scholarly journals P-248 Determination of the optimal electrical field for apoptosis in the rat stomach during irreversible electroporation

2021 ◽  
Vol 32 ◽  
pp. S183
Author(s):  
G. Park ◽  
H. Chun ◽  
H. Jeon ◽  
H. Choi ◽  
E. Kim ◽  
...  
Keyword(s):  
Irreversible Electroporation ◽  
Rat Stomach ◽  
Electrical Field

scholarly journals ELECTRICAL CONTROL OF RHIZOID FORMATION IN THE RED ALGA, GRIFFITHSIA BORNETIANA

1934 ◽  
Vol 18 (1) ◽  
pp. 1-21 ◽  
Author(s):  
Victor Schechter
Keyword(s):  
Red Alga ◽  
Ph Change ◽  
Electrical Field ◽  
Galvanic Current ◽  
Electrical Control ◽  
Differentiated Cells ◽  
Cytoplasmic Bodies ◽  
Bright Pink ◽  
Rhizoid Formation

1. Direct galvanic current of 10 to 40 microamperes per square millimeter of cross-section of medium results in anodal determination of rhizoid origin in the differentiated cells of the red alga Griffithsia bornetiana. The current is most effective near the upper end of the range. 2. Within the range used there is an increase in the number of rhizoids produced with increase in current intensity and a decrease in size of rhizoids. 3. Currents of lower intensity require a longer time to produce these effects than comparatively high currents. 4. The orientation of the plants in the electrical field seems to affect the number of rhizoids produced, in that plants with apexes toward the anode produce more rhizoids. 5. Together with anodal rhizoid determination there is migration of chromatophores toward the anodal side of each cell. 6. Displacement of chromatophores (and other cytoplasmic bodies) by the centrifuge does not affect the point of rhizoid origin, but does affect the shoots. 7. Together with anodal determination of rhizoids the algal filaments become graded in color, from bright pink toward the cathode to pale tan toward the anode. 8. Evidence is presented to show that this is not due to a pH change, but to a loss of pigment by chromatophores toward the anode and electrophoresis of the pigment toward the cathode. 9. In conclusion the probability is pointed out that the current acts in morphogenesis by moving particles of different charge.

scholarly journals Numerical simulation modeling of the irreversible electroporation treatment zone for focal therapy of prostate cancer, correlation with whole-mount pathology and T2-weighted MRI sequences

2019 ◽  
Vol 11 ◽  
pp. 175628721985230 ◽  
Author(s):  
Matthijs J. Scheltema ◽  
Tim J. O’Brien ◽  
Willemien van den Bos ◽  
Daniel M. de Bruin ◽  
Rafael V. Davalos ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Irreversible Electroporation ◽  
Prostate Tissue ◽  
Human Prostate ◽  
Ablation Zone ◽  
Electrical Field ◽  
Whole Mount ◽  
Human Prostate Tissue ◽  
Mri Sequences

Background: At present, it is not possible to predict the ablation zone volume following irreversible electroporation (IRE) for prostate cancer (PCa). This study aimed to determine the necessary electrical field threshold to ablate human prostate tissue in vivo with IRE. Methods: In this prospective multicenter trial, patients with localized PCa were treated with IRE 4 weeks before their scheduled radical prostatectomy. In 13 patients, numerical models of the electrical field were generated and compared with the ablation zone volume on whole-mount pathology and T2-weighted magnetic resonance imaging (MRI) sequences. Volume-generating software was used to calculate the ablation zone volumes on histology and MRI. The electric field threshold to ablate prostate tissue was determined for each patient. Results: A total of 13 patients were included for histological and simulation analysis. The median electrical field threshold was 550 V/cm (interquartile range 383–750 V/cm) for the software-generated histology volumes. The median electrical field threshold was 500 V/cm (interquartile range 386–580 V/cm) when the ablation zone volumes were used from the follow-up MRI. Conclusions: The electrical field threshold to ablate human prostate tissue in vivo was determined using whole-mount pathology and MRI. These thresholds may be used to develop treatment planning or monitoring software for IRE prostate ablation; however, further optimization of simulation methods are required to decrease the variance that was observed between patients.

Anionic and Cationic Diffusion in Ionic Conducting Oxides

2005 ◽  
Vol 237-240 ◽  
pp. 843-848 ◽  
Author(s):  
Georgette Petot-Ervas ◽  
C. Petot ◽  
Jean Marc Raulot ◽  
J. Kusinski
Keyword(s):  
Chemical Potential ◽  
Potential Gradient ◽  
Transport Processes ◽  
Electrical Field ◽  
Experimental Procedure ◽  
Conducting Oxides ◽  
Self Diffusion ◽  
Ionic Conducting ◽  
Applied Electrical Field

This paper concerns an analysis of the transport processes at high temperature in anionic conducting oxides subjected to a chemical potential gradient or an applied electrical field. The general equations are given. The principle of the cationic kinetic demixing under a “generalized“ thermodynamical potential gradient is reviewed. Experimental results obtained with yttria-doped zirconia are reported. An experimental procedure for the determination of the oxygen diffusion coefficient in ionic and semiconducting oxides is also described. The results obtained with yttriastabilized zirconia are compared to both self diffusion and conductivity data. This has allowed us to obtain information concerning the defect structure.

scholarly journals Reversible and irreversible electroporation of cell suspensions flowing through a localized DC electric field

2013 ◽  
Vol 18 (1) ◽  
Author(s):  
Włodzimierz Korohoda ◽  
Maciej Grys ◽  
Zbigniew Madeja
Keyword(s):  
Fluorescent Dyes ◽  
Irreversible Electroporation ◽  
Cell Suspensions ◽  
Field Exposure ◽  
Prostate Cell ◽  
Cell Electroporation ◽  
Human Red Blood Cells ◽  
Dc Electric Field

AbstractExperiments on reversible and irreversible cell electroporation were carried out with an experimental setup based on a standard apparatus for horizontal electrophoresis, a syringe pump with regulated cell suspension flow velocity and a dcEF power supply. Cells in suspension flowing through an orifice in a barrier inserted into the electrophoresis apparatus were exposed to defined localized dcEFs in the range of 0–1000 V/cm for a selected duration in the range 10–1000 ms. This method permitted the determination of the viability of irreversibly electroperforated cells. It also showed that the uptake by reversibly electroperforated cells of fluorescent dyes (calcein, carboxyfluorescein, Alexa Fluor 488 Phalloidin), which otherwise do not penetrate cell membranes, was dependent upon the dcEF strength and duration in any given single electrical field exposure. The method yields reproducible results, makes it easy to load large volumes of cell suspensions with membrane non-penetrating substances, and permits the elimination of irreversibly electroporated cells of diameter greater than desired. The results concur with and elaborate on those in earlier reports on cell electroporation in commercially available electroporators. They proved once more that the observed cell perforation does not depend upon the thermal effects of the electric current upon cells. In addition, the method eliminates many of the limitations of commercial electroporators and disposable electroporation chambers. It permits the optimization of conditions in which reversible and irreversible electroporation are separated. Over 90% of reversibly electroporated cells remain viable after one short (less than 400 ms) exposure to the localized dcEF. Experiments were conducted with the AT-2 cancer prostate cell line, human skin fibroblasts and human red blood cells, but they could be run with suspensions of any cell type. It is postulated that the described method could be useful for many purposes in biotechnology and biomedicine and could help optimize conditions for in vivo use of both reversible and irreversible electroporation.

scholarly journals The Effects of Feeding Obese Rats by Bee Bread on IL-6 Expression in Rat Stomach

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1570
Author(s):  
Zuleyha Doganyigit ◽  
Birkan Yakan ◽  
Emin Kaymak
Keyword(s):  
Interleukin 6 ◽  
High Fat Diet ◽  
High Fat ◽  
Rat Stomach ◽  
Bee Bread ◽  
Obese Rats

The aim of this project was the determination of the effect of bee bread supplement in diets of obese rats on interleukin 6 immunreactivity. This study has been shown that bee bread against obesity with a high-fat diet reduces IL-6 expression in the stomach.

Sa1417 The Effect of Irreversible Electroporation in Rat Stomach: According to Different Electric Energy

2013 ◽  
Vol 77 (5) ◽  
pp. AB198
Author(s):  
Hyuk Soon Choi ◽  
Eun Sun Kim ◽  
Bora Keum ◽  
Yoon Tae Jeen ◽  
Hong Sik Lee ◽  
...  
Keyword(s):  
Irreversible Electroporation ◽  
Electric Energy ◽  
Rat Stomach

Etude quantitative de l'énergie libre de PbZrO3 pur et dopé avec Nb2O5

1969 ◽  
Vol 47 (22) ◽  
pp. 2439-2443 ◽  
Author(s):  
L. Benguigui ◽  
H. Hervet
Keyword(s):  
Free Energy ◽  
Ferroelectric Phase ◽  
Paraelectric Phase ◽  
Temperature Phase ◽  
Phase Determination ◽  
Electrical Field ◽  
Antiferroelectric Phase ◽  
Field Temperature ◽  
Heat Transition

Pure and Nb2O5 doped PbZrO3 exhibit the following phases: antiferroelectric, ferroelectric, and paraelectric. By means of measurements of heat transition, dielectric constant in paraelectric phase, determination of electrical field – temperature phase diagrams, we calculate the free energy of each phase. Given that the free energy can be developed in powers of the polarization, we show the coefficients of the ferroelectric phase are slightly modified by the addition of Nb2O5, while those of the antiferroelectric phase vary by a factor greater than two.

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