Cadaverine reverse transporter (CadA protein) contributes to the virulence of Aeromonas veronii TH0426

Abstract Background Aeromonas veronii is a bacterial pathogen in aquaculture, which produces virulence factors to enable it colonize and evade host immune defense. Given that experimental verification of virulence factors is time-consuming and laborious, few virulence factors have been characterized. Moreover, most studies have only focused on single virulence factors, resulting in biased interpretation of the pathogenesis of A. veronii. Results In this study, a PPI network at genome-wide scale for A. veronii was first constructed followed by prediction and mapping of virulence factors on the network. When topological characteristics were analyzed, the virulence factors had higher degree and betweenness centrality than other proteins in the network. In particular, the virulence factors tended to interact with each other and were enriched in two network modules. One of the modules mainly consisted of histidine kinases, response regulators, diguanylate cyclases and phosphodiesterases, which play important roles in two-component regulatory systems and the synthesis and degradation of cyclic-diGMP. Construction of the interspecies PPI network between A. veronii and its host Oreochromis niloticus revealed that the virulence factors interacted with homologous proteins in the host. Finally, the structures and interacting sites of the virulence factors during interaction with host proteins were predicted. Conclusions The findings here indicate that the virulence factors probably regulate the virulence of A. veronii by involving in signal transduction pathway and manipulate host biological processes by mimicking and binding competitively to host proteins. Our results give more insight into the pathogenesis of A. veronii and provides important information for designing targeted antibacterial drugs.

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Enhancement of the production of L-glutaminase, an anticancer enzyme, from Aeromonas veronii by adaptive and induced mutation techniques

PLoS ONE ◽

10.1371/journal.pone.0181745 ◽

2017 ◽

Vol 12 (8) ◽

pp. e0181745 ◽

Cited By ~ 7

Author(s):

S. Aravinth Vijay Jesuraj ◽

Md. Moklesur Rahman Sarker ◽

Long Chiau Ming ◽

S. Marylin Jeya Praya ◽

M. Ravikumar ◽

...

Keyword(s):

Induced Mutation ◽

Aeromonas Veronii

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Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv. sobria Strain K133 which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms

International Journal of Molecular Sciences ◽

10.3390/ijms22084272 ◽

2021 ◽

Vol 22 (8) ◽

pp. 4272

Author(s):

Katarzyna Dworaczek ◽

Maria Kurzylewska ◽

Magdalena Laban ◽

Dominika Drzewiecka ◽

Agnieszka Pękala-Safińska ◽

...

Keyword(s):

Epidemiological Data ◽

13C Nmr Spectroscopy ◽

Freshwater Species ◽

Fish Farms ◽

1H And 13C Nmr ◽

Aeromonas Veronii ◽

Specific Polysaccharide ◽

O Antigens ◽

Unique Composition

In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv. sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm. The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy. It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup. Western blotting and ELISA confirmed that A. veronii bv. sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp. in Polish aquacultures. Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.

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