Apparent dry matter, energy, protein and amino acid digestibility of four soybean ingredients in white shrimp Litopenaeus vannamei juveniles

ABSTRACTThis study was conducted to evaluate the digestibility of biofloc meal collected from catfish culture as a feed raw material for Pacific white shrimp Litopenaeus vannamei diet. A basal feed with 43% crude protein content was used as a control diet and mixed with 2% of binders and 0.5 % of Cr2O3 as a marker for digestibility. The experimental diets were made by mixing 67.5% of the basal diet with 30% of biofloc meal, 2% of binders and 0.5 % of Cr2O3. Nine units of glass tanks (90 cm ×40 cm× 35 cm) filled with 100 L seawater were used as the experimental culture units. White shrimp with an average body weight of 5.61 ± 0.09 g was randomly distributed to each experimental tank at a density of 20 shrimp/tank. The feed was offered at a level of 5% shrimp biomass per day at a frequency of four times a day. The results showed that the dry matter digestibility of feed with 30% biofloc meal in shrimp were similar to that of the reference diet. However, protein and fat digestibility of feed containing biofloc meal were considerably higher than those of the reference diet. Feeding shrimp with 30% biofloc meal diet resulted in higher survival and specific growth rate and lower feed conversion ratio than those of the control. The digestibility of bioflocs dry matter, protein and lipid in Pacific white shrimp obtained in this study were 54.9%, 76.3% and 79.3%, respectively.Keywords: biofloc, digestibility, catfish, shrimp ABSTRAKPenelitian ini dilakukan untuk mengevaluasi kecernaan tepung bioflok yang diambil dari media pemeliharaan ikan lele sebagai bahan pakan udang vaname Litopenaeus vannamei. Pakan yang mengandung kadar protein 43% digunakan sebagai pakan control dan dicampur dengan 2% binder dan 0.5% Cr2O3 sebagai marker untuk kecernaan. Pakan perlakuan dibuat dengan mencampurkan 67.5% pakan control dengan 30% tepung bioflok, 2% binder dan 0.5% Cr2O3. Penelitian menggunakan sembilan unit akuarium (90 cm ×40 cm ×35 cm) yang diisi 100 L air laut. Udang vaname dengan bobot rata-rata 5.61 ± 0.09 g ditebar secara acak pada setiap akuarium perlakuan pada kepadatan 20 ekor/akuarium. Pakan diberikan dengan tingkat pemberian pakan 5% biomassa per hari sebanyak empat kali sehari. Hasil penelitian ini menunjukkan bahwa kecernaan pakan dengan 30% tepung bioflok tidak berbeda nyata dengan pakan acuan. Namun kecernaan protein dan lemak pakan yang mengandung tepung bioflok terlihat lebih tinggi daripada pakan kontrol. Pemberian pakan dengan tepung bioflok sebanyak 30% juga menghasilkan tingkat kelangsungan hidup, laju pertumbuhan spesifik udang dan rasio konversi pakan yang lebih baik daripada udang yang diberi pakan kontrol. Kecernaan bahan, protein dan lemak tepung bioflok pada udang yang didapat dalam penelitian masing-masing adalah 54.9%, 76.3% dan 79.3%.Kata-kata kunci: bioflok, ikan lele, kecernaan, udang

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Nitrogen isotope fractionation and amino acid turnover rates in the Pacific white shrimp Litopenaeus vannamei

Marine Ecology Progress Series ◽

10.3354/meps11030 ◽

2014 ◽

Vol 516 ◽

pp. 239-250 ◽

Cited By ~ 13

Author(s):

EE Downs ◽

BN Popp ◽

CM Holl

Keyword(s):

Amino Acid ◽

Litopenaeus Vannamei ◽

Isotope Fractionation ◽

Nitrogen Isotope ◽

Pacific White Shrimp ◽

White Shrimp ◽

Turnover Rates ◽

Nitrogen Isotope Fractionation ◽

The Pacific ◽

Amino Acid Turnover

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Dark and Yellow Rapeseed Hulls, Soybean Hulls and a Purified Fiber Source: Their Effects on Dry Matter, Energy, Protein and Amino Acid Digestibilities in Cannulated Pigs

Journal of Animal Science ◽

10.2527/jas1984.5961510x ◽

1984 ◽

Vol 59 (6) ◽

pp. 1510-1518 ◽

Cited By ~ 24

Author(s):

B. N. Mitaru ◽

R. Blair ◽

R. D. Reichert ◽

W. E. Roe

Keyword(s):

Amino Acid ◽

Dry Matter ◽

Soybean Hulls ◽

Matter Energy

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Effect of dietary protein level and salinity on growth, survival, enzymatic activities and amino-acid composition of the white shrimp Litopenaeus vannamei (Boone, 1931) juveniles

Crustaceana ◽

10.1163/15685403-00003390 ◽

2015 ◽

Vol 88 (1) ◽

pp. 82-95 ◽

Cited By ~ 8

Author(s):

Liying Sui ◽

Guannan Ma ◽

Yuangao Deng

Keyword(s):

Amino Acid ◽

Protein Content ◽

Muscle Tissue ◽

Litopenaeus Vannamei ◽

Dietary Protein ◽

Protein Level ◽

White Shrimp ◽

Lower Growth ◽

Protein Levels ◽

Dietary Protein Level

Litopenaeus vannamei (Boone, 1931) postlarvae with average initial body weight of 0.089 g were reared in 75-litre PVC tanks for 40 days at salinities of 30 and 60 g l−1. The shrimps were fed compound feed containing protein levels of 35, 40, 45 and 50%, respectively. Salinity had a remarkable effect on growth and survival of L. vannamei juveniles. Higher survival rate and lower growth were observed at 60 g l−1 salinity. Dietary protein level affected the survival and growth of juveniles at both salinities, increased with dietary protein levels in the range of 35 to 45%, but decreased slightly with 50% dietary protein. Broken line analysis showed that the estimated optimal dietary protein levels at salinities of 30 and 60 g l−1 were 45.93 and 46.74%, respectively. Higher salinity resulted in an increased moisture content, ash and crude protein content in the shrimp muscle tissue. The amino acid contents in the shrimp muscle tissue were generally higher at 60 g l−1 salinity and increased dietary protein level led to higher protein content, except with 50% dietary protein. At salinity 60 g l−1, the soluble protein content and activities of glutamic oxalacetic transferase (GOT) and glutamic pyruvic transaminase (GPT) in shrimp muscle tissue were higher, while catalase (CAT) activities were lower. Farming of L. vannamei at a marginal culture salinity (60 g l−1) is feasible though the shrimps were likely exposed to stressful conditions. Reduced growth rate at higher salinity may be attributed to the higher total ammonium (TAN) concentration in the culture medium and extra energy consumption for osmoregulation at hypersaline conditions.

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Molecular cloning of a β-glucan pattern-recognition lipoprotein from the white shrimp Penaeus (Litopenaeus) vannamei: correlations between the deduced amino acid sequence and the native protein structure

Developmental & Comparative Immunology ◽

10.1016/j.dci.2003.11.008 ◽

2004 ◽

Vol 28 (7-8) ◽

pp. 713-726 ◽

Cited By ~ 48

Author(s):

M Romo-Figueroa

Keyword(s):

Pattern Recognition ◽

Protein Structure ◽

Amino Acid ◽

Amino Acid Sequence ◽

Molecular Cloning ◽

Litopenaeus Vannamei ◽

White Shrimp ◽

Native Protein

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The Efficacy of Mineral-Amino Acid Complex (Zn, Mn, Cu, Fe and Se) on White Shrimp, Litopenaeus vannamei, Diets

Aquacultura Indonesiana ◽

10.21534/ai.v16i1.6 ◽

2015 ◽

Vol 16 (1) ◽

Author(s):

Orapint Jintasataporn ◽

Terry Ward ◽

Srinoy Chumkam ◽

Oratai Jintasataporn

Keyword(s):

Amino Acid ◽

Litopenaeus Vannamei ◽

White Shrimp ◽

Acid Complex ◽

Amino Acid Complex

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Molecular Cloning and Sequence Analysis of Peroxiredoxin cDNA from American White Shrimp (Litopenaeus vannamei)

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.343-344.1255 ◽

2011 ◽

Vol 343-344 ◽

pp. 1255-1264 ◽

Cited By ~ 1

Author(s):

Xiao Long Liu ◽

Qian Yun Xi ◽

Lin Yang ◽

Hong Yi Li ◽

Qing Yan Jiang ◽

...

Keyword(s):

Amino Acid ◽

Litopenaeus Vannamei ◽

Untranslated Region ◽

Fenneropenaeus Chinensis ◽

Physiological Role ◽

Peptide Sequence ◽

White Shrimp ◽

Scylla Serrata ◽

Rt Pcr ◽

Signal Peptide Sequence

Peroxiredoxin (Prx) is an antioxidant protein, which protects organisms against various oxidative stresses. In this study, we isolated Peroxiredoxin cDNA from the muscle tissues of American white shrimp, Litopenaeus vannamei, using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The full-length cDNA consists of 962-bp, which includes a 49-bp 5′-untranslated region (UTR), a 316-bp 3′-untranslated region, and a 597-bp open reading frame (ORF) encoding 198 amino acids. The signal peptide sequence was not found in this cDNA. We aligned the deduced amino acid sequence with the known amino acid sequences of Fenneropenaeus indicus, Fenneropenaeus chinensis, Marsupenaeus japonicus, Scylla serrata, Drosophila melanogaster, Bos taurus, Branchiostoma belcheri, Anoplopoma fimbria, and Rattus norvegicus, and the sequence similarities scores were found to be 97%, 96%, 95%, 83%, 72%, 70%, 80%, 81%, and 75%, respectively. We also found 2-cysteine (Cys) residues in this peroxiredoxin sequence. The RT-PCR analysis revealed that the peroxiredoxin mRNA was expressed in the gills, hepatopancreas, muscles, intestine, and hemocytes. Studies using this newly cloned peroxiredoxin gene from Litopenaeus vannamei will add to the existing knowledge base on the physiological role of peroxiredoxin in shrimp species.

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