scholarly journals Effect of Single Layer Centrifugation Porcicoll (70%, 80% and 90%) or supplementation with reduced glutathione, seminal plasma and bovine serum albumin on frozen-thawed boar sperm

2017 ◽  
Vol 187 ◽  
pp. 167-173 ◽  
Author(s):  
I. Crespo-Félez ◽  
A. Castañeda-Sampedro ◽  
D.I. Sánchez ◽  
E. Fernández-Alegre ◽  
M. Álvarez-Rodríguez ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Seminal Plasma ◽  
Bovine Serum ◽  
Reduced Glutathione ◽  
Single Layer ◽  
Boar Sperm

STUDY OF THE TRANSFORMATION OF NITROSYL IRON COMPLEX WITH N-ETHYLTHIOUREA LIGANDS IN MODEL BIOLOGICAL SYSTEMS

2021 ◽  
Author(s):  
Olesya Viktorovna Pokidova ◽  
◽  
Nina Sergeevna Emel’yanova ◽  
Alexander Vasilievich Kulikov ◽  
Alexander Ivanovich Kotelnikov ◽  
...  
Keyword(s):  
Amino Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Reduced Glutathione ◽  
Decay Product ◽  
Iron Complex ◽  
Amino Acid Residues ◽  
Cationic Complex ◽  
Nitrosyl Iron Complex

The process of transformation of a mononuclear cationic complex with N-ethylthiourea ligands in Tris-HCl buffer, as well as in a reaction mixture with reduced glutathione and bovine serum albumin, has been studied. It was found that in the presence of glutathione, the complex dimer-izes, while its initial ligands are replaced by glutathione. In the presence of albumin, the decay product of the complex is coordinated with amino acid residues (Cys34 and His39) to form a protein-bound complex.

On the Interactions Between Bovine Serum Albumin and Reduced Glutathione in Solution

2021 ◽  
Author(s):  
Maria Bercea ◽  
Ioana-Alexandra Plugariu ◽  
Loredana Elena Nita ◽  
Simona Morariu ◽  
Robert Vasile Gradinaru
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Reduced Glutathione

Effects of Bovine Serum Albumin on Boar Sperm Quality During Liquid Storage at 17°C

2015 ◽  
Vol 50 (2) ◽  
pp. 263-269 ◽  
Author(s):  
X-G Zhang ◽  
G-J Yan ◽  
J-Y Hong ◽  
Z-Z Su ◽  
G-S Yang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Sperm Quality ◽  
Liquid Storage ◽  
Boar Sperm

Effects of α-Linolenic Acid and Bovine Serum Albumin on Frozen-thawed Boar Sperm Quality during Cryopreservation

2016 ◽  
Vol 40 (4) ◽  
pp. 33-37 ◽  
Author(s):  
Won-Hee Lee ◽  
◽  
Yong Hwangbo ◽  
Sang-Hee Lee ◽  
Hee-Tae Cheong ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Linolenic Acid ◽  
Bovine Serum ◽  
Sperm Quality ◽  
Boar Sperm

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

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