Sa1417 The Effect of Irreversible Electroporation in Rat Stomach: According to Different Electric Energy

2013 ◽  
Vol 77 (5) ◽  
pp. AB198
Author(s):  
Hyuk Soon Choi ◽  
Eun Sun Kim ◽  
Bora Keum ◽  
Yoon Tae Jeen ◽  
Hong Sik Lee ◽  
...  
Keyword(s):  
Irreversible Electroporation ◽  
Electric Energy ◽  
Rat Stomach

scholarly journals P-248 Determination of the optimal electrical field for apoptosis in the rat stomach during irreversible electroporation

2021 ◽  
Vol 32 ◽  
pp. S183
Author(s):  
G. Park ◽  
H. Chun ◽  
H. Jeon ◽  
H. Choi ◽  
E. Kim ◽  
...  
Keyword(s):  
Irreversible Electroporation ◽  
Rat Stomach ◽  
Electrical Field

scholarly journals Histological and Mathematical Analysis of the Irreversibly Electroporated Liver Tissue

2016 ◽  
Vol 16 (4) ◽  
pp. 488-496 ◽  
Author(s):  
Chang Kyu Sung ◽  
Hong Bae Kim ◽  
Jong Hyun Jung ◽  
Ku Youn Baik ◽  
Kee Wook Moon ◽  
...  
Keyword(s):  
Energy Density ◽  
Liver Tissue ◽  
Irreversible Electroporation ◽  
Electric Energy ◽  
Clinical Results ◽  
Density Level ◽  
Sprague Dawley ◽  
Ablation Area ◽  
Mathematical Calculation ◽  
Electric Energy Density

Irreversible electroporation has clinically been used to treat various types of cancer. A plan on how to apply irreversible electroporation before practicing is very important to increase the ablation area and reduce the side effects. Several electrical models have been developed to predict the ablation area with applied electric energy. In this experiment, the static relationship between applied electric energy and ablated area was mathematically and experimentally investigated at 10 hours after applying irreversible electroporation. We performed the irreversible electroporation on the liver tissue of Sprague Dawley rats (male, 8 weeks, weighing 250-350 g). The ablated area was measured based on histological analysis and compared with the mathematical calculation from the electric energy, assuming that the tissue is homogeneous. The ablated area increased with the increase in applied electric energy. The numerically calculated contour lines of electric energy density overlapped well with the apoptotic area induced by the irreversible electroporation. The overlapped area clearly showed that the destructive threshold of apoptosis between electrodes is electric energy density level of 5.9 × 105 J/m3. The results of the present study suggested that the clinical results of the irreversible electroporation on a liver tissue could be predicted through mathematical calculation.

Electron-Microscopic localization of biotinylated gastrin bound to eosinophils in the rat stomach

1994 ◽  
Vol 52 ◽  
pp. 300-301
Author(s):  
Caroline A. Miller ◽  
David H. Nichols ◽  
Richard F. Murphy
Keyword(s):  
Electron Microscope ◽  
Phosphate Buffer ◽  
Uranyl Acetate ◽  
List Type ◽  
Cerium Chloride ◽  
Rat Stomach ◽  
Electron Microscopic ◽  
Human Sequence ◽  
Acetate Lead ◽  
Microscope Slides

Gastrin is a small peptide capable of both stimulating gastric acid secretion and acting as an enteric growth factor. Known functions of eosinophils in the rat stomach are related to immunological defense. Here we demonstrate the binding of biotinylated gastrin to rat stomach eosinophils in the electron microscope. Small pieces of stomach were fixed by immersion in 4% paraformaldehyde/0.1% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4 for 1 hour. The tissue was then cryoprotected in 30% sucrose/0.1 M phosphate buffer, transferred to Tissue Tek OCT compound and frozen in isopentane cooled with liquid nitrogen. Transverse cryostat sections were cut at 25 μm, thawed in PBS and free floating sections exposed to 10−5 M biotinylated 1-17 gastrin (human sequence; Peninsula Labs) for 1 hour. Controls omitted the biotinylated gastrin from this step. Sections were then rinsed 3X in PBS and exposed to either:1).a 1:50 dilution of 10 nm Extravidin colloidal gold (Sigma) for 2 hours, or2).an avidin-biotin-alkaline phosphatase complex (ABC-AP;Vector) for 1 hour. A substrate solution containing cerium chloride was used to generate an electron dense reaction product.Sections from both procedures were postfixed in 1% OsO4 in 0.1 M phosphate buffer, rinsed and dehydrated. These were then flat embedded in EMbed 812 between two microscope slides coated with Liquid Release (both from Electron Microscopy Sciences).Polymerized sections were adhered to resin blocks using super glue, cut at 70-90 nm, stained with uranyl acetate/lead citrate and observed in a Philips CM-10 electron microscope.

Sustainable 2,5-furandicarboxylic synthesis by a direct 5-hydroxymethylfurfural fuel cell based on a bifunctional PtNiSx catalyst

2020 ◽  
Vol 56 (88) ◽  
pp. 13611-13614
Author(s):  
Jialu Wang ◽  
Xian Zhang ◽  
Guozhong Wang ◽  
Yunxia Zhang ◽  
Haimin Zhang
Keyword(s):  
Fuel Cell ◽  
Electric Energy ◽  
Value Added ◽  
Chemical Energy ◽  
New Type

A new type of direct 5-hydroxymethylfurfural (HMF) oxidation fuel cell based on a bifunctional PtNiSx/CB catalyst not only transformed chemical energy into electric energy but also converted HMF into value-added 2,5-furandicarboxylic (FDCA).

Effect of Interleukin-8 on histamine release from isolated rat stomach

2001 ◽  
Vol 120 (5) ◽  
pp. A159-A159
Author(s):  
S RO ◽  
K YAKABI ◽  
T NAKAMURA
Keyword(s):  
Histamine Release ◽  
Interleukin 8 ◽  
Rat Stomach ◽  
Isolated Rat

DEVELOPMENT OF NOVEL ENDOSCOPIC IRREVERSIBLE ELECTROPORATION ABLATION DEVICE

2018 ◽  
Author(s):  
G Min ◽  
HS Choi ◽  
W Kim ◽  
SJ Choi ◽  
JM Lee ◽  
...  
Keyword(s):  
Irreversible Electroporation

Thermal/electric energy generation and CO2 production for greenhouse facilities

2019 ◽  
Vol 2 (3) ◽  
pp. 141-151
Author(s):  
O. E. Gnezdova ◽  
E. S. Chugunkova
Keyword(s):  
Carbon Dioxide ◽  
Power Distribution ◽  
Power Plants ◽  
Electric Energy ◽  
Electricity Consumption ◽  
Vegetable Crops ◽  
Carbon Dioxide Injection ◽  
Electricity Rates ◽  
Traditional Patterns ◽  
Generation Power

Introduction: greenhouses need microclimate control systems to grow agricultural crops. The method of carbon dioxide injection, which is currently used by agricultural companies, causes particular problems. Co-generation power plants may boost the greenhouse efficiency, as they are capable of producing electric energy, heat and cold, as well as carbon dioxide designated for greenhouse plants.Methods: the co-authors provide their estimates of the future gas/electricity rates growth in the short term; they have made a breakdown of the costs of greenhouse products, and they have also compiled the diagrams describing electricity consumption in case of traditional and non-traditional patterns of power supply; they also provide a power distribution pattern typical for greenhouse businesses, as well as the structure and the principle of operation of a co-generation unit used by a greenhouse facility.Results and discussion: the co-authors highlight the strengths of co-generation units used by greenhouse facilities. They have also identified the biological features of carbon dioxide generation and consumption, and they have listed the consequences of using carbon dioxide to enrich vegetable crops.Conclusion: the co-authors have formulated the expediency of using co-generation power plants as part of power generation facilities that serve greenhouses.

ПРЕРЫВИСТОЕ ОСВЕЩЕНИЕ ПОВЫШАЕТ ЭФФЕКТИВНОСТЬ ИСКУССТВЕННОГО ОСЕМЕНЕНИЯ КУР В КЛЕТКАХ

2019 ◽  
Keyword(s):  
Reproductive Performance ◽  
Artificial Insemination ◽  
Egg Production ◽  
Laying Hens ◽  
Electric Energy ◽  
Productive Performance ◽  
Feed Conversion ◽  
Scientific Center ◽  
Academy Of Sciences ◽  
Egg Fertility

Intermittent Lighting Improves the Efficiency of Artificial Insemination in Cage Housed Laying Hens Kavtarashvili A.Sh., Kolokolnikova T.N. Federal Scientific Center “All-Russian Research and Technological Poultry Institute” of Russian Academy of Sciences Omsk Agrarian Scientific Center Summary: The effects of different lighting regimes on the oviposition schedule, productive performance, and reproductive efficiency in cage housed laying hens of layer parental flock (Hisex White-R) were studied; the reasonable regime of artificial insemination (AI) under intermittent lighting is proposed. It was found that intermittent lighting regime 1L:4D:4L:1D:4L:10D compared to the constant lighting significantly alters oviposition schedule: under this regime 82.3% of all daily eggs were laid until 9 am (vs. 66.6% in control). This regime and AI at 10 am improved the productive and reproductive performance compared to control (constant lighting 16L:8D and AI at 12 am): mortality by 1.9%, egg production per initial hen by 3.8%, egg weight by 1.1%, percentage of eggs suitable for incubation by 1.9%, egg fertility by 0.9%, hatchability by 2.3%, hatch of chicks by 2.9%, feed conversion ratio (kg of feed per 10 eggs) by 5.3%, the expenses of electric energy for lighting (per 1000 eggs suitable for incubation) by 54.5%. Key words: INTERMITTENT LIGHTING, CAGE HOUSED LAYERS, ARTIFICIAL INSEMINATION (AI), OVIPOSITION SCHEDULE, AI TIMING, PRODUCTIVE PERFORMANCE, REPRODUCTIVE PERFORMANCE

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