbiocenosis of genital tract in women with inflammatory and proliferative diseases of cervix using Femoflor-16 test was the aim of the research. Scrapings of cervix and vaginal fornix in 100 women of reproductive age with cervicitis, vaginitis and in 31 women with cervical pseudoerosion (ectropion) were studied. The control group consisted of 35 relatively healthy women preparing for pregnancy. Cervical and vaginal dysbiosis was found in women with inflammatory diseases of cervix in 37.0% of cases, in women with pseudoerosion in 32.2% of cases. These are 3.3 (р<0.005) and 2.8 (р<0.02) times as much as in the group of healthy women preparing for pregnancy (11.1%). Dysbiosis structure was represented primarily by obligate anaerobic agents such as Gardnerella vaginalis, Atopobium vaginae, Eubacterium spp. in association with other opportunistic bacteria that are clinically most significant microorganisms colonizing female genital tract. Mycoplasma and yeast-like fungi of Candida species were found primarily with anaerobes. Aerobic and mixed dysbiosis were only found in 7% of cases in women with inflammatory diseases. Femoflor-16 test is a readily available, fast, efficient, up-to-date method enabling one to begin with early adequate antibacterial therapy and monitor it.

scholarly journals Treatment failure of bacterial vaginosis is not associated with higher loads of Atopobium vaginae and Gardnerella vaginalis

2017 ◽  
Vol 66 (8) ◽  
pp. 1217-1224 ◽  
Author(s):  
Carolina Sanitá Tafner Ferreira ◽  
Gilbert Gerard Donders ◽  
Cristina Maria Garcia de Lima Parada ◽  
Andrea da Rocha Tristão ◽  
Thaiz Fernandes ◽  
...  
Keyword(s):  
Treatment Failure ◽  
Bacterial Vaginosis ◽  
Gardnerella Vaginalis ◽  
Atopobium Vaginae

scholarly journals PECULIARITIES OF DENTAL TREATMENT OF PATIENTS WITH BACTERIAL VAGINOSIS AND THEIR SEX PARTNERS

2020 ◽  
pp. 21-24
Author(s):  
A.D. Krutikova
Keyword(s):  
Oral Cavity ◽  
Bacterial Vaginosis ◽  
Oral Hygiene ◽  
Dental Treatment ◽  
Periodontal Diseases ◽  
Sexual Partners ◽  
Gardnerella Vaginalis ◽  
Initial Degree ◽  
Significant Difference ◽  
Atopobium Vaginae

Patients with bacterial vaginosis are diagnosed with chronic generalized inflammatory and inflammatory-dystrophic periodontal diseases, such as chronic catarrhal gingivitis, periodontitis of initial and first severity. The aim of the study was to compare the effectiveness of the treatment of inflammatory and inflammatory-dystrophic periodontal diseases in women with bacterial vaginosis and their sexual partners. Materials and methods. 28 heterosexual couples aged 18-45 years took part in the survey. Clinical dental examination was performed at the time of treatment and 3 months after completion of treatment. Green-Vermilion indices, RMA (in Parma modification), complex periodontal index according to Leus (KPI), Svrakov's number were determined. Both groups of patients were examined for the presence of Gardnerella vaginalis and Atopobium vaginae in the oral cavity by PCR testing. The therapeutic course was the same for all groups of patients. General therapy included: "Fluconazole" 50 mg 1 time per day (7 days), "Clindamycin" 150 mg every 6 hours (5 days), starting from the 3rd day of antibiotics - probiotic "Symbiter acidophilus concentrated "(21 days), Calcium-D3-Nycomed 1 tab. during dinner 30 days. Local treatment included: oral baths of the drug "Stomatophyte" after morning and evening hygienic procedures (7 days), application of "Metrogil-dent" on the gums (7 days), the drug "Lizak" 1 tab. keep in the mouth until complete dissolution every 6 hours (5 days), after the use of "Stomatophyte" and "Metrogil - dent" - application of "Symbiter omega" on the gums in silicone caps at night (21 days). Results. In women, chronic generalized catarrhal gingivitis was detected in 10.71%, chronic generalized periodontitis of the initial degree in 64.29% and chronic generalized periodontitis of the I degree in 25%. In men who are carriers of pathogens of BV, chronic generalized catarrhal gingivitis was found in 7.14%, chronic generalized periodontitis of the initial degree in 60.71% and chronic generalized periodontitis in grade 32.1. General and local dental treatment of women with bacterial vaginosis and their sexual partners showed a positive objective dynamics of dental status. Thus, after 3 months of clinical and laboratory observations, there were no complaints, the state of oral hygiene improved significantly. If before the treatment the Green-Vermilion index in general in women with BV was 1.323 ± 0.035 points, then after 3 months it was equal to 1.032 ± 0.021. A similar dynamics was determined in men: from the starting index of 1.336 ± 0.041, the Green-Vermilion index decreased to 1.048 ± 0.036. 3 months after treatment, the PMA of patients decreased by 49.03%, in men - by 51.95%, no significant difference between the indicators of groups I and II was registered, while the difference between the results in the middle of groups I and II is significant . According to the KPI index, women had a significant difference between baseline and post-treatment outcomes of 1.98 points, while men had a score of 2.01 points. No significant difference was found between the results of groups I and II both before and after treatment. A similar positive dynamics characterizes the indicator - Srakov's number, according to this indicator before treatment and after differences between groups was not detected. The significant difference between the indicators in the group of women and men was 2,789 and 2,831, respectively. Before treatment and after the detection rate in the oral cavity, Gardnerella vaginalis and Atopobium vaginae did not have a significant difference between the group of men and women, but there was a clear difference between the parameters obtained at the initial examination and 3 months after treatment in the middle and second groups. Thus, Gardnerella vaginalis was detected less by 67.87% and 61.59%, Atopobium vaginae - by 65.79% and 58.65% in women and men, respectively. Conclusions. The developed and patented treatment regimen for patients with bacterial vaginosis contributes to the regression of inflammatory phenomena, improves oral hygiene, and is suitable for the treatment of women with this comorbidity and for the treatment of their sexual partners. The dynamics of reducing the percentage of detection of bacterial vaginosis pathogens corresponded to the dynamics of the clinical picture of periodontal disease in both women with bacterial vaginosis and men who were their sexual partners, which once again confirms the equal effectiveness of treatment for both gender groups.

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