Water quality and microbial community structure in juvenile Atlantic cod (Gadus morhua L.) cultures

Currently, black-odor river has received great attention in China. In this study, the micro-nano bubble technology (MBT) was used to mitigate the water pollution rapidly and continuously by increasing the concentration of dissolved oxygen (DO) in water. During treatment, the concentration of DO increased from 0.60 mg/L to over 5.00 mg/L, and the oxidation reduction potential (ORP) also changed from a negative value to over 100.00 mV after only five days aeration. High throughput pyrosequencing technology was employed to identify the microbial community structure. At genus level, the dominant bacteria were anaerobic and nutrient-loving microbes (e.g., Arcobacter sp., Azonexus sp., and Citrobacter sp.) before, and the relative abundances of aerobic and functional microbes (e.g., Perlucidibaca sp., Pseudarcicella sp., Rhodoluna sp., and Sediminibacterium sp.) were increased after treatment. Meanwhile, the water quality was significantly improved with about 50% removal ratios of chemical oxygen demand (CODCr) and ammonia nitrogen (NH4+-N). Canonical correspondence analysis (CCA) results showed that microbial community structure shaped by COD, DO, NH4+-N, and TP, CCA1 and CCA2 explained 41.94% and 24.56% of total variances, respectively. Overall, the MBT could improve the water quality of urban black-odor river by raising the DO and activate the aerobic microbes.

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Aquaculture Water Quality Improvement by Mixed Bacillus and Its Effects on Microbial Community Structure

10.21203/rs.3.rs-1057159/v1 ◽

2021 ◽

Author(s):

Xue Li ◽

Tianie Wang ◽

Baorong Fu ◽

Xiyan Mu

Keyword(s):

Water Quality ◽

Community Structure ◽

Microbial Community ◽

Relative Abundance ◽

Bacillus Megaterium ◽

Microbial Community Structure ◽

Pathogenic Bacteria ◽

Phosphorus Compounds ◽

Nitrogen And Phosphorus ◽

Aquaculture Water

Abstract Nutrients nitrogen and phosphorus pollution in aquaculture is one of the greatest challenges threatening the survival of aquatic organisms, which requires efficient and sustainable remediation approach. Microbial remediation, especially the application of probiotics, has recently gained popularity in improving the water quality and maintaining the health condition of aquatic animals. In the present study, two groups of mixed Bacillus (Bacillus megaterium and Bacillus subtilis (A0+BS) and Bacillus megaterium and Bacillus coagulans (A0+BC)) were applied to aquaculture system of Crucian carp to improve the treatment of nitrogenous and phosphorus compounds. The effects of mixed Bacillus on water quality, and the structure and function of microbial communities in aquaculture water were investigated. Our results showed that the improvement effect of mixed Bacillus A0+BS on water quality was better than that of A0+BC, and the NH4+-N, NO2--N, NO3--N and total phosphorus (TP) concentrations were reduced by 46.3%, 76.3%, 35.6%, and 80.3%, respectively. In addition, both groups of mixed Bacillus increased the diversity of bacterial community and decreased the diversity of fungal community. Microbial community analysis showed that mixed Bacillus A0+BS increased the relative abundance of bacteria related with nitrogen and phosphorus removal, e.g., Proteobacteria, Actinobacteria, Comamonas, and Stenotrophomonas, but decreased the relative abundance of pathogenic bacteria (Acinetobacter and Pseudomonas) and fungi (Epicoccum and Fusarium). The redundancy analysis showed that NH4+-N, NO2--N, and TP were primary environmental factors affecting the microbial community in aquaculture water. PICRUST analysis indicated that all functional pathways in the treatment groups were up-regulated, and all pathways in A0+BS group were richer than those in other groups. These results indicated that mixed Bacillus A0+BS addition produced good results in reducing nitrogenous and phosphorus compounds and shaped a favorable microbial community structure to further improve water quality.

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Effects of stocking density of gray mullet Mugil cephalus on water quality, growth performance, nutrient conversion rate, and microbial community structure in the white shrimp Litopenaeus vannamei integrated system

Aquaculture ◽

10.1016/j.aquaculture.2018.07.018 ◽

2018 ◽

Vol 496 ◽

pp. 123-133 ◽

Cited By ~ 10

Author(s):

Manh N. Hoang ◽

Phuoc N. Nguyen ◽

Duy V.B. Le ◽

Dung V. Nguyen ◽

Peter Bossier

Keyword(s):

Water Quality ◽

Community Structure ◽

Microbial Community ◽

Growth Performance ◽

Microbial Community Structure ◽

Litopenaeus Vannamei ◽

Conversion Rate ◽

Stocking Density ◽

Integrated System ◽

White Shrimp

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Photosynthetic purple sulfur bacterium Marichromatium purpuratum RuA2 induces changes in water quality parameters, the occurrence of sulfonamide resistance gene and microbial community structure of marine aquaculture

Aquaculture ◽

10.1016/j.aquaculture.2018.04.055 ◽

2018 ◽

Vol 493 ◽

pp. 68-78 ◽

Cited By ~ 6

Author(s):

Iau-Iun Shiung ◽

Man-Jung Chang ◽

Yi-Tang Chang ◽

Shinn-Lih Yeh ◽

Su-Jung Chang ◽

...

Keyword(s):

Water Quality ◽

Community Structure ◽

Microbial Community ◽

Resistance Gene ◽

Microbial Community Structure ◽

Quality Parameters ◽

Purple Sulfur Bacterium ◽

Marine Aquaculture ◽

Sulfonamide Resistance ◽

Sulfonamide Resistance Gene

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Microbial Community Structure and Diversity in Drinking Water Supply, Distribution Systems as Well as Household Point of Use Site in Addis Ababa City, Ethiopia

10.21203/rs.3.rs-153906/v1 ◽

2021 ◽

Author(s):

Bayable Atanfu ◽

Adey Desta ◽

Fassil Assefa

Keyword(s):

Water Quality ◽

Drinking Water ◽

Community Structure ◽

Microbial Community ◽

Water Supply ◽

Microbial Community Structure ◽

Distribution Systems ◽

Drinking Water Quality ◽

Source Water ◽

Intermittent Water Supply

Abstract BackgroundUnderstanding ecology of microbiomes in drinking water distribution systems is the most important notion in delivering safe drinking water. Despite cultivation-based methods routinelyemployed in monitoring drinking water quality, cultivation of specific indicator organisms alone is not always guarantee for assuring safe drinking water delivery. The presence of complex microbiomes in drinking water distribution systems affects treatment effectiveness leading to poor quality water which as a result affects health of human and animals. Drinking water treatment and distribution systems harbor various microbiota despite efforts made in improving water infrastructures and several waterborne diseases become serious problems in the water industry, specially, in developing Countries. Intermittent water supply, long-time of water storage, low water pressure in distribution systems, storage tankers and pipes as well as contaminated source water are among many of the factors responsible for low drinking water quality which in turn affecting health of people. The aim of this study was to explore microbial diversity and structure in water samples collected from source water, treated water, reservoirs, and several household points of use locations (taps). High throughput Illumina sequencing technology was employed by targeting V4 region of 16S rRNA following Illumina protocol to analyze the community structure of bacteria. ResultsThe core dominating taxa were Proteobacteria followed by Firmicutes, Bacteroidetes and Actinobacteria. Gamma proteobacteria were dominant among other Proteobacterial classes across all sampling points. Opportunistic bacterial genera such as Pseudomonas, Legionella, Klebsiella, Escherichia, Actinobacteria, as well as eukaryotic microbes like Cryptosporidium, Hartmanella, Acanthamoeba, Aspergillus, and Candida were also the abundant taxa found alongthe distribution systems. The shift in microbial community structure from source to point of uselocations were influenced by factors such as residual free chlorine, intermittent water supply andlong-time storage at the household. The shift in microbial community structure from source to point of use locations were influenced by factors such as residual free chlorine, intermittent water supply and long-time storage at the household.ConclusionsThe complex microbiota which was present in different sample sites receiving treated water from the two treatment plants (Legedadi and Gefersa) starting from source water to household point of consumption across the distribution systems in Addis Ababa brings drinking water quality problem which further causes significant health problems to both human and animal health. Treatment ineffectiveness, disinfection inefficiency, poor maintenance actions, leakage of sewage and other domestic wastes are few among many other factors responsible for degraded drinking water quality in this study putting health at high risk which, this, leads to morbidity and mortality. Findings of this research provide important and bassline information to understand the microbial profiles of drinking water along source water and distribution systems.

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