Effects of dietary carbohydrate sources on growth performance, glycogen accumulation, insulin signaling pathway and hepatic glucose metabolism in largemouth bass, Micropterus salmoides

Aquaculture ◽  
2019 ◽  
Vol 513 ◽  
pp. 734391 ◽  
Author(s):  
Songlin Li ◽  
Chunyan Sang ◽  
An Wang ◽  
Jiacan Zhang ◽  
Naisong Chen
Keyword(s):  
Glucose Metabolism ◽  
Growth Performance ◽  
Signaling Pathway ◽  
Insulin Signaling ◽  
Largemouth Bass ◽  
Micropterus Salmoides ◽  
Dietary Carbohydrate ◽  
Glycogen Accumulation ◽  
Hepatic Glucose Metabolism ◽  
Hepatic Glucose

Starch in aquafeeds: the benefits of a high amylose to amylopectin ratio and resistant starch content in diets for the carnivorous fish, largemouth bass (Micropterus salmoides)

2020 ◽  
Vol 124 (11) ◽  
pp. 1145-1155
Author(s):  
Songlin Li ◽  
Chunyan Sang ◽  
Giovanni M. Turchini ◽  
An Wang ◽  
Jiacan Zhang ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Largemouth Bass ◽  
Resistant Starch ◽  
Starch Content ◽  
Micropterus Salmoides ◽  
Hepatic Glycogen ◽  
Insulin Pathway ◽  
Glycogen Accumulation ◽  
Dietary Starch ◽  
High Amylose

AbstractAquafeeds for carnivorous species face a nutritional–technological conundrum: containing sufficient starch to meet specific manufacturing requirements for binding, extrusion and expansion, but ideally containing as little starch as possible owing to their limited ability to utilise carbohydrates. The present study evaluated the effects of dietary starch with different amylose to amylopectin ratios and resistant starch contents on growth performance, hepatic glycogen accumulation and glucose metabolism of an important cultured carnivorous finfish, largemouth bass (Micropterus salmoides). A common starch source (α-cassava starch (CS)) was tested as is or after being enzymatically de-branched at three different inclusion levels in diets for largemouth bass. Results showed that the increased dietary starch levels compromised performance and high dietary α-CS content led to obvious liver damage. However, the growth performances of fish fed the diets with de-branched starch (DS) were improved, and no manifest liver damages were observed even at the higher inclusion level. The increasing dietary starch contents significantly increased hepatic glycogen accumulation, but not when DS was used. High dietary starch content, without regard to starch sources, had no effect on the expression of glucose metabolism-related genes, except for down-regulation of insulin receptor expression. However, the use of dietary DS promoted the expression of genes involved in the insulin pathway and glycolysis. In conclusion, this study showed that the use of starch sources with a high amylose to amylopectin ratio and resistant starch in the feed for cultured carnivorous finfish could alleviate the hepatic glycogen deposition through regulating the insulin pathway and glycolysis.

Resveratrol inclusion alleviated high dietary carbohydrate induced glycogen deposition and immune response of largemouth bass, Micropterus salmoides

2021 ◽  
pp. 1-33 ◽  
Author(s):  
Yijun Liu ◽  
Ning Liu ◽  
An Wang ◽  
Naisong Chen ◽  
Songlin Li
Keyword(s):  
Immune Response ◽  
Inflammatory Cytokines ◽  
Largemouth Bass ◽  
Micropterus Salmoides ◽  
Sirtuin 1 ◽  
Hepatic Glycogen ◽  
Dietary Carbohydrate ◽  
Glycogen Accumulation ◽  
High Carbohydrate ◽  
Glycogen Deposition

Abstract Excessive hepatic glycogen accumulation commonly impairs hepatocytes function, and further produces negative effects on growth and health status of carnivorous fish. A 9-week feeding trial was conducted to explore the potential regulation of resveratrol on high carbohydrate induced glycogen deposition and immune response of largemouth bass. Results showed that high dietary carbohydrate (10% inclusion of starch) led to hepatic glycogen accumulation and postprandial hyperglycemia compared to the diet with 5% starch, which was both alleviated with the inclusion of resveratrol. The use of resveratrol promoted the expression of sirtuin 1, which was down-regulated by high dietary carbohydrate. Meanwhile, resveratrol inclusion promoted the expression of genes involved in insulin pathway and glycolysis, and inhibited the expression of gluconeogenesis related genes. Additionally, high dietary carbohydrate significantly reduced lysozyme content but increased complement C4 content, which were both reversed with resveratrol supplementation. Meanwhile, resveratrol inclusion inhibited the expression of pro-inflammatory cytokines, but promoted anti-inflammatory cytokines expression, compare to the high carbohydrate treatment. In conclusion, resveratrol inclusion was beneficial in alleviating high dietary carbohydrate induced glycogen accumulation and immune response in largemouth bass.

scholarly journals Dietary Lysine Regulates Body Growth Performance via the Nutrient-Sensing Signaling Pathways in Largemouth Bass (Micropterus salmoides)

2020 ◽  
Vol 7 ◽  
Author(s):  
Wenqiang Wang ◽  
Yujue Xu ◽  
Shuyan Chi ◽  
Peng Yang ◽  
Kangsen Mai ◽  
...  
Keyword(s):  
Growth Performance ◽  
Signaling Pathway ◽  
Largemouth Bass ◽  
Micropterus Salmoides ◽  
Nutrient Utilization ◽  
Diet Group ◽  
Nutrient Sensing ◽  
Body Growth ◽  
Fish Growth ◽  
Human Beings

Lysine is known to be a functional nutrient from yeast to human beings. However, the mechanisms for lysine to regulate body growth and metabolism in fish species are far from fully illuminated. In order to elucidate the molecular effects of lysine on growth performance and metabolism, largemouth bass (Micropterus salmoides), which is extremely sensitive to protein composition, was chosen as the experimental model. Juvenile largemouth bass (4.16 ± 0.02 g) were fed with the lysine control diet (LC diet), the lysine supplementation diet (LS diet), and the low lysine diet (LL diet) for 8 weeks. The results showed that the LS diet group significantly increased fish growth, feed efficiency, and nutrient retention compared with those of the LL diet group in largemouth bass. Moreover, compared to the LL diet, lysine supplementation also elevated the plasma total protein, total EAA, total AA, and some individual AA concentrations. Mechanistically, lysine supplementation had a significant effect to decrease the mRNA expression levels of peptide and AA transporters induced by lysine restriction. Lysine supplementation also had an important impact on regulating the target of rapamycin (TOR) signaling pathway. More importantly, the key regulators in the amino acid response (AAR) signaling pathway were also down-regulated by lysine supplementation. Our results provide a clear elucidation of how dietary lysine affected growth performance, physiological and biochemical responses, and signaling responses and represent a sound foundation for using lysine to improve the nutrient utilization of poultry by-product meal in largemouth bass.

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