Silver nanoparticles induce oxidative stress, apoptosis and impaired steroidogenesis in ovarian granulosa cells of cattle

2021 ◽  
pp. 106908
Author(s):  
Mohammad Reza Tabandeh ◽  
Kosar Abbasi Samie ◽  
Erfan Sadeghi Mobarakeh ◽  
Mohammad Darvish Khadem ◽  
Sadegh Jozaie
Keyword(s):  
Oxidative Stress ◽  
Silver Nanoparticles ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells

scholarly journals Protective Effect of L-carnitine on the Oxidative Stress Injury of Human Ovarian Granulosa Cells

2021 ◽  
Author(s):  
Xuening Li ◽  
Xiaodong Wu ◽  
Yuemin Zhang ◽  
Tianyi Ma ◽  
Pingping Sun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Cell Viability ◽  
Granulosa Cells ◽  
Blank Group ◽  
Stress Injury ◽  
Ovarian Granulosa Cells ◽  
Nuclear Pyknosis ◽  
Mda Content

Abstract The protective effect of L-carnitine (LC) on the oxidative stress (OS) injury and the effect of L-carnitine on follicular stimulating hormone receptor (FSHR) of ovarian granulosa cells (GCs) were investigated. OS was induced by treatment with H2O2. We cultured KGN cells in four groups: the blank group, OS group and two L-carnitine pretreatment group (low, high). In the OS group, cell nuclear pyknosis was observed, mitochondria swelled irregularly and their cristae were fractured. Meanwhile, the cell viability, superoxide dismutase (SOD) and glutathione (GSH) contents, mitochondrial membrane potential (ΔΨm) and the level of FSHR expression were significantly decreased in the OS group. However, malonaldehyde (MDA) content, reactive oxygen species (ROS) level and apoptosis rate were significantly increased. Compared with the OS group, the morphology of cells and mitochondria in the L-carnitine pretreatment group were improved, the cell viability and the expression of FSHR was significantly increased, and the OS level was decreased. These results indicated that L-carnitine can protect the cells from OS damage induced by H2O2, enhance the antioxidant and anti-apoptotic ability of GCs, and alleviate the decrease of FSHR expression on GCs caused by OS. Therefore, L-carnitine may help prevent the ovarian aging and improve the quality of follicles.

scholarly journals SOD2 deficiency-induced oxidative stress attenuates steroidogenesis in mouse ovarian granulosa cells

2021 ◽  
Vol 519 ◽  
pp. 110888
Author(s):  
Syed Kashif Zaidi ◽  
Wen-Jun Shen ◽  
Yuan Cortez ◽  
Stefanie Bittner ◽  
Alex Bittner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells

scholarly journals Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells

2019 ◽  
Vol 8 (3) ◽  
pp. 381-394 ◽  
Author(s):  
Anima Tripathi ◽  
Vivek Pandey ◽  
Alakh N. Sahu ◽  
Alok Singh ◽  
Pawan K. Dubey
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells ◽  
Ethylhexyl Phthalate

Increased oxidative stress (OS) due to ubiquitous exposure to di-(2-ethylhexyl) phthalate (DEHP) can affect the quality of oocytes by inducing apoptosis and hampering granulosa cell mediated steroidogenesis.

PKCδ contributes to oxidative stress-induced apoptosis in porcine ovarian granulosa cells via activating JNK

2019 ◽  
Vol 131 ◽  
pp. 89-95 ◽  
Author(s):  
Shuo Liu ◽  
Ming Shen ◽  
Chengyu Li ◽  
Yinghui Wei ◽  
Xueqing Meng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells ◽  
Induced Apoptosis

Genistein protects ovarian granulosa cells from oxidative stress via cAMP‐PKA signaling

2019 ◽  
Vol 44 (2) ◽  
pp. 433-445
Author(s):  
Man Luo ◽  
Zhan‐Qing Yang ◽  
Ji‐Cheng Huang ◽  
Yu‐Si Wang ◽  
Bin Guo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells

Morroniside suppresses hydrogen peroxide-stimulated autophagy and apoptosis in rat ovarian granulosa cells through the PI3K/AKT/mTOR pathway

2020 ◽  
pp. 096032712096076
Author(s):  
D Deng ◽  
J Yan ◽  
Y Wu ◽  
K Wu ◽  
W Li
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Cell Survival ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Cell Apoptosis ◽  
Critical Role ◽  
Mtor Pathway ◽  
Dependent Manner ◽  
Ovarian Granulosa Cells

Previous evidences have indicated that granulosa cells play a critical role in follicular growth. Hydrogen peroxide (H2O2)-induced oxidative stress has been associated with ovarian granulosa cell apoptosis and ovarian function. Recently, a study highlighted the protective role of morroniside against H2O2-induced damage. In this study, we aimed to investigate the effects of morroniside on H2O2-stimulated rat ovarian granulosa cells and its underlying molecular mechanisms. Our results showed that H2O2 treatment suppressed cell survival and increased apoptosis in rat granulosa cells, while treatment with morroniside markedly increased H2O2-induced granulosa cell survival in a dose-dependent manner (0, 10, 50 and 100 µM). Moreover, treatment with 50 µM morroniside impeded H2O2-induced cell apoptosis. An elevation in intracellular ROS, MDA, SOD, GSH-Px, and CAT level was observed in H2O2-induced granulosa cells; however, this effect was abrogated by morroniside treatment. Further studies suggested that administration of morroniside inhibited H2O2-induced granulosa cell apoptosis and caspase-3 activity. In addition, after morroniside treatment of H2O2-stimulated granulosa cells, autophagy-related protein (LC3-II/LC3-I ratio) and beclin-1 expression was decreased and p62 level was increased. Interestingly, we found that morroniside treatment activated the PI3K/AKT/mTOR pathway in H2O2-stimulated granulosa cells. Finally, we showed that treatment with PI3K and mTOR inhibitors reversed the protective effects of morroniside on H2O2-induced granulosa cells. Taken together, our data suggest that treatment with morroniside decreased apoptosis, autophagy, and oxidative stress in rat granulosa cells through the PI3K/AKT/mTOR pathway.

scholarly journals Effects of Multicycle Gonadotropin-releasing Hormone Antagonist Protocols on Oxidative Stress of Follicular Fluid and Ovarian Granulosa Cells

2020 ◽  
Author(s):  
Yucong Ma ◽  
Zhiming Zhao ◽  
Guimin Hao ◽  
Na Cui ◽  
Yanli Fan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Gonadotropin Releasing Hormone ◽  
Clinical Pregnancy ◽  
Vitro Fertilization ◽  
Releasing Hormone ◽  
Ovarian Granulosa Cells ◽  
Cycle Group

Abstract Background: Repeated controlled ovarian stimulation (COS) has adverse effects on clinical pregnancy outcomes in in vitro fertilization-embryo transfer (IVF-ET) patients. The effect of repeated antagonist protocols on oxidative stress (OS) in follicular fluid (FF) and ovarian granulosa cells (GC) remains unclear. Objective: To study the effects of repeated multicycle gonadotropin-releasing hormone antagonist (GnRH-ant) protocols on OS markers of FF and ovarian GC. Methods: A total of 145 patients were enrolled and divided into four groups: 1 cycle group (n = 42), 2 cycles group (n = 37), 3 cycles group (n = 45), and 4-5 cycles group (n = 21). The FF and ovarian GC of the patients were collected on the day of last egg retrieval. Levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in FF and ovarian GC were analyzed. Results: With the increase of GnRH-ant protocol cycles, the serum estradiol levels on human chorionic gonadotrophin (hCG) injection day, number of retrieved oocytes, 2PN embryos, and the rates of high-quality embryos, implantation and clinical pregnancy were all significantly decreased, while the gonadotropin (Gn) usage showed an increasing trend. Compared with 1 or 2 cycles, the 8-OHdG and SOD were significantly increased in the 3 to 5 cycles, while the CAT and GSH-Px levels were significantly decreased. Conclusion: Repeated COS with the use of GnRH-ant protocols results in OS and changes the follicle microenvironment of FF and GC, possibly leading to poor IVF outcomes in patients with 3-5 cycles of COS.

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